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  • Analytical Chemistry and Spectroscopy  (4)
  • Vestfold Hills  (2)
  • Amino Acid Sequence
  • 1985-1989  (7)
  • 1
    Electronic Resource
    Electronic Resource
    Meromixis in an antarctic fjord: a precursor to meromictic lakes on an isostatically rising coastline (1989)
    Springer
    Hydrobiologia 172 (1989), S. 235-254 
    Details
    ISSN: 1573-5117
    Keywords: antarctic ; fjords ; hypersaline ; lake evolution ; meromixis ; polar lakes ; Vestfold Hills
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Physico-chemical data and isotopic studies (utilising H14CO3, H2 18O, 3H2O) suggest that hypersaline meromixis in Ellis Fjord (Vestfold Hills, Antarctica) was initiated during the middle Holocene period, when hypersaline brine, excluded during the annual formation of sea-ice, gravitated in a density current to the bottom. The application of this contemporary information to the genesis of the meromictic lakes found today in the Vestfold Hills, suggest that their meromixis may have developed prior to isolation from the sea. Comparison of physico-chemical data from the meromictic basins of Ellis Fjord with that of the Vestfold Hills saline lake allows some determination of their evolutionary pathways initiated before, during and after isolation from the sea. Further evolution of each lake can be explained through the individual interaction between climate, the catchment size and basin morphology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00031625
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  • 2
    Electronic Resource
    Electronic Resource
    The annual cycle of heat content and mechanical stability of hypersaline Deep Lake, Vestfold Hills, Antarctica (1988)
    Springer
    Hydrobiologia 165 (1988), S. 115-128 
    Details
    ISSN: 1573-5117
    Keywords: heat budget ; stability ; saline lake ; Vestfold Hills ; Antarctica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Deep Lake, a hypersaline lake of about ten times seawater concentration, rarely freezes and is characterized by a monomictic thermal cycle, Winter circulation, at c. −17 °C, lasts for two to three months. In summer, epilimnetic temperatures from 7–11 °C result in large vertical thermal gradients (21–26 °C) which combine with the enhanced rate of density change per degree Celsius, accompanying such high salt concentration, to produce a particularly stable density configuration in Deep Lake (Schmidt stability c. 8000 g-cm cm−2; 0.785 J cm−2). The Birgean annual heat budget (c. 24500 cal cm−2; 102.7 103 J cm−2) is comparable to that of a temperate lake with a similar mean depth, despite the comparatively high ratio of Birgean wind work to annual heat budget (0.37 g-cm cal−1). Deep lake retains c. 50% of the incident solar radiation during the short summer heating period; within the range estimated for ‘first class’ lakes in North America. Extended daylight hours certainly contribute to the high maximum rate of heating in the lake (444 cal cm−2 day−1; 1.86 103 J cm−2 day−1). Deep Lake cools at a rate less than half its average heating rate. Partitioning the total stability into thermal and saline components shows that salinity can contribute up to c. 20% of the maximum summer Schmidt stability. In early summer, the effect of small melt-streams is to increase stability by diluting the epilimnion. In autumn, evaporative water loss can overtake this effect, creating small de-stabilizing salinity gradients. The usually short-term stabilizing influence of snowfall and drift is less predictable, but is probably more common in winter when strong winds are most frequent. Hypersalinity has a profound effect on the physical cycle of Deep Lake, through freezing point depression and the increased rate of density change with temperature. These changes affect the lake's biota, both in relation to osmotic stress, and by effectively exposing them to a more thermally extreme environment. A comparison between Deep Lake and a smaller lake of similar salinity (Lake Hunazoko, Skarvs Nes), demonstrates that it is inappropriate to consider the biological effects of salinity in isolation. The smaller lake offers warmer epilimnetic conditions for at least part of the summer, which may explain the much greater limnetic algal production in Lake Hunazoko.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00025579
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  • 3
    Electronic Resource
    Electronic Resource
    Negative ion chemical ionization gas chromatography/mass spectrometry of valproic acid metabolites (1989)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 18 (1989), S. 918-926 
    Details
    ISSN: 0887-6134
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A negative ion chemical ionization (NICI) gas chromatographic/mass spectrometric method is described for the identification of 15 valproic acid (VPA) metabolites as their pentafluorobenzyl derivatives. Samples analyzed were serum, urine and saliva taken from a volunteer on VPA at steady state and also given selected doses of (2H6)VPA. Metabolite peaks were identified by comparison to synthetic standards. All the metabolites, like the parent drug, produced abundant [M - 181]- ions, except 3-keto VPA, which gave an [M - 181 - CO2]- ion. Using the NICI method, two new VPA metabolites were identified. One of these metabolites was characterized as 4′-keto-2-ene VPA by synthesis, while the second one appeared to be a positional isomer of 4′-keto-2-ene VPA. The sensitivity of the method was also sufficient to detect metabolites of VPA in saliva. The ratio of the levels of (Z)-2-ene VPA to (E)-2-ene VPA was much greater in saliva than in serum, suggesting stereoselective plasma protein binding or transport of these two metabolites. The lower limit of detection for the quantification of VPA in serum or saliva was 2 ng ml-1.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200181012
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  • 4
    Electronic Resource
    Electronic Resource
    Electron impact induced fragmentations of the 1,4-diene analogues of steroid hormones and related steroids (1985)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 12 (1985), S. 405-408 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The electron impact induced fragmentations observed in the mass spectra of eight 1,4-diene-3-ketopregnane steroids are described. The effect of hydroxyl substituents in positions 11β, 17α and 21, on the typical fragmentation pattern is analysed; in particular the ‘long range’ effect of the 17α-hydroxy group on the relative abundance of the ions m/z 121 and 122 is confirmed using specific deuterium labelling.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200120809
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  • 5
    Electronic Resource
    Electronic Resource
    Preparation and carbon-13 NMR study of 3- and 20-methylene analogues of steroid hormones (1988)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 26 (1988), S. 963-966 
    Details
    ISSN: 0749-1581
    Keywords: 20-Methylenepregnanes ; 3-Methylenepregnanes ; Synthesis ; 13C NMR Steroid hormones ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: 20-Methylpregna-4,20-dien-3-one and the corresponding 17α-hydroxy, 20-hydroxymethyl and 17α-hydroxy-20-hydroxymethyl derivatives were prepared from the 20-keto compounds by Wittig reaction or a modified Simmons-Smith reaction. The 13C NMR spectra of these steroids and of 3-methylene derivatives are analysed. Solvent effects produced by addition of methanol-d4 in the spectra of the 20-methylene steroids are correlated with intramolecular hydrogen bonding in the corresponding 20-ketosteroids.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260261105
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  • 6
    Electronic Resource
    Electronic Resource
    13C NMR spectra of substituted indoles (1986)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 24 (1986), S. 829-831 
    Details
    ISSN: 0749-1581
    Keywords: Indole ; indole derivatives ; 13C NMR spectra ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: 13C chemical shifts for indole and ten indoles substituted at positions 1, 3 and/or 6 are reported. Previous assignments have been revised using specific deuterium labelling and selective proton decoupling.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260240919
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  • 7
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    Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda (1989)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1989-12-08
    Description: A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire. The system allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation. It was possible to generate, in 2 weeks, large numbers of monoclonal Fab fragments against a transition state analog hapten. The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Huse, W D -- Sastry, L -- Iverson, S A -- Kang, A S -- Alting-Mees, M -- Burton, D R -- Benkovic, S J -- Lerner, R A -- New York, N.Y. -- Science. 1989 Dec 8;246(4935):1275-81.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2531466" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Animals ; Antibodies, Monoclonal/*biosynthesis/genetics ; Antibody Specificity ; Antigen-Antibody Reactions ; Bacteriophage lambda/*genetics ; Base Sequence ; Cloning, Molecular/methods ; Escherichia coli/genetics ; Gene Amplification ; Gene Library ; *Genetic Vectors ; Hemocyanin/analogs & derivatives/immunology ; Immunoglobulin Fab Fragments/biosynthesis ; Immunoglobulin Fragments/*biosynthesis/genetics ; Mice ; Molecular Sequence Data ; Organophosphorus Compounds/immunology ; Recombinant Proteins/biosynthesis/genetics
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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