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  • Articles  (247)
  • Springer  (247)
  • 1990-1994  (247)
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  • Articles  (247)
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  • 1
    ISSN: 1432-2099
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2099
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Summary An attempt was made to verify a report (Liboff et al. 1987) that a unique combination of DC and AC magnetic field exposures at room temperature results in a 3-fold increase in45Ca2+ uptake by human lymphocytes in vitro. Exposures at “resonance condition”, as well as at frequencies and amplitudes above and below the reported effective exposure conditions, were without effect, as were exposures at 37° C. Treatment with ionomycin (0.25 µM), a positive control, resulted in a highly significant increase45Ca2+ uptake. Some experiments were performed simultaneously by different investigators. Their results did not differ significantly. All experiments were conducted “double blindly”.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Radiation and environmental biophysics 32 (1993), S. 73-83 
    ISSN: 1432-2099
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract Data on 60-Hz electric field (EF) induced reduction in growth rate of plant roots have strongly supported the hypothesis that the effect is related to an EF-induced transmembrane potential (V i m). An investigation was undertaken to determine if this hypothesis is also applicable to 60-Hz EF-induced reductions in growth rate of mammalian cells in vitro. Human lymphoblastic (RPMI 1788) and human carcinoma (HeLa) cells were selected for study, the former having a relatively small diameter (11.2 μm), and the latter having a relatively large diameter (15.4 μtm). The 60-Hz EFs ranged from 430–1200 V/m in the culture medium. The growth rate of RPMI 1788 cells after 4-days was depressed by about 42% at a 60-Hz EF of 1000–1200 V/m with a response threshold occurring at 950 V/m; theV i m at the response threshold was 8 mV There was no 60-Hz EF-induced effect on HeLa cell growth rate of aV i m of 8 mV (60-Hz EF=700 V/m); a statistically significant effect was achieved atV i m of 11 mV (950 V/m). The data support the hypothesis that above a threshold 60-Hz EF,V i m acts as the initial signal leading to growth rate reductions.
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  • 4
    ISSN: 1432-2056
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To compare the spatial and temporal (seasonal) distribution of dinoflagellates, vertical net hauls were taken along similar cruise tracks in the Scotia Sea, Weddell Sea and across the Polar Front Zone in the austral spring and the austral fall. Sixty-three species of armored dinoflagellates were identified and enumerated. Chisquare and hierarchical cluster analyses were performed to define spatial and seasonal patterns in genera and species assemblages. The dominant genera were Protoperidinium, Dinophysis and Ceratium. The Polar Front Zone was an important biogeographical barrier with Blepharocysta, Gonyaulax, Heteroschisma, Oxytoxum and Podolampas occurring mainly north of the Front. Species found primarily in the austral spring were Ceratium fusus, Ceratium lineatum, Dinophysis antarctica, Dinophysis simplex, Gonyaulax digitale, Protoperidinium pyriforme and Protoperidinium variegatum. Austral fall species included Dinophysis tuberculata and Protoperidinium elegantissum. Distribution of armored dinoflagellates in the Southwestern Atlantic Ocean is influenced at the generic level by spatial considerations, particular with relation to the Polar Front Zone, whereas species composition can be effected by both region and season.
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  • 5
    ISSN: 1432-2242
    Keywords: Transgressive segregation ; Epistasis ; Frequency distribution ; Castle-Wright formula ; Number of effective factors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Gene complementarity among various sources of resistance to greenbug biotype E was assessed. Analysis of the F2 generation of crosses between susceptible and resistant parents (mating 1) and among sources of resistance (mating 2) suggested that resistance in sorghum to greenbug biotype E was complexly inherited and, to some extent, dependent on the nature of both the resistant and susceptible parents. Positive transgressive segregation in the F2 generations of both matings was found to be due to effective plus factors, contributed by both parents in a cross, which complemented each other. The number of plus factors ranged from one to two in the susceptible parents and from two to five in the resistant parents of mating 1, and from one to five in the parents of mating 2. The consistently significant reciprocal effects shown by Sarvasi and PI264453 indicated that these sources had major factors for resistance in their cytoplasms, which were expressed in all their crosses. The results from this study indicated that the sources of resistance complemented each other to give increased number of F2 segregates with increased resistance. Thus, it should be possible to increase and diversify resistance of sorghum to greenbug biotype E by accumulating different, effective plus factors from various sources through recurrent selection.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Machine vision and applications 6 (1993), S. 181-190 
    ISSN: 1432-1769
    Keywords: Real-time image processing ; Image segmentation ; Pixel classification ; VLSI
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract The main focus of this paper is on the architectural and implementation issues of a prototype of a low-level image segmentation architecture (LISA). LISA performs real-time (20 Mpixels/sec) gray-level image segmentation, i.e., assignment of image pixels to a few user-selected classes. A decision-theoretic pattern-recognition approach is used, which is divided into a feature extraction part and a decision analysis part. The feature extraction part is based on extracting local and global descriptions for all of the image pixels. In the decision analysis part we designed a novel no-cross-term classifier, which significantly reduced the hardware complexity. The LISA prototype has been built with custom and off-the-shelf VLSI chips. Some measured results will also be reported.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Pure and applied geophysics 143 (1994), S. 359-385 
    ISSN: 1420-9136
    Keywords: Rock friction ; constitutive laws ; scaling ; characteristic friction distance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract We describe experiments in which large (14×40 cm nominal contact area) blocks of gabbro were sheared in a direct shear apparatus at room temperature, 5 MPa normal stress, and slip velocities from 0.1 to 10 μm/s. The apparatus was servocontrolled using a displacement feedback measurement made directly between the gabbro blocks. Two surface roughnesses were studied (rough, produced by sandblasting, and smooth, produced by lapping with #60 grit) and accumulated displacements reached 60 mm. Measurements of surface topography were used to characterize roughness and asperity dimensions. Step changes in loading velocity were used to interrogate friction constitutive properties. Both rough and smooth surfaces showed appreciable displacement hardening. The coefficient of friction μ for rough surfaces was about 0.45 for initial slip and 0.7 after sliding 50 mm. Smooth surfaces exhibited higher μ and a greater tendency for unstable slip. The velocity dependence of frictiona−b and the characteristic friction distanceD c show systematic variations with accumulated displacement. For rough surfacesa−b started out positive and became negative after about 50 mm displacement andD c increased from 1 to 4 μm over the same interval. For smooth surfaces,a−b began negative and decreased slightly with displacement andD c was about 2 μm, independent of displacement. For displacements 〈30 mm, rough surfaces exhibit a second state variable with characteristic distance about 20 μm. The decrease ina−b with displacement is associated with disappearance of the second state variable. Our data indicate thatD c is controlled by surface roughness in a complex way, including but not limited to the effect of roughness on contact junction dimensions for bare rock surfaces. The data show that simple descriptions of roughness, such as rms and peak-to-trough, are not sufficient to inferD c . Our observations are consistent with a model in whichD c scales with gouge thickness.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 39 (1993), S. 400-404 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Several strains of methane-oxidizing bacteria were isolated and studied to determine their physiological suitability for removal of methane in coal mine atmospheres. One strain, Methylomonas fodinarum ACM 3268, was selected as the most suitable culture for use in the development of a continuous biofilter to be used as a ventilation “air scrubber”. The experimental biofilter utilising a biofilm of M. fodinarum was shown to reduce methane levels substantially provided the residence times were sufficiently long. In the range 0.25–1.0% methane in air, commonly experienced in coal mine atmospheres, more than 70% of the methane was removed with a residence time of 15 min, with a 90% reduction at 20 min. Even at a residence time of 5 min approximately 20% of the methane in air was removed. Equal quantities of O2 are consumed during the bacterial oxidation of methane and 1% methane is converted to 0.7% CO2. Scale-up and alternative biofilter packings are likely to reduce the residence times in the biofilter.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 42 (1994), S. 256-262 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Recording the amount of titrant required to maintain constant pH in a bioreactor where cell metabolism causes acidity changes allows on-line determinations of growth kinetics in computer-controlled batch cultures. A system for making such measurements is described and its performance is investigated. Transient bicarbonate accumulation occurs if the culture produces CO2 at high pH values and low gas transfer rates. We have developed a mathematical model for the titrant requirement as a function of the cell growth rate, the gas transfer properties of the bioreactor and the culture pH. According to this model, bicarbonate accumulation affects the stoichiometry between titrant and biomass but does not prevent determination of growth rate constants. These predictions are confirmed using model experiments and measurements during batch growth of microbial cultures.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 42 (1994), S. 256-262 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  Recording the amount of titrant required to maintain constant pH in a bioreactor where cell metabolism causes acidity changes allows on-line determinations of growth kinetics in computer-controlled batch cultures. A system for making such measurements is described and its performance is investigated. Transient bicarbonate accumulation occurs if the culture produces CO2 at high pH values and low gas transfer rates. We have developed a mathematical model for the titrant requirement as a function of the cell growth rate, the gas transfer properties of the bioreactor and the culture pH. According to this model, bicarbonate accumulation affects the stoichiometry between titrant and biomass but does not prevent determination of growth rate constants. These predictions are confirmed using model experiments and measurements during batch growth of microbial cultures.
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