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  • LiClO4  (1)
  • energy transfer  (1)
  • 1990-1994  (2)
  • 1
    ISSN: 1573-5079
    Keywords: Key words ; Photosystem 2 ; oxygen evolution ; Mn cluster ; o-phenanthroline ; LiClO4 ; electron transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We examined the effects of o-phenanthroline and LiClO4 on oxygen evolution and electron transport in the Photosystem 2 complex of the pea. Treatment of Photosystem 2 particles with a combination of 3.0 mM o-phenanthroline and 1.0 M LiClO4 for 30–40 min at 0°C decreased the oxygen-evolving activity with the electron acceptor (either phenyl-p-benzoquinone or 2,6-dichlorophenol indophenol) to less than 5% of the original level. However with the same treatment, the electron-transport activity from an artificial electron donor, 1,5-diphenylcarbohydrazide, to 2,6-dichlorophenol indophenol remained at 60% of the original activity. The amount of manganese in the Photosystem 2 complex decreased in parallel with the loss of oxygen evolution following treatment. These observations suggest that the treatment of the Photosystem 2 complex with o-phenanthroline and LiClO4 inhibits electron transport on the oxygen-evolving side much more significantly than on the electron-acceptor side.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5079
    Keywords: aerobic photosynthetic bacteria ; carotenoid ; energy transfer ; Erythrobacter longus ; fluorescence excitation spectra ; resonance Raman spectra
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In vivo states and functions of carotenoids in the membranes and the isolated RC-B865 pigment-protein complexes from an aerobic photosynthetic bacterium, Erythrobacter longus, are investigated by means of fluorescence excitation and resonance Raman (RR) spectra. Erythroxanthin sulfate, a dominant carotenoid species in the membranes (〉70%), is found not to transfer the absorbed light energy to bacteriochlorophyll (Bchl), and its RR spectra are similar between the in vivo and in vitro states. These observations indicate that erythroxanthin sulfate does not interact with either Bchl or proteins in the membranes, and suggest that its function may be limited to photoprotection by quenching the harmful singlet oxygen. On the other hand, two other carotenoid species contained in the isolated RC-B865 complexes, zeaxanthin and bacteriorubixanthinal, have a high efficiency of energy transfer to Bchl (88±5%). The RR spectra of these two carotenoids, each of which can be selectively obtained by choosing the excitation wavelength, show some characteristics of interactions with proteins or Bchl.
    Type of Medium: Electronic Resource
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