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  • Articles  (27)
  • Springer  (27)
  • 1990-1994  (27)
  • Process Engineering, Biotechnology, Nutrition Technology  (21)
  • Geography  (6)
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  • Articles  (27)
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  • 1
    Electronic Resource
    Electronic Resource
    Development of a transformation system forTrichoderma longibrachiatum and its use for constructing multicopy transformants for theegl1 gene (1994)
    Springer
    Applied microbiology and biotechnology 41 (1994), S. 440-446 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract An efficient transformation system for the fungusTrichoderma longibrachiatum has been developed. Transformation was obtained both by electroporation and polyethyleneglycol treatment, using a plasmid carrying theEscherichia coli hygromycin B phosphotransferase gene as a dominant selectable marker. The transformation frequency was 0.5 to 5 transformants /μg plasmid DNA. Transformation normally occurred by tandem integration of the transforming DNA. A high percentage of the transformants were mitotically unstable. The efficiency of co-transformation was very high (around 90%), and several co-transformants containing multiple copies of theegll gene encoding a β-(1,4)-endoglucanase were obtained. Some of them secrete increased levels of endoglucanase to the culture medium. In addition, theE. coli lacZ gene was expressed in an active form under control of theAspergillus nidulans gpdA gene promoter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00939033
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  • 2
    Electronic Resource
    Electronic Resource
    Development of a transformation system forTrichoderma longibrachiatum and its use for constructing multicopy transformants for theegl1 gene (1994)
    Springer
    Applied microbiology and biotechnology 41 (1994), S. 440-446 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract An efficient transformation system for the fungusTrichoderma longibrachiatum has been developed. Transformation was obtained both by electroporation and polyethyleneglycol treatment, using a plasmid carrying theEscherichia coli hygromycin B phosphotransferase gene as a dominant selectable marker. The transformation frequency was 0.5 to 5 transformants /μg plasmid DNA. Transformation normally occurred by tandem integration of the transforming DNA. A high percentage of the transformants were mitotically unstable. The efficiency of co-transformation was very high (around 90%), and several co-transformants containing multiple copies of theegll gene encoding a β-(1,4)-endoglucanase were obtained. Some of them secrete increased levels of endoglucanase to the culture medium. In addition, theE. coli lacZ gene was expressed in an active form under control of theAspergillus nidulans gpdA gene promoter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01982533
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  • 3
    Electronic Resource
    Electronic Resource
    Development of a transformation system for Trichoderma longibrachiatum and its use for constructing multicopy transformants for the egl1 gene (1994)
    Springer
    Applied microbiology and biotechnology 41 (1994), S. 440-446 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract An efficient transformation system for the fungus Trichoderma longibrachiatum has been developed. Transformation was obtained both by electroporation and polyethyleneglycol treatment, using a plasmid carrying the Escherichia coli hygromycin B phosphotransferase gene as a dominant selectable marker. The transformation frequency was 0.5 to 5 transformants /μg plasmid DNA. Transformation normally occurred by tandem integration of the transforming DNA. A high percentage of the transformants were mitotically unstable. The efficiency of co-transformation was very high (around 90%), and several co-transformants containing multiple copies of the egl1 gene encoding a β-(1,4)-endoglucanase were obtained. Some of them secrete increased levels of endoglucanase to the culture medium. In addition, the E. coli lacZ gene was expressed in an active form under control of the Aspergillus nidulans gpdA gene promoter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01982533
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  • 4
    Electronic Resource
    Electronic Resource
    Cloning, sequence analysis and yeast expression of the egl1 gene from Trichoderma longibrachiatum (1992)
    Springer
    Applied microbiology and biotechnology 38 (1992), S. 370-375 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A gene (egl1) encoding an endoglucanase (EGL1) from Trichoderma longibrachiatum has been cloned and sequenced. This gene, homologous to the T. reesei egl1 gene, differs from it in the length of the introns (particularly the first one) and encoded protein. A cDNA fragment obtained by the rapid amplification of cDNA ends method, which takes advantage of the polymerase chain reaction, has been expressed in yeast under control of the cyc-gal inducible promoter and yeast clones able to secrete active enzyme have been obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00170088
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  • 5
    Electronic Resource
    Electronic Resource
    Airborne pollen and spores of León (Spain) (1993)
    Springer
    International journal of biometeorology 37 (1993), S. 89-95 
    Details
    ISSN: 1432-1254
    Keywords: Airborne pollen ; Airborne spores ; Pollen diagrams ; Meteorological factors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography , Physics
    Notes: Abstract A qualitative and quantitative analysis of airborne pollen and spores was carried out over 2 years (from September 1987 to August 1989) in the city of León. Slides were prepared daily using a volumetric pollen trap, which was placed on the Faculty of Veterinary Science building (University of León) 12m above ground-level. Fifty-one pollen types were observed; the most important of these were: Cupressaceae during the winter,Pinus andQuercus in spring, and Poaceae, Leguminosae and Chenopodiaceae in the summer. The results also showed the existence of a rich mould spore assemblage in the atmosphere. The group of Amerospores (Penicillium, Aspergillus andCladosporium) as well as Dictyospores (Alternaria) were the most abundant;Puccinia was common in the air in August. Fluctuations in the total pollen and spores m3 of air were compared with meteorological parameters (temperature, relative humidity and rainfall). From the daily sampling of the atmosphere of León, considering the maximum and minimum temperature and duration of rainfall, the start of the pollen grain season was observed generally to coincide with a rise in temperature in the absence of rain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01214387
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  • 6
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    Development of a transformation system forTrichoderma longibrachiatum and its use for constructing multicopy transformants for theegl1 gene (1994)
    Springer
    Details
    Publication Date: 1994-06-01
    Print ISSN: 0175-7598
    Electronic ISSN: 1432-0614
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Published by Springer
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    PAPER CURRENT
  • 7
    Electronic Resource
    Electronic Resource
    Effects of different carbon sources on the synthesis of pectinase by Aspergillus niger in submerged and solid state fermentations (1993)
    Springer
    Applied microbiology and biotechnology 39 (1993), S. 36-41 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A study was made to compare the production of pectinase by Aspergillus niger CH4 in solid-state (SSF) and submerged (SmF) fermentations. Production of endo- (endo-p) and exo-pectinase (exo-p) by SSF was not reduced when glucose, sucrose or galacturonic acid (up to 10%) were added to a culture medium containing pectin. Moreover, both activities increased when concentrations of the carbon sources were also increased. In SmF, these activities were strongly decreased when glucose or sucrose (3%) was added to culture medium containing pectin. The addition of galacturonic acid affected endo-p activity production to a lesser extend than exo-p. Final endo-p and exo-p activities in SSF were three and 11 times higher, respectively, than those obtained in SmF. The overall productivities of SSF were 18.8 and 4.9 times higher for endo-p and exo-p, respectively, than those in SmF. These results indicate that regulatory phenomena, such as induction-repression or activation-inhibition, related to pectinase synthesis by A. niger CH4 are different in the two types of fermentation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00166845
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  • 8
    Electronic Resource
    Electronic Resource
    The 1987–88 Alaskan Bight tsunamis: Deep ocean data and model comparisons (1991)
    Springer
    Natural hazards 4 (1991), S. 119-139 
    Details
    ISSN: 1573-0840
    Keywords: Tsunamis ; ocean measurements ; model comparisons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography , Geosciences
    Notes: Abstract Excellent deep ocean records have been obtained of two tsunamis recently generated in the Alaskan Bight on 30 November 1987 and 6 March 1988, providing the best available data set to date for comparison with tsunami generation/propagation models. Simulations have been performed with SWAN, a nonlinear shallow water numerical model, using source terms estimated by a seafloor deformation model based on the rectangular fault plane formalism. The tsunami waveform obtained from the model is quite sensitive to the specific source assumed. Significant differences were found between the computations and observations of the 30 November 1987 tsunami, suggesting inadequate knowledge of the source characteristics. Fair agreement was found between the data and the model for the first few waves of the 6 March 1988 tsunami. Model estimates of the seismic moment and total slip along the fault plane are also in fair agreement with those derived from the published Harvard centroid solution for the 6 March 1988 event, implying that the computed seafloor deformation does bear some similarity to the actual source.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00162783
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  • 9
    Electronic Resource
    Electronic Resource
    Fermentative production of l-lactic acid by Lactobacillus casei DSM 20011 and product recovery using ion exchange resins (1993)
    Springer
    Applied microbiology and biotechnology 40 (1993), S. 23-27 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract In order to produce l(+)-lactic acid to be employed in poly-l-lactic acid polymer production, for biomedical applications, the strain Lactobacillus casei subsp. casei DSM 20011 was studied in a conventional batch mode using different initial concentrations of glucose. The results obtained showed that the initial glucose concentration exerts an influence on the fermentation pattern, modifying the different fermentation parameters. Nevertheless, the product yield remained at a constant value of 0.86 g·g−1. The proposed novel system of product recovery, based on the use of ion-exchange resins, gave high yields of pure lactic acid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00170423
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  • 10
    Electronic Resource
    Electronic Resource
    Expression of mutations and protein release by yeast conditional autolytic mutants in batch and continuous cultures (1993)
    Springer
    Applied microbiology and biotechnology 38 (1993), S. 763-769 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Thermosensitive mutant strains of Saccharomyces cerevisiae that fail to generate an osmotically stable cell wall when grown at a non-permissive temperature release their cell contents upon expression of the mutation. Therefore, they may represent an alternative for the production of homologous or heterologous protein preparations. In order to analyse the expression of two of these mutations, lyt2 and slt2, we grew the corresponding strains under precisely defined conditions in batch and continuous fermentors. A switch in the temperature of batch cultures from 24° C to 37° C determined lysis of the cells with a significant release of intracellular enzymes. These include alkaline phosphatase and periplasmic proteins such as glucan-degrading enzymes, the pattern of cell lysis and protein release being maintained for about 6 h. One-stage continuous cultures of a lyt2 mutant were maintained for long periods at 37° C; a fraction of the population lysed and released the indicated proteins, but eventually a revertant of the lytic phenotype was selected. To avoid this, a two-stage continuous culture system was developed by connecting two fermentors in series, the effluent from the first one at 24°C being fed to the second one adjusted to 37° C. A steady state of cell lysis and protein liberation was reached in the second-stage fermentor without any evidence of selection of revertants. This system can be very useful for developing conditions for the use of yeast strains to produce protein preparations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00167142
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