ISSN:
1617-4623
Keywords:
TSaseB
;
Thermal stability
;
Bacillus subtilis
;
DNA sequences
;
Protein sequences
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Bacillus subtilis 168 is known to possess two thymidylate synthase (TSase; EC 2.1.1.45) genes: thyA and thyB. thyB encodes a thermosensitive TSase (inactivated at 46° C) which, in wild-type cells, accounts for only 5–8% of the total cellular TSase activity. In order to investigate the thermal lability of TSaseB we have analyzed the thyB genes of B. subtilis 168 and of an unrelated strain B. subtilis ATCC6633, which is shown here to have a temperature-resistant TSaseB. This conclusion is supported by the frequency of appearance of spontaneous Thy− mutants at 37° C and 46° C, and by the analysis of clones containing the thyB genes from the two strains. The nucleotide sequences of these two thyB genes were compared.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00281594
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