Summary
In cultivated beet no useful level of resistance of the beet cyst nematode (BCN) Heterodera schachtii Schm. has been found, unlike the situation in wild species of the section Procumbentes. Stable introgression of resistance genes from the wild species into Beta vulgaris has not been achieved, but resistant monosomic additions (2n =18 + 1), diploids of B. vulgaris with an extra alien chromosome carrying the resistance locus, have been obtained. Here we describe a new series of resistant monosomic fragment addition material of B. patellaris chromosome 1 (pat-1). We further describe the cloning of a single-copy DNA marker that specifically hybridizes with a monosomic addition fragment of approximately 8 Mb (AN5-90) carrying the BCN resistance locus. This marker and another fragment-specific, single-copy DNA marker probably flank the BCN locus on the addition fragment present in the AN5-203 material, which is approximately 19 Mb in size. Furthermore, several specific repetitive DNA markers have been isolated, one of which hybridizes to AN5-90 and also to DNA from a smaller DNA segment of Beta procumbens, present in line B883, carrying a BCN resistance locus introgressed into the B. vulgaris genome. This suggests that the specific repetitive marker is closely linked to the BCN locus.
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Allefs JJHM, Salentijn EMJ, Krens FA, Rouwendaal GJA (1990) Optimization of non-radioactive Southern blot hybridization: single copy detection and reuse of blots. Nucleic Acids Res 18:3099–3100
Arumuganathan K, Earle ED (1991) Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9:208–218
Bennett MD, Smith JB (1976) Nuclear DNA amounts of angiosperms. Proc R Soc Lend [Biol] 274:227–274
Brandes A, Jung C, Wricke G (1987) Nematode resistance derived from wild beet and its meiotic stability in sugar beet. Plant Breeding 99:56–64
Coons GH (1975) Interspecific hybrids between Beta vulgaris L. and the wild species of Beta. J Am Soc Sugar Beet Technol 18:281–306
De Jong JH, Speckmann GJ, De Bock ThSM, Van Voorst A (1985) Monosomic additions with resistance to beet cyst nematode obtained from hybrids of Beta vulgaris and wild type Beta species of the section Patellares. II Comparative analysis of the alien chromosome. Z Pflanzenzücht 95:84–94
De Jong JH, Speckmann GJ, De Bock ThSM, Lange W, Van Voorst A (1986) Alien chromosome fragments conditioning resistance to beet cyst nematode in diploid descendants from monosomic additions of Beta procumbens to Beta vulgaris. Can J Genet Cytol 28:439–443
Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 4:19–21
Heijbroek W (1977) Partial resistance of sugarbeet to beet cyst eelworm (Heterodera schachtii Schm.). Euphytica 26:257–262
Heijbroek W, Roelands AJ, De Jong JH (1983) Transfer of resistance to beet cyst nematode from B. patellaris to sugar beet. Euphytica 32:287–298
Heijbroek W, Roelands AJ, De Jong JH, Van Hulst CG, Schoone AHL, Munning RG (1988) Sugar beets homozygous for resistance to beet cyst nematode (Heterodera schachtii Schm.), developed from monosomic additions of B. procumbens to B. vulgaris. Euphytica 38:121–131
Hijner JA (1952) De gevoeligheid van wilde bieten voor bet bietencysteaalltje (Heterodera schachtii). Mededelingen Instituut Rationele Suikerproductie 21:1–10
Jung C, Hermann RG (1991) A DNA probe for rapid screening of sugar beet (B. vulgaris L.) carrying extra chromosomes for wild beets of the Procumbentes section. Plant Breeding 107:275–279
Jung C, Wricke G (1987) Selection of diploid nematode resistant sugar beet from monosomic addition lines. Plant Breeding 98:205–214
Jung C, Wehling P, Löptien H (1986) Electrophoretic investigations on nematode resistant sugar beets. Plant Breeding 97:39–45
Jung C, Kleine M, Fischer F, Herrmann RG (1990) Analysis of DNA from a B. procumbens chromosome fragment in sugar beet carrying a gene for nematode resistance. Theor Appl Genet 79:663–672
Kreike CM, De Koning JRA, Krens FA (1990) Non-radioactive detection of single copy DNA-DNA hybrids. Plant Mol Biol Rep 8:172–179
Lange W, De Bock ThSM, Van Geyt JPC, Oleo M (1988) Monosomic additions in beet (B. vulgaris) carrying extra chromosomes of B. procumbens. II. Effects of alien chromosomes on in vivo and in vitro plant development. Theor Appl Genet 76:656–664
Lange W, Jung C, Heijbroek W (1990a) Transfer of beet cyst nematode resistance from Beta species of the section Patellares to cultivated beet. Proceedings 53rd Winter Congress. International Institute for Sugar Beet Research, Brussels, pp 89–102
Lange W, Oleo M, Wagner H (1990b) Identifizierung von Wildarten — Chromosomen in monosomen Additionstypen von B. vulgaris. Vorträge Pflanzenzücht 18:210–218
Löptien H (1984a) Breeding nematode resistant beets. I. Development of resistant alien additions by crosses between Beta vulgaris L. and wild species of the section Patellares. Z Pflanzenzücht 92:208–220
Löptien H (1984b) Breeding nematode resistant beets. II. Investigations into the inheritance of resistance to Heterodera schachtii Schm in wild species of the section Patellares. Z Pflanzenzücht 93:237–245
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
McGrath JM, Quiros CF, Harada JJ, Landry BS (1990) Identification of Brassica oleracea monosomic alien chromosome addition lines with molecular markers reveals extensive gene duplication. Mol Gen Genet 223:198–204
Mesken M, Lekkerkerker B (1988) Selectie op particle resistentie tegen het bietecystenaaltje in kruisingen van suiker-en voederbieten met B. maritima. Prophyta (Suppl):68–71 (in Dutch)
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Nagamine T, Todd GA, McCann KP, Newbury HJ, Ford-Lloyd BV (1989) Use of restriction length polymorphism to fingerprint beets at the genotype and species levels. Theor Appl Genet 78:847–851
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Savitsky H (1975) Hybridization between Beta vulgaris and B. procumbens and transmission of nematode (Heterodera schachtii) resistance to sugar beet. Can J Genet Cytol 20:177–186
Savitsky H (1978) Nematode (Heterodera schachtii) resistance and meiosis in diploid plants from interspecific Beta vulgaris × Beta patellaris hybrids. Can J Genet Cytol 20:177–186
Savitsky H, Price C (1965) Resistance to sugarbeet nematode (Heterodera schachtii) in F1 tetraploid hybrids between Beta vulgaris and Beta patellaris. J Am Soc Sugar Beet Tech 13:370–373
Schmidt T, Junghans H, Metzlaff M (1990) Construction of B. procumbens specific DNA probes and their application for the screening of B. vulgaris × B. procumbens (2n=19) addition lines. Theor Appl Genet 79:177–181
Speckmann GJ, De Bock ThSM (1982) The production of alien monosomic additions in B. vulgaris as a source for introgression of resistance to the beet cyst nematode (Heterodera schachtii) from Beta species of the section Patellares. Euphytica 31:313–323
Speckmann GJ, De Bock ThSM, De Jong JH (1985) Monosomic additions with resistance to beet cyst nematode obtained from hybrids of Beta vulgaris and wild Beta species of the section Patellares. Z Pflanzenzücht 95:74–83
Straus D, Ausubel FM (1990) Genomic subtraction for cloning DNA corresponding to deletion mutations. Proc Natl Acad Sci USA 87:1889–1893
Sun TP, Goodman HM, Ausubel FM (1992) Cloning the Arabidopsis GA1 locus by genomic subtraction. Plant Cell 4:119–128
Toxopeus H, Lubberts H (1979) Breeding for resistance to the sugar beet nematode (Heterodera schachtii Schm.) in cruciferous crops. Eucarpia Cruciferae 1979 Conference, Wageningen, The Netherlands
Van Geyt JPC, Oleo M, Lange W, De Bock ThSM (1988) Monosomic addition in beet (Beta vulgaris) carrying extra chromosomes of B. procumbens. I. Identification of the alien chromosomes with the help of isozyme markers. Theor Appl Genet 76:577–586
Van Geyt JPC, Lange W, Oleo M, De Bock ThSM (1990) Natural variation within the genus Beta and its possible use for breeding sugar beet: A review. Euphytica 49:57–76
Wagner H, Gimbel EM, Wricke G (1989) Are B. procumbens Chr Sm and Beta webbiana Moq different species. Plant Breeding 102:17–21
Yu MH (1978) Meiotic behaviour of a disomic nematode resistant sugar beet. Crop Sci 18:615–618
Yu MH (1984) Resistance to Heterodera schachtii in Patellares section of the genus Beta. Euphytica 33:633–640
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Salentijn, E.M.J., Sandal, N.N., Lange, W. et al. Isolation of DNA markers linked to a beet cyst nematode resistance locus in Beta patellaris and Beta procumbens . Molec. Gen. Genet. 235, 432–440 (1992). https://doi.org/10.1007/BF00279390
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DOI: https://doi.org/10.1007/BF00279390