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  • Chemistry  (132)
  • Inorganic Chemistry  (14)
  • Organic Chemistry  (13)
  • Beta vulgaris  (6)
  • Atomic, Molecular and Optical Physics  (5)
  • Magnetism
  • 1990-1994  (143)
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Keywords
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Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Physik (1991)
    Weinheim : Wiley-Blackwell
    Chemie in unserer Zeit 25 (1991), S. viii 
    Details
    ISSN: 0009-2851
    Keywords: Chemistry ; Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ciuz.19910250212
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular characterization of mitochondrial DNA of different subtypes of male-sterile cytoplasms of the sugar beet Beta vulgaris L. (1991)
    Springer
    Theoretical and applied genetics 82 (1991), S. 11-16 
    Details
    ISSN: 1432-2242
    Keywords: Beta vulgaris ; Cytoplasmic male sterility ; mtDNA ; Restriction fragment patterns ; Filter hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitochondrial (mt) DNA from eight cytoplasmic male-sterile (cms) lines of sugar beet from different breeding stations was investigated by restriction fragment analysis and Southern hybridization. All cms lines showed similar but not identical restriction and hybridization signal patterns, readily distinguishable from those of fertile (N) cytoplasm. Digestion of the mtDNA with BamHI, EcoRI, SalI, and XhoI revealed distinct differences between the sterile lines, and six subtypes of the S cytoplasm could be distinguished. Differences between the sterile lines were confirmed by hybridization with a $$\overline{\overline {COX}} II$$ gene probe revealing minor, line-specific hybridization signals. The data presented provide evidence for the existence of considerable variation within the only commercially used source of cms in the sugar beet, the Owen's type of cytoplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231271
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  • 3
    Electronic Resource
    Electronic Resource
    Rapid hybridization-based assays for identification by DNA probes of male-sterile and male-fertile cytoplasms of the sugar beet Beta vulgaris L. (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 819-824 
    Details
    ISSN: 1432-2242
    Keywords: Beta vulgaris ; Cytoplasmic male sterility ; mtDNA probes ; Miniassays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Methods are described whereby hybridization of mitochondrial (mt) DNA with different DNA probes can definitely distinguish male-fertile and and male-sterile (cms) cytoplasms of sugar beet Beta vulgaris L. We have developed two types of miniassays. (1) Comparative methods requiring the isolation and restriction of total cellular DNA, hybridization with cloned mtDNA fragments from either fertile or male-sterile cytoplasms, and comparison of the hybridization patterns to the fertile-and sterile-specific patterns of mtDNA of sugar beet for the given mtDNA probe. For these analyses, we routinely used 1 g of plant material to determine the type of cytoplasm. (2) Noncomparative (“plus-minus”) methods requiring neither the isolation of pure DNA nor restriction, electrophoresis, or Southern blotting. Instead, alkaline-SDS plant extracts from as little as 50 mg of plant material were dot-blotted and hybridized with fertile-specific (mitochondrial minicircular DNA) and/or cms-specific probes (consisting of a 2.3-kb mtDNA sequence exclusively occurring in the cms cytoplasm). The assays are simple to perform, give definitive results, are nonde-structive to the plants, and may be used in mass screening of sugar beet populations for hybrid production or in in vitro culture processes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224996
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  • 4
    Electronic Resource
    Electronic Resource
    Isozyme analysis of primary trisomies in beet (Beta vulgaris L.). Genetical characterization and techniques for chromosomal assignment of two enzyme-coding loci: leucine aminopeptidase and glutamate oxaloacetate transaminase (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 761-768 
    Details
    ISSN: 1432-2242
    Keywords: Primary trisomies ; Beet ; Beta vulgaris ; Isozyme polymorphism ; Chromosomal assignment ; Distorted segregation ; Dosage shift
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Segregating families of beet (Beta vulgaris) were used to verify the monofactorial inheritance of two enzyme-coding loci, leucine aminopeptidase (Lap1) and glutamate oxaloacetate transaminase (Got3). With a series of primary trisomies and using three methods to discriminate between the critical trisomic (the locus is situated on the triplicated chromosome) and the non-critical ones, it was possible to allocate the two loci to beet chromosomes I and II, respectively. For the locus Lap1 distorted segregation ratios were estimated, and the incorporation of three alleles into one plant was attempted. In the case of Got3 the measurement of the allele dosage effect after electrophoresis was chosen as the major strategy. The output of laser densitometric scans were subjected to the non-parametrical Wilcoxon-Mann-Whitney test.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222667
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  • 5
    Electronic Resource
    Electronic Resource
    Isolation of DNA markers linked to a beet cyst nematode resistance locus in Beta patellaris and Beta procumbens (1992)
    Springer
    Molecular genetics and genomics 235 (1992), S. 432-440 
    Details
    ISSN: 1617-4623
    Keywords: Heterodera schachtii ; Beta vulgaris ; Monosomic additions ; Molecular markers ; Disease resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In cultivated beet no useful level of resistance of the beet cyst nematode (BCN) Heterodera schachtii Schm. has been found, unlike the situation in wild species of the section Procumbentes. Stable introgression of resistance genes from the wild species into Beta vulgaris has not been achieved, but resistant monosomic additions (2n =18 + 1), diploids of B. vulgaris with an extra alien chromosome carrying the resistance locus, have been obtained. Here we describe a new series of resistant monosomic fragment addition material of B. patellaris chromosome 1 (pat-1). We further describe the cloning of a single-copy DNA marker that specifically hybridizes with a monosomic addition fragment of approximately 8 Mb (AN5-90) carrying the BCN resistance locus. This marker and another fragment-specific, single-copy DNA marker probably flank the BCN locus on the addition fragment present in the AN5-203 material, which is approximately 19 Mb in size. Furthermore, several specific repetitive DNA markers have been isolated, one of which hybridizes to AN5-90 and also to DNA from a smaller DNA segment of Beta procumbens, present in line B883, carrying a BCN resistance locus introgressed into the B. vulgaris genome. This suggests that the specific repetitive marker is closely linked to the BCN locus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00279390
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  • 6
    Electronic Resource
    Electronic Resource
    Expression in vitro of resistance to Heterodera schachtii in hairy roots of an alien monotelosomic addition plant of Beta vulgaris, transformed by Agrobacterium rhizogenes (1990)
    Springer
    Euphytica 48 (1990), S. 153-157 
    Details
    ISSN: 1573-5060
    Keywords: Beta vulgaris ; Beta patellaris ; Agrobacterium rhizogenes ; beet cyst nematode ; Heterodera schachtii ; nematode resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Hairy roots, induced by Agrobacterium rhizogenes, were obtained of nematode susceptible beet plants (Beta vulgaris) and of the nematode resistant alien monotelosomic addition AN5, carrying a telosome from B. patellaris. The additional telosome was found to be stably present in vitro in the roots of AN5. The hairy root cultures were inoculated with larvae of the beet cyst nematode Heterodera schachtii. On the root culture of AN5 significantly less cysts developed than on the other root cultures. These results indicate that the resistance to the beet cyst nematode is expressed in the roots after transformation and can be monitored under in vitro conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037194
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  • 7
    Electronic Resource
    Electronic Resource
    Natural variation within the genus Beta and its possible use for breeding sugar beet: A review (1990)
    Springer
    Euphytica 49 (1990), S. 57-76 
    Details
    ISSN: 1573-5060
    Keywords: breeding ; Beta vulgaris ; sugar beet ; natural variation ; wild Beta species ; disease resistances
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Sugar beet is a relatively young crop, which supposedly has a narrow genetic base. Natural variation occurring in primitive beet varieties and in wild Beta species has been used for breeding sugar beet. This paper reviews information on desirable characteristics in Beta germplasm and the attempts made for the introgression of such characters into commercial breeding material. After an introduction on the availability of germplasm and the possibilities of hybridisation, attention is focussed on the mating system (especially male sterility), on morphological and physiological characteristics, including yield and sugar content, and on resistances to diseases and pests.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024131
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  • 8
    Electronic Resource
    Electronic Resource
    Size and shape parameter of the actin-binding protein profilin in solution: A depolarized and polarized dynamic light scattering study (1990)
    New York : Wiley-Blackwell
    Biopolymers 30 (1990), S. 219-222 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.360300123
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  • 9
    Electronic Resource
    Electronic Resource
    Restrained energy refinement with two different algorithms and force fields of the structure of the α-amylase inhibitor tendamistat determined by nmr in solution (1990)
    New York : Wiley-Blackwell
    Biopolymers 29 (1990), S. 695-706 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The solution structure of an α-amylase inhibitor, tendamistat, calculated from nmr data with the distance geometry program DISMAN is subjected to restrained energy minimization. To study the influence of force field parametrizations and the convergence behavior of refinement algorithms, two different programs were used. AMBER is an established software package including a steepest descent and/or conjugent gradient optimizer in the Euclidian space; the name AMBER also represents a force field. The program FANTOM (fast Newton-Raphson torsion angle energy minimizer) is a new restrained energy refinement implementation of the Newton-Raphson algorithm, which uses second derivatives of the conformational energy in dihedral angle space with the ECEPP/2 force field. For both programs the normal energy force field was supplemented with an additional potential of the form ΣA(di - ui)6 (if di 〉 ui), which enforces upper limits ui to selected distances di as measured by nmr. Improvements of the intramolecular interactions with a decrease of the internal energies of about 1000 kcal/mol could be achieved without increasing the distance constraint violations. The restrained energy refinements caused only small changes of the molecular geometries: The root mean square distance values for the backbone atoms between the initial DISMAN structure and the refined structures are about 0.5 Å for AMBER and about 0.7 Å for FANTOM. Local conformational changes during the restrained energy minimizations are analyzed with respect to hydrogen-bond formation, and with respect to comparisons of the solution structure and the crystal structure.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.360290404
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  • 10
    Electronic Resource
    Electronic Resource
    Internal dynamics of tRNAPhe studied by depolarized dynamic light scattering (1990)
    New York : Wiley-Blackwell
    Biopolymers 30 (1990), S. 975-983 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The collective internal dynamics of transfer RNAphe from brewer's yeast in solution was studied by depolarized dynamic light scattering (DDLS). Within the melting region of tRNA the depolarized spectra consist of two Lorentzian, where the narrow (slow) component describes the overall rotation of the macromolecule. The broad component is attributed to the collective reorientation of the bases within the biopolymer. At high temperature only this relaxation process is observed in the spectrum. The viscosity dependence of the collective internal relaxation process is described by the Stokes-Einstein-Debye equation for rotational diffusion. Estimates of the internal orientational pair correlation factor from the integral depolarized intensities of tRNAPhe solutions indicates that the observed dynamics correspond to the collective reorientation of approximately 5 bases. A comparison of the results presented with DDLS studies on the aggregation of the mononucleotide guanosine-5′-monophosphate confirms this result. For a further characterization of the relaxation process we studied the effect of hydrostatic pressure (1-1000 bar) on the depolarized spectra of tRNA. While other spectroscopic methods like nmr, fluorescence polarization anisotropy decay, or ESR give information about the very local motion of a single base within the DNA or RNA, this study shows that by DDLS one can characterize collective internal motions of macromolecules.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.360300912
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