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  • SPACE RADIATION  (3)
  • Barley  (2)
  • house mouse  (2)
  • 1990-1994  (4)
  • 1985-1989  (3)
  • 1930-1934
  • 1
    ISSN: 1573-4927
    Keywords: arylsulfatase ; chondroitin-4-sulfatase ; electrophoretic variants ; house mouse ; regulation ; sulfatase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Arylsulfatase B (arylsulfate sulfohydrolase; EC 3.1.6.1) activities in C57BL/6J, SWR/J, and A/J mouse liver approximate a 5:3:1 ratio. Each enzyme was purified to apparent homogeneity, and the properties of the three purified enzymes were compared. The purified enzyme behaved as a monomer with an apparent molecular weight of 50,000. The purified enzyme catalyzed the hydrolysis ofp-nitrocatechol sulfate (pNCS), 4-methylumbelliferyl sulfate (4MUS), and chondroitin-4-sulfate (C4S) heptasaccharide. Purified SWR/J arylsulfatase B possessed a higher relative electrophoretic mobility atpH 4.0 than the A/J and C57BL/6J isozymes, and the SWR/J enzyme was more thermostable than either the C57BL/6J or the A/J enzyme. No differences were observed among the three enzymes with respect to their Michaelis constants for 4MUS and pNCS, isoelectric points, responses to inhibitors,pH optima, or electrophoretic mobilities atpH 8.3. The relativein vivo rates of synthesis of C57BL/6J, A/J, and SWR/J arylsulfatase B were comparable.
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  • 2
    ISSN: 1573-4927
    Keywords: arylsulfatase ; chondroitin-4-sulfatase ; electrophoretic variants ; house mouse ; regulation ; sulfatase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Arylsulfatase B (arylsulfate sulfohydrolase; EC 3.1.6.1) activities in C57BL/6J, SWR/J, and A/J mouse liver approximate a 5:3:1 ratio. Each enzyme was purified to apparent homogeneity, and the properties of the three purified enzymes were compared. The purified enzyme behaved as a monomer with an apparent molecular weight of 50,000. The purified enzyme catalyzed the hydrolysis ofp-nitrocatechol sulfate (pNCS), 4-methylumbelliferyl sulfate (4MUS), and chondroitin-4-sulfate (C4S) heptasaccharide. Purified SWR/J arylsulfatase B possessed a higher relative electrophoretic mobility atpH 4.0 than the A/J and C57BL/6J isozymes, and the SWR/J enzyme was more thermostable than either the C57BL/6J or the A/J enzyme. No differences were observed among the three enzymes with respect to their Michaelis constants for 4MUS and pNCS, isoelectric points, responses to inhibitors,pH optima, or electrophoretic mobilities atpH 8.3. The relativein vivo rates of synthesis of C57BL/6J, A/J, and SWR/J arylsulfatase B were comparable.
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  • 3
    ISSN: 1432-2242
    Keywords: Doubled haploids ; Microspore ; Isozymes ; RFLPs ; Barley
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Biochemical, molecular and morphological markers have been used to monitor the segregation of alleles at major gene loci in microspore-derived lines of four spring barley crosses and their parents. Significant deviations from the expected Mendelian ratios were observed for four of the ten markers studied in the cross. Distorted ratios were associated with loci located on chromosomes 4H and 6H. The differential transmission of alleles was in favour of the responsive parent (Blenheim) used in the anther culture studies. For the α-Amy-1 locus on chromosome 6H, the preferential transmission of Blenheim alleles was most pronounced in the haploid regenerants that were colchicine treated. These results are discussed in relation to the genetic control of androgenetic response in barley and with respect to the exploitation of another culture in barley improvement.
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  • 4
    ISSN: 1432-2242
    Keywords: Water-soluble protein (WSP) ; Barley ; β Amylase ; Linkage ; Spring/winter habit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Water-soluble proteins (WSP-2 and WSP-3) and β-amylase (β-AMY-1) were extracted from mature endosperms of 44 spring and 39 winter barley genotypes. The protein and enzyme isoforms were separated in isoelectric focusing gels with a pH gradient of 4–6.5. The Wsp-3 and β-Amy-1 loci were located to chromosomes 4H using the wheat/barley chromosome addition lines. Segregation analysis of F2 and doubled haploid populations showed Wsp-2 and β-Amy-1 to be tightly linked, with a map distance of 11 cMorgans. Isoforms of WSP-2 possessed similar pIs to that of WSP-3 and overlapping bands were observed in the gels. These bands segregated independently in F2 and doubled haploid populations, implying two unlinked genes. All three loci were found to be polymorphic: two alleles were detected at the Wsp-2 locus, three at Wsp-3 and two at β-Amy-1. The frequency of alleles at all three loci was found to be different in winter and spring genotypes. Spring genotypes possessed a wider range of phenotypes than winter genotypes. Spring and winter genotypes could be distinguished on the basis of WSP-3 and β- AMY-1 phenotypes. The linkage between Wsp-3 and β-Amy-1 loci and genes controlling spring/winter habit on chromosome 4H is discussed. It is concluded that Wsp-3 and β-Amy-1 can be used as genetic markers for spring/winter habit in barley genetic research and breeding.
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  • 5
    Publication Date: 2011-08-24
    Description: The Energetic Gamma Ray Experiment Telescope (EGRET) on the Compton Gamma Ray Observatory has observed energetic gamma ray bursts and flares. On May 3, 1991, EGRET detected a gamma ray burst both in the energy measuring NaI (Tl) scintillator and independently in the spark chamber imaging assembly. The NaI spectra were accumulated by a special BURST mode of EGRET. The spectra were measured over a range from 1 to 200 MeV, in three sequential spectra of 1,2, and 4 seconds. During the peak of the burst, six individual gamma rays were detected in the spark chamber, allowing a determination of the burst arrival direction. The intense flares of June were also detected. A solar flare on June 4 was observed to last for several minutes and for a brief time, less than a minute, had significant emission of gamma rays exceeding 150 MeV.
    Keywords: SPACE RADIATION
    Type: In: Gamma-ray bursts; Proceedings of the Workshop, Univ. of Alabama, Huntsville, Oct. 16-18, 1991 (A93-40051 16-93); p. 38-42.
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  • 6
    Publication Date: 2011-08-24
    Description: Intense gamma radiation has been observed from the direction of the quasar 3C 279 throughout the energy range from 30 MeV to over 5 GeV by the Energetic Gamma Ray Experiment Telescope (EGRET) during the period June 15-28, 1991. Its spectrum is well represented by a photon differential power-law exponent of 2.0 +/- 0.1, with a photon intensity above 100 MeV of (2.8 +/- 0.4) x 10 exp -6/sq cm s. For E is greater than 100 MeV, the 2-sigma upper limits were 1.0 x 10 exp -6/sq cm s in 1973 from the SAS 2 observations and 0.3 x 10 exp -6/sq cm s for the combined 1976, 1978, and 1980 COS B observations. Hence, there has been a large increase in high-energy gamma-ray intensity relative to the earlier times, as there has been in the radio, infrared, optical, and X-ray ranges. This source is the most distant and by far the most luminous gamma-ray source yet detected.
    Keywords: SPACE RADIATION
    Type: Astrophysical Journal, Part 2 - Letters (ISSN 0004-637X); 385; L1-L4
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  • 7
    Publication Date: 2019-06-28
    Description: The High Energy Gamma Ray Balloon Instrument was built in part to verify certain subsystems' performance for the Energetic Gamma Ray Experiment Telescope (EGRET) instrument, the high energy telescope to be carried on the Gamma Ray Observatory. This paper describes the instrument, the performance of some subsystems, and some relevant results.
    Keywords: SPACE RADIATION
    Type: OG-9.2-12 , 19th Intern. Cosmic Ray Conf - Vol. 3; p 338-341; NASA-CP-2376-VOL-3
    Format: application/pdf
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