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  • Artikel  (6)
  • Chemistry  (3)
  • Somaclonal variation  (2)
  • Brassica napus  (1)
  • 1985-1989  (6)
  • 1
    Digitale Medien
    Digitale Medien
    Genetic modification of potato development using Ri T-DNA (1985)
    Springer
    Theoretical and applied genetics 70 (1985), S. 440-446 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Agrobacterium rhizogenes ; Potato ; Somaclonal variation ; Genetic manipulation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Forty-two potato plants were regenerated from a hairy-root line obtained after infection of a shoot of Solanum tuberosum cv ‘Desiree’ with Agrobacterium rhizogenes strain LBA 9402 (pRil855). Transformed plants were uniform and had a distinct phenotype and development compared with untransformed controls. Their growth was vigorous, especially early in their development, their roots were abundant and showed reduced geotropism, their leaves were slightly crinkled and glossy and they produced longer tubers with more frequent, prominent eyes. Cytological examination showed that ten of the forty-two transformed plants had either 47 or 49 chromosomes instead of the normal 48. In two of these aneuploids structural changes were observed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00273752
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Genetic manipulation in cultivars of oilseed rape (Brassica napus) using Agrobacterium (1985)
    Springer
    Theoretical and applied genetics 71 (1985), S. 325-329 
    Details
    ISSN: 1432-2242
    Schlagwort(e): A. tumefaciens ; A. rhizogenes ; Brassica napus ; Plant development
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The response of oilseed rape cultivars to infection with Agrobacterium tumefaciens and A. rhizogenes and the possibility of regenerating genetically transformed oilseed rape plants were examined. The frequency at which Agrobacterium induced galls or hairy-roots on in vitro cultured plants ranged from 10% to 70%, depending on the cultivar. From galls induced by the tumorigenic strain T37, known to be strongly shoot inducing on tobacco, roots developed frequently. Occasionally, shoots formed and some of these produced tumour cell specific nopaline. Attempts to grow the transformed shoots into plants have so far been unsuccessful. Whole plants transformed with Ri-T-DNA, however, were regenerated. These had crinkled leaves and abundant, frequently branching roots that showed reduced geotropism, similar to previously isolated Ri T-DNA transformed tobacco and potato plants. The transformed oilseed rape plants flowered, but failed to form seeds.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00252075
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Genetic transformation in two potato cultivars with T-DNA from disarmed Agrobacterium (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 744-750 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Genetic manipulation ; Neomycin phosphotransferase ; Mixed infection ; Somaclonal variation ; Solanum tuberosum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Derivatives of potato (Solanum tuberosum cv.'s ‘Maris Bard’ and ‘Desiree’) transformed with disarmed T-DNA from genetically engineered Agrobacterium tumefaciens strains were isolated. The transformed plants were recovered from shoot-forming tumours induced by infection of wounds with mixedcultures of shoot-inducing A. tumefaciens strains T37 and either Agrobacterium strain LBA1834(pRAL1834), (Hille et al. 1983) or LBA4404(pBIN6; pRAL4404), (Bevan 1984). Two small-scale feasibility experiments gave at least four ‘Maris Bard’ plants transformed with pRAL1834 T-DNA and two ‘Desiree’ plants with pBIN6 T-DNA. The transformed ‘Maris Bard’ plants were morphologically abnormal and highly aneuploid. This was probably an unfortunate side-effect of a tissue culture-step introduced to promote the efficiency of shoot regeneration. The transformed ‘Desiree’ plants, in contrast, were isolated without promoting additional shoot-growth. They were morphologically normal, contained 47 and the euploid 48 chromosomes per cell respectively and had improved growth on media containing kanamycin.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00260785
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Oxygen transfer to mycelial fermentation broths in an airlift fermentor (1987)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 30 (1987), S. 746-753 
    Details
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Oxygen transfer rates and gas holdups were measured in mycelial fermentation broths of Chaetomium cellulolyticum and Neurospora sitophila, each cultured in a 1300-L pilot-plant-scale airlift fermentor. These cultures exhibited highly non-Newtonian flow behavior coupled with a substantial decrease in oxygen transfer rates. The volumetric mass transfer coefficients in these cultures were found to be 65-70% lower than those in water. The data were compared with the available correlations obtained for simulated fermentation broths. In general, the data for C. cellulolyticum are in satisfactory agreement with the correlations for the model media but the data for N. sitophila are higher than that predicted by the correlations. Model media based correlations are found to be applicable to the fermentation processes if the culture medium does not possess a high yield stress.
    Zusätzliches Material: 7 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bit.260300607
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Characterization of copper/enamel interfacial reactions during aging (1988)
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 11 (1988), S. 487-496 
    Details
    ISSN: 0142-2421
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Physik
    Notizen: The interfacial reactions occurring between copper surfaces and a polyesterimide enamel coating during aging at 200°C have been investigated using XPS, AES and SEM. For coating thicknesses of 0.1 μm or greater, a copper oxide layer grows on the copper surface beneath the coating as a result of a reaction with oxygen from the atmosphere. In addition, copper becomes detectable with aging at the air/polyesterimide interface and in the bulk of the coating by surface analysis methods, indicating formation of a mobile copper species. Changes in the XPS line components indicate changes in the polyesterimide structure have occurred. Samples with very thin coatings (c. 100 Å) were also prepared and aged in an attempt to create an interface with dimensions approaching the XPS sampling depth. Upon aging, however, copper oxide grew on top of the original coating, rather than beneath it as in the case of the thicker coatings.
    Zusätzliches Material: 11 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/sia.740110905
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    Characterization of PdCl2/SnCl2 metallization catalysts on a polyetherimide surface by XPS and RBS (1988)
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 11 (1988), S. 160-164 
    Details
    ISSN: 0142-2421
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Physik
    Notizen: A metallization catalyst (based on the PdCl2/SnCl2 system) adsorbed on polyetherimide surfaces was characterized by x-ray photo-electron spectroscopy (XPS), Rutherford back scattering (RBS) and transmission electron microscopy (TEM). The overall Pd/Sn ratio increased from 0.2 to 3.8 when the initial sensitized surface was treated with the accelerator solution. XPS results indicated that before acceleration, only Sn(IV) (stannic hydroxide) is obserable, and after acceleration both metallic Sn and Sn(IV) are present; palladium is present primarily in a metallic form both before and after the accelerator step. Total Pd and Sn coverages were also obtained by RBS, and the total Pd/Sn ratios agree with the XPS surface measurements for specimens just prior to metallization. TEM micrographs showed the adsorbed catalyst consisted of particles 10-50 Å in size covering only a fraction of the surface.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/sia.740110307
    Standort Signatur Erwartet Verfügbarkeit
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