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  • Articles  (8)
  • Springer  (8)
  • 1985-1989  (7)
  • 1965-1969  (1)
  • Biology  (8)
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  • Articles  (8)
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  • 1
    Electronic Resource
    Electronic Resource
    Lipoide als zytochemisches Substrat der Bindungsorte basischer Vitalfarbstoffe in Blut-, Epithel- und Tumorzellen (1967)
    Springer
    Histochemistry and cell biology 10 (1967), S. 309-320 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die zytochemische Natur der Anreicherungsorte basischer Vitalfarbstoffe (Akridinorange, Nilblausulfat und Neutralrot) im Zytoplasma von Blutplättchen, Leukozyten, Mäuseaszites-Tumorzellen und Epithelzellen wurde untersucht. Dabei stellte sich heraus, daß bei allen untersuchten Zelltypen Phospholipoide oder Phospholipoproteide als Substrat der umschriebenen Farbstoffbindung in lysosomalen Zellstrukturen dienen. Die Supravitalfärbung mit basischen Farbstoffen ist demnach als zytochemischer Lipoidnachweis geeignet, wenn unter bestimmten Bedingungen und unter Einhaltung eines definierten Färbestadiums (Stadium der granulären oder vakuolären Farbstoffverteilung) beobachtet wird. Auf die methodischen Vorteile der Supravitalfärbung wird hingewiesen.
    Notes: Summary The cytochemical character of the substrat for basic vital dyes (acridine orange, nil blue sulfate, neutral red) has been studied in the cytoplasma of blood platelets, leucocytes, Ehrlich Ascites tumor cells and epithelial cells. It could be demonstrated that the vital dyes are bound to lysosomal phospholipids or phospholipoproteins in the different cell types. Supravital staining with basic dyes is therefore considered to be a useful method for identifying lipid material in cytology, provided defined conditions and stades of staining are observed. The advantages of the proposed method are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304313
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  • 2
    Electronic Resource
    Electronic Resource
    Changes in glycoconjugates in rat submandibular gland after chronic treatment with reserpine and isoproterenol (1988)
    Springer
    Histochemistry and cell biology 90 (1988), S. 285-288 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Chronic treatment of rats with reserpine, isoproterenol, or a combination of these two agents has been suggested as a means to produce an experimental animal model for the chronic exocrinopathy cystic fibrosis. The effect of these treatments on glycoconjugate distribution in rat submandibular gland acinar cells was investigated by quantitative lectin cytochemistry. Significant changes in wheat-germ agglutinin (WGA), soy bean agglutinin (SBA) and concanavalin A (Con A) binding sites in the mucus granules were observed, but peanut agglutinin (PNA) binding was not significantly affected. The quantitative changes in glycoconjugates in the acinar cells of the submandibular gland could be a possible explanation for the increased binding of calcium by the intracellular mucus noted in previous studies on these animal models.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495972
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  • 3
    Electronic Resource
    Electronic Resource
    Fluorescence of 3-keto-steroids in aqueous solution (1986)
    Springer
    European biophysics journal 14 (1986), S. 29-35 
    Details
    ISSN: 1432-1017
    Keywords: Fluorescent steroid probes ; steroid-protein interactions, energy alternation of n − π * and π − π * states (level crossing)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract The physiologically important 3-keto-steroids are non-fluorescent or only weakly fluorescent in protic as well as in aprotic solvents. In contrast, the 4,6,8(14)-triene-3-one steroids are highly fluorescent in aqueous solution but they do not appreciably fluoresce in other solvents. Evidence is presented that the introduction of double bonds into the skeleton of the 3-keto-steroids leads to a decrease of the energy of the lowest π − π * state, bringing this level into the neighbourhood of the non-fluorescent n − π * state. As a consequence, for two states of approximately the same energy, relatively small perturbations such as those due to solvent interactions, protein binding and micelle formation, will then determine whether a system will fluoresce (π − π * state lowest) or not (n − π * state lowest). When the fluorescent 3-keto-steroids, having three conjugated double bonds, bind to proteins, the fluorescence intensity becomes almost zero, making these compounds useful as probes for steroid-protein interactions. This quenching of the fluorescence is explained by a decrease in energy of the n − π * state relative to the π − π * state of the steroids due to hydrophobic interactions with the proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260400
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  • 4
    Electronic Resource
    Electronic Resource
    Alterations in cerebrospinal fluid uridine, hypoxanthine, and xanthine in head-injured patients (1988)
    Springer
    Cellular and molecular neurobiology 8 (1988), S. 235-243 
    Details
    ISSN: 1573-6830
    Keywords: head injury ; uridine ; hypoxanthine ; xanthine ; ventriculostomy ; hydrocephalus ; lumbar spinal fluid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. Examination of the cerebrospinal fluid (CSF) of head-injured patients reveals that the concentration of intraventricular xanthine is elevated and that of uridine is decreased relative to those of adult lumbar CSF. 2. No correlations were observed between CSF lactate and CSF hypoxanthine, xanthine, or uridine, suggesting that changes in purine metabolites and the pyrimidine nucleoside do not index similar cellular events as does lactic acid production. 3. Ventricular CSF from hydrocephalic infants had uridine and hypoxanthine concentrations not significantly different from those of normal adult lumbar CSF, but xanthine was significantly elevated. 4. Since uridine has anticonvulsant properties and is a crucial substrate for cerebral metabolism, it may be useful to evaluate this pyrimidine for use in the management of patients with head injury.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00711249
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  • 5
    Electronic Resource
    Electronic Resource
    Altered cytokeratin expression and differentiation induction during neoplastic transformation of cultured rat liver cells by nickel subsulfide (1989)
    Springer
    Cell biology and toxicology 5 (1989), S. 271-286 
    Details
    ISSN: 1573-6822
    Keywords: cell transformation ; cytokeratin ; cytoskeleton ; differentiation ; intermediate filaments ; nickel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Rat liver T51 B cells were maintained in the presence of low concentrations of Ni(H) derived from αNi3S2 for 3–I5 months in culture in order to monitor cytokeratin, differentiation, and transformation patterns. Nickel exposures caused irreversible, heritable juxtanuclear aggregates of cytokeratin CKSS, which increased in size and complexity with prolonged nickel exposure, eventually resembling Mallory bodies and expressing glutamyltransferase. Altered cytokeratin expression was accompanied by induction of differentiation, with markers of both bile ductular cells and hepatocytes, such as induction of cytokeratin polypeptides CK39 and CK49, cell morphology, and cytokeratin filament network changes, whereas control cultures similarly maintained for long periods in culture remained unchanged. Altered cytokeratin expression was also accompanied by acquisition of transformation markers—loss of density dependence, progression toward calcium independence, and (benign) growth in nude mice. Observed cytokeratin aberrations may be a factor in nickel carcinogenesis, in view of the known affinity of the metal for cellular structural proteins, especially keratin, which play a role in maintenance of cell behavior.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01795356
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  • 6
    Electronic Resource
    Electronic Resource
    Phenylacetic acid in an anaerobic swine manure digester (1986)
    Springer
    Journal of industrial microbiology and biotechnology 1 (1986), S. 57-61 
    Details
    ISSN: 1476-5535
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The presence of phenylacetic acid (PAA) in an anaerobic swine manure digester was determined by gas chromatography of the butyl ester and confirmed by mass spectroscopy. PAA concentration increased during start-up of a digester and with low carbon, high nitrogen loading. Unlike acetate, propionate and butyrate, the concentration of PAA varied little through the day in a stable digester loaded once per day. The laboratory scale digester was loaded at 4 g of swine manure solids/liter digester volume per day. The retention time and temperature were 15 days and 37°C. PAA is a microbial intermediate which is produced by one group of anaerobic bacteria and converted to methane by other members of the bacterial community in the digester. As such, it may be a useful indicator of the relative metabolic activity of the bacterial groups and thus of the overall stability of the anaerobic process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01569417
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  • 7
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    Alterations in cerebrospinal fluid uridine, hypoxanthine, and xanthine in head-injured patients (1988)
    Springer
    Details
    Publication Date: 1988-06-01
    Print ISSN: 0272-4340
    Electronic ISSN: 1573-6830
    Topics: Biology
    Published by Springer
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  • 8
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    Altered cytokeratin expression and differentiation induction during neoplastic transformation of cultured rat liver cells by nickel subsulfide (1989)
    Springer
    Details
    Publication Date: 1989-11-01
    Print ISSN: 0742-2091
    Electronic ISSN: 1573-6822
    Topics: Biology , Medicine
    Published by Springer
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