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1

Digitale Medien

Gas chromatography mass spectrometry computer analysis of volatile halogenated hydrocarbons in man and his environment - a multimedia environmental study (1980)

Barkley, Jane ; Bunch, John ; Bursey, Joan T. ; [weitere]

Chichester : Wiley-Blackwell

Biological Mass Spectrometry 7 (1980), S. 139-147

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ISSN: 1052-9306

Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Notizen: As part of a study to make a comparative analysis of selected halogenated compounds in man and the environmental media, a quantitative gas chromatographic mass spectrometric analysis of the levels of the halogenated compounds found in the breath, blood and urine of an exposed population (Old Love Canal area, Niagara, New York) and their immediate environment (air and water) was undertaken. In addition, levels of halogenated hydrocarbons in air samples taken in the general Buffalo, Niagara Falls area were determined.

Zusätzliches Material: 8 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bms.1200070402

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2

Digitale Medien

Perturbation of the switch-on of transcriptase activity in intermediate subviral particles from reovirus (1982)

Borsa, J. ; Sargent, M. D. ; Ewing, D. D. ; [weitere]

New York, NY [u.a.] : Wiley-Blackwell

Journal of Cellular Physiology 112 (1982), S. 10-18

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ISSN: 0021-9541

Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Medizin

Notizen: Intermediate subviral particles (ISVP) derived from reovirus represent a simple model system for the switch-on of transcriptase function. In such particles the endogenous transcriptase is present in a switched-off form, one step removed from the switched-on state. Switch-on of transcriptase function is an active process in this system and can be triggered by K+ ions. A variety of agents which affect gene expression in cells were tested for an effect on switch-on in ISVP. Marked effects on switch-on in ISVP were observed with a diverse group of test agents, including DMSO and other solvents, BUdR, TdR, caffeine, theophylline, and temperature. The correlation in response between ISVP and cells suggests that the ISVP system may be useful as a model for studying the biochemical mechanisms underlying the perturbative effects of such agents on gene expression in cells.

Zusätzliches Material: 14 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jcp.1041120104

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3

Digitale Medien

Inhibition of the metabolism of ram spermatozoa by derivatives of α-chlorohydrin (1981)

Brown-Woodman, P. D. C. ; White, I. G. ; Ridley, D. D.

New York, NY : Wiley-Blackwell

Gamete Research 4 (1981), S. 203-217

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ISSN: 0148-7280

Schlagwort(e): α-chlorohydrin ; antifertility agent ; ram ; sperm metabolism ; Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie

Notizen: The effects of the male antifertility agent, α-chlorohydrin, six of its derivatives, and glycidol were studied on the metabolism of washed ram spermatozoa in vitro with fructose as substrate. The α-chlorohydrin derivatives were the amino, the phosphorylated, and four glycol-bridge (ketal) compounds. All compounds except glycidol, in a concentration between 0.1 and 100 mM, reduced the aerobic glycolsis and/or oxidation of fructose. However, there was not a high correlation between the ability of these compounds to inhibit the metabolism of ram spermatozoa in vitro and their antifertility activity when administered to male rats. Other factors are clearly involved in their antifertility activity, eg, the concentration of the compounds in the epididymis and their conversion of either more or less spermicidal compounds in the body.

Zusätzliches Material: 1 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/mrd.1120040305

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4

Digitale Medien

Morphological and ultrastructural characterization of mammalian spermatozoa processed for flow cytometric DNA analyses (1984)

Garner, D. L. ; Johnson, L. A. ; Lake, S. ; [weitere]

New York, NY : Wiley-Blackwell

Gamete Research 10 (1984), S. 339-351

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ISSN: 0148-7280

Schlagwort(e): spermatozoa ; flow cytometry ; DNA staining ; nuclear morphology ; ultrastructure ; mammals ; Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie

Notizen: The morphological and ultrastructural changes that occur during preparation of porcine, bovine, and murine spermatozoa for flow cytometric quantification of the relative DNA content of the X- and Y-chromosome-bearing sperm populations were examined. Ejaculated spermatozoa from the boar and bull were washed using a series of dimethyl sulfoxide (DMSO) solutions prior to fixation, whereas the epididymal mouse spermatozoa were washed only in phosphate-buffered saline (PBS). Spermatozoa from all three species were then fixed in ethanol and processed for fluorochrome staining by a treatment regimen consisting of sulfhydryl reduction and proteolysis. The processed sperm nuclei were stained for DNA with the fluorochrome, 4′-6-diamidino-2-phenylindole (DAPI) before quantification by flow cytometry. Scanning and transmission electron micrographs of sperm heads taken at various steps of the preparation and staining procedures show 1) that the rigorous washing procedure disrupted the plasma and outer acrosomal membranes, 2) that ethanol fixation resulted in removal of the outer membranes and disintegration of the nuclear envelope, and 3) that thiol and proteolysis treatment removed the remaining cellular organelles including the tail and rapidly induced partial decondensation of the tightly packed chromatin. Sequential micrographs showed that the nuclear matrix of all three species increased in thickness about twofold during the preparation and staining. Consequently, the harsh procedures currently used for quantitative staining of DNA for high-resolution flow cytometric analyses destroy most cellular organelles and thereby prevent simultaneous characterization of DNA content and other sperm cell constituents.

Zusätzliches Material: 6 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/mrd.1120100402

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5

Digitale Medien

Bubble formation in animals. I. Physical factorsThis work has been aided since July 1, 1942, by grants from the Committee on Aviation Medicine of the National Research Council carried out under contract recommended by the Committee on Medical Reseach between the Office of Scientific Research and Development and Princeton University. (1944)

Harvey, E. Newton ; Barnes, D. K. ; McElroy, W. D. ; [weitere]

Philadelphia : Wiley-Blackwell

Journal of Cellular and Comparative Physiology 24 (1944), S. 1-22

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ISSN: 0095-9898

Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Medizin

Zusätzliches Material: 4 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jcp.1030240102

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6

Digitale Medien

Bubble formation in animals. II. Gas nuclei and their distribution in blood and tissuesThis work has been aided since July 1, 1942, by grants from the Committee on Aviation Medicine of the National Research Council carried out under contract recommended by the Commitee on Medical Reseach between the Office of Scientific Research and Development and Princeton University. Reference will not be made to progress reports or other literature of the Committee on Aviation Medicine. (1944)

Harvey, E. Newton ; Whiteley, A. H. ; McElroy, W. D. ; [weitere]

Philadelphia : Wiley-Blackwell

Journal of Cellular and Comparative Physiology 24 (1944), S. 23-34

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ISSN: 0095-9898

Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Medizin

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jcp.1030240103

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7

Digitale Medien

15N and 13C NMR study of the effects of hydrogen bonding and protonation in linear schiff bases: Models for structural studies of rhodopsin and bacteriorhodopsinPart 4 of the series ‘Spectral Studies of Visual Pigments and Related Systems.’ For part 5, see Ref. 9. Dedicated to the memory of Professor Margaret Avram. (1984)

Muccio, D. D. ; Copan, W. G. ; Abrahamson, W. W. ; [weitere]

Chichester : Wiley-Blackwell

Organic Magnetic Resonance 22 (1984), S. 121-124

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ISSN: 0030-4921

Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Notizen: The effects of protonation and hydrogen bonding in linear Schiff bases obtained from n-butylamine with butyraldehyde, crotonaldehyde, sorbaldehyde and all-trans-retinal were studied by means of 15N and 13C NMR. The protonation-induced chemical shifts (Δδ) are an order of magnitude larger for 15N than for 13C. For 15N, this effect was found to increase with the extent of conjugation, culminating in the retinylideneimine (Δδ = -146 ppm), which constitutes a model for the study of the structure of the Schiff base linkage in visual pigments and related systems. Theoretical calculations of protonation-induced Δδ values based on MINDO/ 3 are in agreement with experimental results.

Zusätzliches Material: 4 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/mrc.1270220214

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8

Digitale Medien

NQR investigations of 35Cl in crystalline 2,5-dichloro-4-nitroaniline (1981)

Ramanamurti, D. V. ; Srihari, D. ; Venkatacharyulu, P. ; [weitere]

Chichester : Wiley-Blackwell

Organic Magnetic Resonance 16 (1981), S. 123-125

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ISSN: 0030-4921

Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Notizen: Two NQR lines were observed for 35Cl in 2,5-dichloro-4-nitroaniline at room temperature, using a self-quenched super-regenerative spectrometer. Analysis of the Zeeman effect on the two lines using a cylindrical single crystal reveals that the crystal belongs to the monoclinic system. The principal field gradient Z axes enclose an angle of 35° and 28° in the cases of the low and high frequency resonance lines, respectively. The b axis is parallel to 81°, 280°. The unit cell contains either two or a multiple of two molecules. The molecules in the crystal can be arranged into two sets, with the molecular planes in each set being parallel among each other. The angle subtended between the two planes is 159° and each of the planes is inclined to the b axis at an angle of 79.5°. There is an in-plane bending of the two C—Cl bonds by 5.5°. The ionic, single bond and double bond characters of the C—Cl bonds for both chlorines are almost equal, and are in the ratio 24:73:3.

Zusätzliches Material: 3 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/mrc.1270160212

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9

Digitale Medien

Vibrational spectra and normal coordinate analysis of CF3OF and CF3OCl (1980)

Kuo, J. C. ; Desmarteau, D. D. ; Fateley, W. G. ; [weitere]

Chichester [u.a.] : Wiley-Blackwell

Journal of Raman Spectroscopy 9 (1980), S. 230-238

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ISSN: 0377-0486

Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie , Physik

Notizen: The IR spectra (1400 cm-1 to 160 cm-1) of the gases at ambient temperature and the Raman spectra (below 1400 cm-1) of the liquids near -196°C are reported for CF3OF and CF3OCl. All fundamentals are assigned under Cs symmetry and the results of a normal coordinate analysis are presented. The assignments of Smardzewski and Fox are adopted with one exception for both CF3OF and CF3OCl: the CF3 rock of A″ symmetry is assigned near 430 cm-1 and the two bands between 200 cm-1 and 300 cm-1 are assigned to an A′ fundamental, involving CF3 rocking and COX bending and a Δν=2 transition in the CF3 torsion. An extra band at 548 cm-1 in the Raman spectrum of liquid CF3 COl near -196°C is assigned to a CF3OCl⃛Cl2 complex. The values of the force constants d(OX) for CF3OX molecules are suggested to be near those for X2O molecules. More than half the normal modes of A′ symmetry show extensive mixing of symmetry coordinates. In some of these cases the symmetry coordinate for which the normal mode is named is the largest but not the dominant contributor to the potential energy distribution, while in others this symmetry coordinate is not even the largest contributor to the potential energy distribution. No normal modes of A′ symmetry are present in which ν(CO), δs(CF3), δ(COX), or δ(CF3) symmetry coordinates are dominant, and the mode conventionally labeled as v(CO) should be labeled as νs(CF3). For the remaining A′ normal modes and all the A″ normal modes, the symmetry coordinate for which the normal mode is named is dominant in the potential energy distribution.

Zusätzliches Material: 3 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jrs.1250090406

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10

Digitale Medien

Vibrational spectroscopy at high pressures: 44 - thallous carbonate (1983)

Adams, D. M. ; Hatton, P. D. ; Taylor, I. D.

Chichester [u.a.] : Wiley-Blackwell

Journal of Raman Spectroscopy 14 (1983), S. 144-149

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ISSN: 0377-0486

Schlagwort(e): Chemistry ; Analytical Chemistry and Spectroscopy

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie , Physik

Notizen: Raman spectra of thallous carbonate have been studied to c. 52 kbar and mid-IR spectra to 36 kbar. Details of the assignment have also been established using single-crystal IR reflectance and Raman spectroscopy. Phase transitions were found near 13 and 38 kbar on the basis of the Raman evidence, thus supporting the earlier conclusions of Meisalo and Kalliomaki based on x-ray powder and optical results. Analysis of possible space group relations between phases III (ambient) and IV showed that IV must be orthorhombic and adopt one of the D2hn groups, where n = 17, 19, 21, 25 or 28. Structural relationships between the phases are discussed.

Zusätzliches Material: 7 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jrs.1250140304

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