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  • biological control  (3)
  • Springer  (3)
  • American Physical Society
  • 1990-1994  (3)
  • 1950-1954
  • 1
    ISSN: 1573-5036
    Keywords: biological control ; cucumber ; Cucumis sativus ; Pythium damping-off ; Pythium nunn ; Pythium ultimum ; Trichoderma harzianum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two biological control agents, Pythium nunn and Trichoderma harzianum isolate T-95, were combined to reduce Pythium damping-off of cucumber in greenhouse experiments lasting 3–4 weeks. T. harzianum T-95, a rhizosphere competent mutant, was applied to seeds and P. nunn was applied to pasteurized and raw soils naturally and artificially infested with Pythium ultimum. Some treatments were also amended with bean leaves to enhance the activity of P. nunn. The biological control of Pythium damping-off was evaluated in a Colorado soil (Nunn sandy loam) and an Oregon soil mix, which were replanted twice after 2 and 3 months. Interactions between P. nunn and T-95 were detected in the Colorado but not the Oregon soil. No consistent evidence of antagonism between P. nunn and T. harzianum was seen, and significant interactions were detected in the Colorado, but not the Oregon soil. In the first planting of some treatments, the combination of P. nunn and T. harzianum gave greater control of damping-off than either applied alone. P. nunn was most effective in soils that were pasteurized or amended with bean leaves. T. harzianum controlled Pythium damping-off in the Colorado, but not the Oregon soil. In both soils, disease declined over time in treatments amended with bean leaves but without P. nunn or T. harzianum added. This suppression was greater in the Colorado soil, which contained an indigenous population of P. nunn. This work demonstrates that two compatible biological control agents can be combined to give additional control of a soil-borne plant pathogen.
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  • 2
    ISSN: 1573-5036
    Keywords: biological control ; cucumber ; Cucumis sativus ; Pythium damping-off ; Pythium nunn ; Pythium ultimum ; Trichoderma harzianum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two biological control agents,Pythium nunn andTrichoderma harzianum isolate T-95, were combined to reduce Pythium damping-off of cucumber in greenhouse experiments lasting 3–4 weeks.T. harzianum T-95, a rhizosphere competent mutant, was applied to seeds andP. nunn was applied to pasteurized and raw soils naturally and artificially infested withPythium ultimum. Some treatments were also amended with bean leaves to enhance the activity ofP. nunn. The biological control of Pythium damping-off was evaluated in a Colorado soil (Nunn sandy loam) and an Oregon soil mix, which were replanted twice after 2 and 3 months. Interactions betweenP. nunn and T-95 were detected in the Colorado but not the Oregon soil. No consistent evidence of antagonism betweenP. nunn andT. harzianum was seen, and significant interactions were detected in the Colorado, but not the Oregon soil. In the first planting of some treatments, the combination ofP. nunn andT. harzianum gave greater control of damping-off than either applied alone.P. nunn was most effective in soils that were pasteurized or amended with bean leaves.T. harzianum controlled Pythium damping-off in the Colorado, but not the Oregon soil. In both soils, disease declined over time in treatments amended with bean leaves but withoutP. nunn orT. harzianum added. This suppression was greater in the Colorado soil, which contained an indigenous population ofP. nunn. This work demonstrates that two compatible biological control agents can be combined to give additional control of a soil-borne plant pathogen.
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  • 3
    ISSN: 1573-8248
    Keywords: Psyllaephagus ; Tamarixia ; Heteropsylla ; biological control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Résumé La fécondité en fonction de l'âge de deux parasitoïdes du psylleHeteropsylla cubana, Psyllaephagus yaseeni etTamarixia leucaenae a été étudiée en condition de laboratoire. A 25 °C,P. yaseeni présente une fécondité plus élevée (R0=192,9) queT. leucaenae (R0=71,2);T. leucaenae montre cependant une sex ratio plus faible (environ 99 % de femelles) queP. yaseeni (environ 50 %). Le taux d'accroissement potentiel (rm=0,236) deT. leucaenae était plus élevé que celui deP. yaseeni (rm=0,188). Les vitesses de développement des parasitoïdes ont été examinées à des températures constantes et fluctuantes et les équations de la vitesse de développement en fonction de la température ont été calculées. A 25 °C, la durée moyenne des générations était respectivement de 28,0 et 18,1 jours pourP. yaseeni etT. leucaenae. Aux températures de 21,5, 25 et 30 °C la durée totale de développement (de l'œuf à l'adulte) était de 28,5, 21,9 et 14,7 jours chezP. yaseeni et 19,2, 12,6 et 9,5 jours chezT. leucaenae. Le taux de parasitisme était faible et la mortalité nymphale était élevée à la température la plus basse, 21,5 °C, pour les deux parasitoïdes. Tous deux présentaient une survie faible à 100 % de HR;P. yaseeni survivait particulièrement bien à une température de 21 °C et à 44 ou 76% d'HR. P. yaseeni attribuait environ 58 % de femelles aux larves de premier stade de développement mais seulement 12 % aux larves de second stade. Environ 99 % de toutes les éclosions deT. leucaenae était constituée de femelles. La taille des femelles issues des larves de 5e stade étaient significativement supérieure à la taille de celles qui émergeaient des larves de 3e ou 4e stade.
    Notes: Abstract Age specific fecundity of two parasitoids,P. yaseeni andT. leucaenae, of the leucaena psyllidH. cubana, were studied under laboratory conditions. At 25 °C,P. yaseeni had a greater fecundity (R0=192.9)_thanT. leucaenae (R0=71.2);T. leucaenae however had a lower sex ratio (about 99 % females) thanP. yaseeni (about 50 % females). Innate capacity for increase (rm=0.236) ofT. leucaenae was higher thanP. yaseeni (rm=0.188). Developmental rates of the parasitoids were examined at constant and fluctuating temperatures and equations of the rate of development against temperature were calculated. At 25 °C, mean generation times were 28.0 and 18.1 days forP. yaeseeni andT. leucaenae respectively. At temperatures of 21.5, 25, and 30 °C total development times (egg to adult) were 28.5, 21.9, and 14.7 days inP. yaseeni and 19.2, 12.6, and 9.5 days inT. leucaenae respectively. The level of parasitism was low and pupal mortality was high at the lower temperature of 21.5 °C for both parasitoids. Both parasitoids showed poor survivorship at 100 % RH,P. yaseeni survived particularly well (32 days) at a temperature of 21.5 °C and 44 or 76 % RH. P. yaseeni allocated about 58 % females to first instar psyllid nymphs but only 12 % females to second instars. About 99 % of allT. leucaenae births were females. Significantly largerT. leucaenae females emerged from fifth instar parasitized nymphs than third or fourth instars.
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