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  • SOLID-STATE PHYSICS  (2)
  • Thermophile  (2)
  • Agave tequilana  (1)
  • 1990-1994  (5)
  • 1
    Electronic Resource
    Electronic Resource
    Isolation of auxotrophic and antimetabolite-resistant mutants of the hyperthermophilic bacterium Thermotoga neapolitana (1994)
    Springer
    Archives of microbiology 162 (1994), S. 357-361 
    Details
    ISSN: 1432-072X
    Keywords: Key words     Thermotoga neapolitana ; Thermophile ; Anaerobe ; Auxotrophs ; Antimetabolites
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract      Antimetabolite-resistant and auxotrophic mutants of the hyperthermophilic bacterium Thermotoga neapolitana were isolated to provide strains with genetic backgrounds amenable to genetic analyses. Norleucine, azaleucine, 4-nitropyridine-N-oxide, and 3-amino-1, 2, 4-triazole did not affect growth, while 5-fluorouracil (5 μg/ml), 5-methyltryptophan (250 μg/ml), 6-azauracil (100 μg/ml), and 4-fluorophenylalanine (30 μg/ml) inhibited growth at the indicated minimum inhibitory concentrations. The effect of 5-fluorouracil was analyzed and found to be bacteriostatic. These inhibitors were used to select spontaneously arising resistant mutants. In addition, auxotrophic mutants requiring leucine, tryptophan, adenine, and histidine were isolated following mutagenesis with ethyl methanesulfonate. Six other auxotrophs with undefined growth requirements were also isolated. These strains will be useful for the development of genetic methods for T. neapolitana.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050150
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Isolation of auxotrophic and antimetabolite-resistant mutants of the hyperthermophilic bacterium Thermotoga neapolitana (1994)
    Springer
    Archives of microbiology 162 (1994), S. 357-361 
    Details
    ISSN: 1432-072X
    Keywords: Thermotoga neapolitana ; Thermophile ; Anaerobe ; Auxotrophs ; Antimetabolites
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Antimetabolite-resistant and auxotrophic mutants of the hyperthermophilic bacterium Thermotoga neapolitana were isolated to provide strains with genetic backgrounds amenable to genetic analyses. Norleucine, azaleucine, 4-nitropyridine-N-oxide, and 3-amino-1, 2, 4-triazole did not affect growth, while 5-fluorouracil (5 μg/ml), 5-methyltryptophan (250μg/ml), 6-azauracil (100 μg/ml), and 4-fluorophenylalanine (30 μg/ml) inhibited growth at the indicated minimum inhibitory concentrations. The effect of 5-fluorouracil was analyzed and found to be bacteriostatic. These inhibitors were used to select spontaneously arising resistant mutants. In addition, auxotrophic mutants requiring leucine, tryptophan, adenine, and histidine were isolated following mutagenesis with ethyl methanesulfonate. Six other auxotrophs with undefined growth requirements were also isolated. These strains will be useful for the development of genetic methods for T. neapolitana.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00263784
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Glutamate dehydrogenase activity in normal and vitrified plants of Agave tequilana Weber propagated in vitro (1990)
    Springer
    Plant cell, tissue and organ culture 22 (1990), S. 147-151 
    Details
    ISSN: 1573-5044
    Keywords: Agave tequilana ; gelrite ; glutamate dehydrogenase ; vitrification ; water potential
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Agave tequilana stem explants were used to produce adventitious shoots under a set of different water potentials induced by different concentrations of gelrite in the medium. At high water potentials all shoots were vitrified; as the medium water potential became more negative the degree of vitrification decreased but the number of shoots per explant also diminished. The enzymes NADH and NAD-GDH (EC. 1.4.1.2) were measured along the water potential gradient. GDH activity was high in the non-vitrified tissues and decreased significantly in the vitrified ones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043690
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  • 4
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    Electrochemical characterization of p(+)n and n(+)p diffused InP structures (1993)
    In:  CASI
    Details
    Publication Date: 2013-08-31
    Description: The relatively well documented and widely used electrolytes for characterization and processing of Si and GaAs-related materials and structures by electrochemical methods are of little or no use with InP because the electrolytes presently used either dissolve the surface preferentially at the defect areas or form residual oxides and introduce a large density of surface states. Using an electrolyte which was newly developed for anodic dissolution of InP, and was named the 'FAP' electrolyte, accurate characterization of InP related structures including nature and density of surface states, defect density, and net majority carrier concentration, all as functions of depth was performed. A step-by-step optimization of n(+)p and p(+)n InP structures made by thermal diffusion was done using the electrochemical techniques, and resulted in high performance homojunction InP structures.
    Keywords: SOLID-STATE PHYSICS
    Type: Proceedings of the 12th Space Photovoltaic Research and Technology Conference (SPRAT 12); p 33-42
    Format: application/pdf
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    NASA TECHNICAL REPORTS
  • 5
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    Diffusion length damage coefficient and annealing studies in proton-irradiated InP (1993)
    In:  CASI
    Details
    Publication Date: 2019-07-13
    Description: We report on the measurement of the diffusion length damage coefficient (K(sub L)) and the annealing characteristics of the minority carrier diffusion length (L(sub n)) in Czochralski-grown zinc-doped indium phosphide (InP), with a carrier concentration of 1 x 10(exp l8) cm(exp -3). In measuring K(sub L) irradiations were made with 0.5 MeV protons with fluences ranging from 1 x 10(exp 11) to 3 x 10(exp 13) cm(exp -2). Pre- and post-irradiation electron-beam induced current (EBIC) measurements allowed for the extraction of L(sub n) from which K(sub L) was determined. In studying the annealing characteristics of L(sub n) irradiations were made with 2 MeV protons with fluence of 5 x 10(exp 13) cm(exp -2). Post-irradiation studies of L(sub n) with time at room temperature, and with minority carrier photoinjection and forward-bias injection were carried out. The results showed that recovery under Air Mass Zero (AMO) photoinjection was complete. L(sub n) was also found to recover under forward-bias injection, where recovery was found to depend on the value of the injection current. However, no recovery of L(sub n) after proton irradiation was observed with time at room temperature, in contrast to the behavior of 1 MeV electron-irradiated InP solar cells reported previously.
    Keywords: SOLID-STATE PHYSICS
    Type: NASA-TM-106262 , E-7988 , NAS 1.15:106262 , International Conference on Indium Phosphide and Related Materials; Apr 18, 1993 - Apr 22, 1993; Paris; France
    Format: application/pdf
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    NASA TECHNICAL REPORTS
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