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  • Agrobacterium rhizogenes  (2)
  • A. tumefaciens  (1)
  • Crown gall  (1)
  • Karmarkar's algorithm  (1)
  • Springer  (5)
  • 1985-1989  (5)
Collection
Keywords
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  • Springer  (5)
Years
  • 1985-1989  (5)
Year
  • 1
    Electronic Resource
    Electronic Resource
    An extension of Karmarkar's algorithm for linear programming using dual variables (1986)
    Springer
    Algorithmica 1 (1986), S. 409-424 
    Details
    ISSN: 1432-0541
    Keywords: Linear programming ; Karmarkar's algorithm ; Duality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Mathematics
    Notes: Abstract We describe an extension of Karmarkar's algorithm for linear programming that handles problems with unknown optimal value and generates primal and dual solutions with objective values converging to the common optimal primal and dual value. We also describe an implementation for the dense case and show how extreme point solutions can be obtained naturally, with little extra computation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01840455
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  • 2
    Electronic Resource
    Electronic Resource
    Genetic modification of potato development using Ri T-DNA (1985)
    Springer
    Theoretical and applied genetics 70 (1985), S. 440-446 
    Details
    ISSN: 1432-2242
    Keywords: Agrobacterium rhizogenes ; Potato ; Somaclonal variation ; Genetic manipulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Forty-two potato plants were regenerated from a hairy-root line obtained after infection of a shoot of Solanum tuberosum cv ‘Desiree’ with Agrobacterium rhizogenes strain LBA 9402 (pRil855). Transformed plants were uniform and had a distinct phenotype and development compared with untransformed controls. Their growth was vigorous, especially early in their development, their roots were abundant and showed reduced geotropism, their leaves were slightly crinkled and glossy and they produced longer tubers with more frequent, prominent eyes. Cytological examination showed that ten of the forty-two transformed plants had either 47 or 49 chromosomes instead of the normal 48. In two of these aneuploids structural changes were observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273752
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  • 3
    Electronic Resource
    Electronic Resource
    Genetic manipulation in cultivars of oilseed rape (Brassica napus) using Agrobacterium (1985)
    Springer
    Theoretical and applied genetics 71 (1985), S. 325-329 
    Details
    ISSN: 1432-2242
    Keywords: A. tumefaciens ; A. rhizogenes ; Brassica napus ; Plant development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The response of oilseed rape cultivars to infection with Agrobacterium tumefaciens and A. rhizogenes and the possibility of regenerating genetically transformed oilseed rape plants were examined. The frequency at which Agrobacterium induced galls or hairy-roots on in vitro cultured plants ranged from 10% to 70%, depending on the cultivar. From galls induced by the tumorigenic strain T37, known to be strongly shoot inducing on tobacco, roots developed frequently. Occasionally, shoots formed and some of these produced tumour cell specific nopaline. Attempts to grow the transformed shoots into plants have so far been unsuccessful. Whole plants transformed with Ri-T-DNA, however, were regenerated. These had crinkled leaves and abundant, frequently branching roots that showed reduced geotropism, similar to previously isolated Ri T-DNA transformed tobacco and potato plants. The transformed oilseed rape plants flowered, but failed to form seeds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00252075
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  • 4
    Electronic Resource
    Electronic Resource
    Changes in translatable poly(A) RNA from differentiated potato tissues transformed with shoot-inducing Ti TL-DNA of Agrobacterium tumefaciens (1986)
    Springer
    Plant molecular biology 6 (1986), S. 213-220 
    Details
    ISSN: 1573-5028
    Keywords: Crown gall ; cytokinins ; in vitro translation ; poly(A) RNA ; potato ; T-DNa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two dimensional gel electrophoresis was used to examine differences in steady state total poly(A) RNA from untransformed potato (Solanum tuberosum cv. Maris Bard) and potato transformed with shoot-inducing TL-DNA from A. tumefaciens. RNA was compared from phenotypically very distinct in vitro cultured shoots, more similar grafted plants and tubers. In each case between 200–400 translation products were identified representing the more abundant poly(A) mRNA's. In general, poly(A) RNA from the transformed tissues gave more high molecular weight products. This increase was most evident in poly(A) RNA from shoot cultures. Depending on the tissue examined, 1–5% of the translation products with a molecular weight 〈43 KD were observed to increase or decrease in abundance. The influence of T-DNA on cellular gene expression in the different transformed potato tissues is discussed in relation to previously determined changes in T-DNA gene expression (particularly of the T-DNA cytokinin gene) and the corresponding changes in endogenous hormone concentrations. It is concluded that some of the specific changes in low molecular weight products are either directly caused by the increased cytokinin levels or are indirectly involved in maintaining the transformed phenotype. re]19850530 rv]19851206 ac]19851210
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015227
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  • 5
    Electronic Resource
    Electronic Resource
    Developmental regulation of RI TL-DNA gene expression in roots, shoots and tubers of transformed potato (Solanum tuberosum cv. Desiree) (1986)
    Springer
    Plant molecular biology 6 (1986), S. 321-330 
    Details
    ISSN: 1573-5028
    Keywords: Agrobacterium rhizogenes ; Solanum tuberosum ; T-DNA ; gene expression ; tuberisation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Expression of TL-DNA from Agrobacterium rhizogenes plasmid pRi 1855 was examined in a transformed derivative of Solanum tuberosum cv. Desiree, D9X8a. Northern blot analysis identified at least nine TL-DNA coded transcripts in roots, shoots and tubers but their relative abundance differed within and between organs. This revealed a distinctive pattern of organ specified differential expression. Grafting experiments showed that the abnormal shape of tubers of transformed potato was probably determined by TL-DNA products synthesised within the tuber and not by diffusable products synthesised in other parts of the plant. The abundance of at least one transcript, tr5, was probably determined by culture conditions. Implications for functions and control of expression of Ri TL-DNA genes are discussed. It is suggested that Ri TL-DNA provides a convenient and extensive set of model genes to study variation and stability of expression of linked foreign genes introduced into plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00034939
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