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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 89 (1985), S. 3185-3188 
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 93 (1989), S. 2891-2893 
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 91 (1987), S. 4024-4029 
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 4
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    New York, N.Y. : Periodicals Archive Online (PAO)
    Psychology and Marketing. 9:6 (1992:Nov./Dec.) 453 
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  • 5
    ISSN: 1432-203X
    Keywords: Gossypium hirsutum ; Somatic embryos ; Regeneration ; Morphogenesis ; Genotype
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Seventeen cultivars of cotton (Gossypium hirsutum L.) were evaluated for callus initiation and maintenance using 3 initiation media and 3 maintenance media. After a series of transfers of a 3% glucose media, calli were placed on a 3% sucrose medium. After several weeks calli were observed for the presence of embryo-like structures. Cultivars Coker 201 and Coker 315 were identified as embryogenic. Embryogenic callus has since been routinely obtained within 6 weeks by initiating callus on glucose media for 3–4 weeks followed by transfer to sucrose media. Histological examination has shown that embryos are derived from isodiametric, densely cytoplasmic cells and follow predictable patterns of development. Upon maturity, transfer to auxin-free media with reduced sucrose levels results in embryo germination. Regenerated plants can be transferred to greenhouse within 90 days of callus initiation.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-203X
    Keywords: Cotton ; Gossypium hirsutum L. ; Somatic embryogenesis ; storage proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The storage protein content of somatic embryos of Gossypium hirsutum L. cv. Coker 201 was determined using extinction level, antigen/antibody association detection methods. Mature storage protein was first detected in early globular-stage somatic embryos at a total concentration of 0.36% of the embryo protein mass. Tulip-stage and mature somatic embryos were comprised of 3.0% and 1.3% mature storage protein, respectively. Maximum storage protein synthesis was found to occur during early globular- and early heart-stages. During this period of development, significant levels of protein precursors were found also to accumulate. The pattern of storage protein synthesis, processing and accumulation paralleled the pattern that has been reported for the zygotic system, although somatic embryos accumulate storage protein at much earlier stages and to a lesser degree. The possibility of using complex biochemical pathways to monitor embryogenic systems in vitro is discussed.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 69 (1985), S. 279-284 
    ISSN: 1432-2242
    Keywords: Nuclear-cytoplasmic interaction ; Chlorophyll ; Glycine max L. ; Maternal inheritance ; Plastid ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genetic analysis of a yellow foliar mutant in soybean (Glycine max L. Merr.) showed maternal inheritance of the mutant phenotype designatedcyt-Y 3. The mutant was grown beside normal green sibs (cyt-G 3) under three different photosynthetic photon flux densities (PPFD), and samples were collected to determine pigment content and for electron microscopy analyses of plastid ultrastructure. The plastid ultrastructure ofcyt-Y 3 appeared normal at low PPFD and the carotenoid level ofcyt-Y 3 was also normal, but the chlorophyll content was only approximately one-third that ofcyt-G 3. Under medium and high PPFD,cyt-Y 3 plastids lacked a structured thylakoid, and total chlorophyll content was only 28% and 1% of normal, respectively; the carotenoid levels ofcyt-Y 3 also dropped to 33% and 2% of normal, respectively. These data indicate that the effect of high PPFD oncyt-Y 3 might result from a deficiency in a plastid membrane protein. The resulting changes in membrane configuration could then interfere with the accumulation or stabilization of chlorophylls and carotenoids, thereby resulting in the subsequent photooxidation of both at medium and high PPFD. This mutant could be useful in the study of thylakoid biosynthesis and pigment stabilization, or could provide a source of conditionally identifiable plastids for organelle segregation studies.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-2242
    Keywords: Glycine max ; Glycine tomentella ; Chromosome elimination ; Isoenzyme variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A colchicine-doubled F1 hybrid (2n=118) of a cross between PI 360841 (Glycine max) (2n=40) x PI 378708 (G. tomentella) (2n=78), propagated by shoot cuttings since January 1984, produced approximately 100 F2 seed during October 1988. One-fourth of the F2 plants or their F3 progeny have been analyzed for chromosome number, pollen viability, pubescence tip morphology, seed coat color, and isoenzyme variation. Without exception, all plants evaluated possessed the chromosome number of the G. max parent (2n=40). Most F2 plants demonstrated a high level of fertility, although 2 of 24 plants had low pollen viability and had large numbers of fleshy pods. One F2 plant possessed sharp pubescence tip morphology, whereas all others were blunt-tipped. All evaluated F2 and F3 plants expressed the malate dehydrogenase and diaphorase isoenzyme patterns of the G. max parent and the endopeptidase isoenzyme pattern of the G. tomentella parent. Mobility variants were observed among progeny for the isoenzymes phosphoglucomutase, aconitase, and phosphoglucoisomerase. This study suggests that the G. Tomentella chromosome complement has been eliminated after genetic exchange and/or modification has taken place between the genomes.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 89 (1994), S. 226-232 
    ISSN: 1432-2242
    Keywords: Glycine max ; Phytophthora sojae resistance ; Restriction fragment length polymorphism (RFLP) ; Gene tagging ; Marker-assisted breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Phytophthora root and stem resistance locus Rps1 has been mapped to linkage group N of the USDA-ARS soybean molecular map, approximately 2 cM from locus A071-1. To determine if A071-1 polymorphisms exist that distinguish and tag different Rps1 alleles, germplasms containing the seven Rps1 alleles were screened with eight enzymes for pA071-detectable polymorphisms. Six enzymes revealed at least one polymorphic fragment. All six detected a polymorphism at A071-1 as determined by restriction fragment length polymorphism mapping, comparison to an EMBL3 clone containing locus A071-1, and Southern hybridization with probes specific for locus A071-1. Screening of the Rps1 donors and 24 Rps1-and 15 Rps1-containing U.S. soybean varieties showed that locus A071-1 exhibited three polymorphisms with each enzyme. The polymorphisms detected by one enyme did not always correlate with those detected by the other four, suggesting that multiple mutation events may be responsible for the different A071-1 polymorphisms. Although no combination of alleles distinguished Rps1-and Rps1-containing genotypes, polymorphism at A071-1 made it possible to distinguish five groups of soybean germplasms. Thus, the unusual polymorphism of locus A071-1 should useful for following Rps1 inheritance in many breeding programs.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 79 (1990), S. 465-469 
    ISSN: 1432-2242
    Keywords: Soybean ; RFLP ; Quantitative trait loci ; Germination ; Hard seededness
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Hard seededness in soybean [Glycine max (L.) Merr.] is a quantitative trait that affects the germination rate, viability, and quality of stored seeds. We have used 72 restriction fragment length polymorphisms (RFLPs) to identify genomic regions containing quantitative trait loci (QTL) affecting hard seededness in a segregating population from a G. max by a Glycine soja (Sieb. & Zucc.) cross. Five independent RFLP markers were found to be associated with variation in the hard-seeded trait. These markers and the epistatic interactions between them explain 71% of the variation for hard seededness. A genomic region associated with the i locus accounted for 32% of the variation in this segregating population. This study illustrates one approach to physiological genetic studies in plants.
    Type of Medium: Electronic Resource
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