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  • Triticum aestivum  (21)
  • Pisum
  • Springer  (34)
  • Springer Science + Business Media
  • 1980-1984  (34)
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  • Springer  (34)
  • Springer Science + Business Media
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Year
  • 1
    Electronic Resource
    Electronic Resource
    The effects of managanese and nitrate supply on the levels of phenolics and lignin in young wheat plants (1984)
    Springer
    Plant and soil 81 (1984), S. 437-440 
    Details
    ISSN: 1573-5036
    Keywords: Lignin ; Manganese ; NO 3 − Phenols ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Managanese deficiency (〈 18 μg g−1 Mn) resulted in decreased levels of phenols in wheat shoots and decreased levels of lignins in both roots and shoots. These observed reductions in phenol contents was due largely to a decrease in the alkaline labile phenol component. Levels of nitrate supplied in solution influenced both phenol and lignin production; high nitrate levels (15 mM) resulted in a reduction in phenol and lignin in the shoot but stimulated lignin production in root tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02323058
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  • 2
    Electronic Resource
    Electronic Resource
    Role of ATP in nitrite reduction in roots of wheat and pea (1981)
    Springer
    Planta 152 (1981), S. 234-238 
    Details
    ISSN: 1432-2048
    Keywords: ATP and nitrite reduction ; Glucose-6-phosphate ; Nitrite reduction ; Pisum ; Plastids, nitrite reduction ; Root, plastids ; Triticum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Excised wheat (Triticum aestivum L.) and field pea (Pisum arvense L.) roots, incubated under anaerobic conditions or in the presence of uncouplers of oxidative phosphorylation [2,4-dinitrophenol (DNP), carbonylcyanide-m-chlorophenylhydrazone, pentachlorophenol] accumulated nitrite as a result of an inhibition of nitrite reduction. In isolated root plastids, nitrite reduction was dependent on a supply of glucose-6-phosphate (G6P) and did not require ATP. The estimated Km value for glucose 6-phosphate was 1.25 mM. Glucose and fructose-1,6-diphosphate were ineffective substrates for nitrate reduction. Anaerobic conditions and treatment with DNP, which would result in a cessation of ATP production by the mitochondria and a stimulation of glycolysis via the “Pasteur effect”, were shown to decrease the G6P content of excised roots of wheat and pea. A negative correlation was observed between the level of G6P and nitrite accumulation on root tissues. It is proposed that an interruption in the supply of G6P to the root plastid under these conditions would result in an inhibition of nitrite reduction leading to nitrite accumulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385149
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  • 3
    Electronic Resource
    Electronic Resource
    Characterization of the multigene family coding for HMW glutenin subunits in wheat using cDNA clones (1983)
    Springer
    Theoretical and applied genetics 67 (1983), S. 87-96 
    Details
    ISSN: 1432-2242
    Keywords: HMW glutenin subunit genes ; cDNA clones ; Tandem DNA repeats ; Chromosomal location ; Gene copy number ; Wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary cDNA clones encoding wheat HMW glutenin subunits have been isolated from a cDNA bank made to poly A+ RNA from developing wheat endosperm var. Chinese Spring. One such clone, pTag 1290, has enabled us to identify the HMW glutenin mRNA species. The DNA sequence of this clone has been partially determined and it contains several tandem DNA repeats. The sequence is discussed in relation to the generation of the HMW glutenin subunit gene family. Analysis of the organization of the HMW glutenin sequences in the wheat genome revealed that the genes encoding HMW glutenin subunits exist in low copy number and are located on the long arm of each of the homoeologous group 1 chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303930
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  • 4
    Electronic Resource
    Electronic Resource
    A role for managanese in the resistance of wheat plants to take-all (1984)
    Springer
    Plant and soil 78 (1984), S. 441-444 
    Details
    ISSN: 1573-5036
    Keywords: Gaeumannomyces graminis vartritici ; Hay-die ; Manganese deficiency ; Take-all ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The hypothesis that wheat plants deficient in managenese are predisposed to infection byGaeumannomyces graminis is outlined, and a test of the hypothesis in a soil system is reported. The results supported the hypothesis: wheat plants growing in managanese-deficient soil, although not showing foliar symptoms, were markedly more susceptible to infection; plant analysis confirmed the nutrient status of the plants. A review of the literature on take-all in wheat coupled with the results of our experiments suggests a reinterpretation of the etiology of this disease, since those edaphic factors which promote infection by this organism are those which also render managese unavailable to the host. Managenese nutrition is proposed as a common factor in many of the environmental conditions which influence the host-pathogen balance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02450379
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  • 5
    Electronic Resource
    Electronic Resource
    Cell length, light and14C-labelled indol-3yl-acetic acid transport inPisum satisum L. andPhaseolus vulgaris L (1980)
    Springer
    Planta 149 (1980), S. 327-331 
    Details
    ISSN: 1432-2048
    Keywords: Auxin transport ; Cell length ; Light and auxin transport ; Phaseolus ; Pisum ; Transport (auxin)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The putative auxin-transporting cells of the intact herbaceous dicotyledon are the young, differentiating vascular elements. The length of these cells was found to be considerably greater in dwarf (Meteor) than in tall (Alderman) varieties ofPisum sativum L., and to be greater in etiolated than in light-grown plants ofP. sativum cv Meteor andPhaseolus vulgaris L. cv Mexican Black. Under given light conditions during transport these large differences in cell length did not influence the shapes of the transport profiles or the velocity of transport of14C-labelled indol-3yl-acetic acid (IAA) applied to the apical bud. However, in both etiolated and light-grown bean and dwarf pea plants the velocity of transport in darkness was ca. 25% lower than that in light. Under the same conditions of transport velocities in bean were about twice those observed in the dwarf pea. Exposure to light during transport increased the rate of export of14C from the labelled shoot apex in green dwarf pea plants but not in etiolated plants. The light conditions to which the plants were exposed during growth and transport had little effect on the rates of uptake of IAA from the applied solutions. The results indicate that the velocity of auxin transport is independent of the frequency of cell-to-cell interfaces along the transport pathway and it is suggested that in intact plants auxin transport is entirely symplastic.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00571165
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  • 6
    Electronic Resource
    Electronic Resource
    Influence of winter and summer growth conditions on leaf membrane lipids of Pisum sativum L. (1983)
    Springer
    Planta 157 (1983), S. 218-223 
    Details
    ISSN: 1432-2048
    Keywords: Chloroplast membranes ; Fatty-acid composition (leaf membranes) ; Pisum ; Seasonal variation (fatty acids) ; Thylakoid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The lipid composition and degree of unsaturation of fatty acids has been measured for membranes isolated from leaves of Pisum sativum grown under either summer or winter conditions. Although the lipid-class composition was not significantly changed for the two growth conditions the degree of unsaturation of the fatty acids was greater for winter than summer plants. The difference in unsaturation was evident with all lipid classes of the non-thylakoid membranes including the galactolipids of the chloroplast envelope. In contrast, both the relative amounts of lipid classes and degree of saturation were not greatly changed for summer and winter thylakoids with the exception that phosphatidylglycerol had a greater linolenic acid (18:3) content for the thylakoids of winter grown leaves. However, a striking difference was found for the total acyl lipid to chlorophyll ratio for thylakoids isolated from summer or winter plants, with the former producing a lower ratio than the latter growth conditions. The above changes in lipid composition of chloroplast membranes are discussed in terms of optimizing their functional activities under the different growth conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00405185
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  • 7
    Electronic Resource
    Electronic Resource
    Stomatal responses of Argenteum — a mutant of Pisum sativum L. with readily detachable leaf epidermis (1982)
    Springer
    Planta 155 (1982), S. 146-153 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Cytokinin ; Mutant (barley) ; Pisum ; Potassium and stomata protoplast ; Stomata (epidermis)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Epidermis is easily detached from both adaxial and abaxial surfaces of leaf four of the Argenteum mutant of Pisum sativum L. The isolated epidermis has stomata with large, easily-measured pores. Hairs and glands are absent. The density of stomata is high and contamination by mesophyll cells is low. In the light and in CO2-free air, stomata in isolated adaxial epidermis of Argenteum mutant opened maximally after 4 h incubation at 25°C. The response of stomata to light was dependent on the concentration of KCl in the incubation medium and was maximal at 50 mol m-3 KCl. Stomata did not respond to exogenous kinetin, but apertures were reduced by incubation of epidermis on solutions containing between 10-5 and 10-1 mol m-3 abscisic acid (ABA). The responses of stomata of Argenteum mutant to light, exogenous KCl, ABA and kinetin were comparable with those described previously for stomata in isolated epidermis of Commelina communis. A method for preparing viable protoplasts of guard cells from isolated epidermis of Argenteum mutant is described. The response of guard cell protoplasts to light, exogenous KCl, ABA and kinetin were similar to those of stomata in isolated epidermis except that the increase in volume of the protoplasts in response to light was maximal at a lower concentration of KCl (10 mol m-3) and that protoplasts responded more rapidly to light than stomata in isolated epidermis. The protoplasts did not respond to exogenous kinetin, but when incubated for 1 h in the light and in CO2-free air on a solution containing 10-3 mol m-3 ABA, they decreased in volume by 30%. The advantages of using epidermis from Argenteum mutant for experiments on stomatal movements are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392545
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  • 8
    Electronic Resource
    Electronic Resource
    The initiation of legumin synthesis in immature embryos of Pisum sativum L. grown in vivo and in vitro (1980)
    Springer
    Planta 149 (1980), S. 454-460 
    Details
    ISSN: 1432-2048
    Keywords: Embryo culture ; Enzyme-linked immunosorbent assay (ELISA) ; Legumin ; Pisum ; Protein (storage) ; Storage protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A highly sensitive immunoassay has been used for the detection of a major storage protein, legumin, in embryos of Pisum sativum L.; with this technique nanogram quantities could be measured. In the two varieties tested, legumin could be detected in embryos in vivo, when they had attained a fresh weight of 2·10-3 g and 3·10-3 g, respectively. Contrary to earlier claims, embryos cultured in vitro were shown to be capable of initiating legumin synthesis. This capacity to initiate legumin synthesis was confirmed by two-dimensional isoelectric focusing-electrophoresis and fluorography; embryos harvested before initiation of legumin synthesis and cultured in radioactive medium were shown to have synthesized legumin subunits. The amounts of legumin and total protein synthesized per unit fresh weight were consistently greater in vitro than in equivalent embryos grown in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385747
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  • 9
    Electronic Resource
    Electronic Resource
    The influence of small direct electric currents on the transport of auxin in intact plants (1980)
    Springer
    Planta 150 (1980), S. 431-434 
    Details
    ISSN: 1432-2048
    Keywords: Auxin transport ; Electric current ; Pisum ; Potential gradients
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract When a d.c. potential of 9.0 V was applied to the stem of intact pea seedlings (Pisum sativum L. cv. Meteor and cv. Alderman) via 10 mM KCl-soaked filter paper electrodes placed ca. 50 mm apart the stem passed a steady current of 15–20 μA (resistance ca. 100 kΩ cm-1). The basipetal transport of [1-14C]IAA applied to the apical bud was completely inhibited over the portion of the stem through which current flowed and 14C-labelled compounds accumulated in the vicinity of the upper electrode. The inhibition of transport was independent of the polarity of the applied potential. The basipetal transport of IAA in the stem above the electrode was not affected. Labelled auxin accumulated at the upper electrode both as unchanged IAA and as a compound tentatively identified as indol-3yl-acetyl aspartic acid (IAAsp). These compounds were only slowly remobilised when the current was interrupted. However, the ability of the transport system to move freshly-applied IAA was rapidly and fully restored when the potential was removed. No injury to the plant was detected after maintaining a current flow for up to 72 h. No leakage of 14C-labelled compounds into the KCl solution bathing the electrodes was detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390181
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  • 10
    Electronic Resource
    Electronic Resource
    Characterisation of the storage protein subunits synthesised in vitro by polyribosomes and RNA from developing pea (Pisum sativum L.) (1980)
    Springer
    Planta 148 (1980), S. 49-56 
    Details
    ISSN: 1432-2048
    Keywords: Legumin ; Pisum ; Protien synthesis ; RNA ; Storage protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Evidence is presented to show that legumin, the major storage protein in Pisum, is synthesised in vitro by the wheat germ and reticulocyte lysate systems, from polyribosomes and mRNA isolated from developing pea seeds. While legumin isolated from mature pea seeds consists of 40,000 and 20,000 MW subunits, the in vitro legumin is synthesised as a 60,000 MW precursor consisting of covalently linked 40,000 and 20,000 MW subunits. The implications of these findings are discussed in relationship to studies with other systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385441
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