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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 116 (1997), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A salt tolerance experiment was performed to study the effect of the GPert mutation in different genetic backgrounds of spring barley, Hordeum vulgare. L. Twenty-one lines carrying GPert along with 20 related non-GPert lines were grown for 4 weeks in low salt (25 mol m−3 NaCI) and salt stress (175 mol m−3 NaCI) hydroponic cultures. Shoot Na+ content was taken as a measure of salt tolerance. Salt tolerance of the two groups (GPert versus non-GPert) was compared, as was the performance of individual GPert lines with their non-GPert parental lines. The results show that GPert has a general positive effect on salt tolerance in reducing shoot Na+ content, but that this is regulated by genetic background.
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  • 2
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A total of 33 simple sequence repeats (SSRs) was analyzed in 52 genotypes of Hordeum spontaneum originally collected from two different soil types (Terra rossa and Basalt) at Tabigha in Israel. Data on the performance of developmental, morphological, and yield-related traits under well-watered control and water-stress conditions were available from previous experimentation, and powdery mildew susceptibility was scored. Regression analyses based on SSR allele class differences were performed. Highly significant associations were detected at the SSR loci Bmac181 (on chromosome 4H) and Bmac316 (6H) for water -stress tolerance and powdery mildew resistance, respectively. The study shows that association mapping using SSRs and genetically diverse germplasm provides an effective means of relating genotypes to complex quantitative phenotypes.
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 98 (1987), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The main objective of this work is to demonstrate the expression of salt tolerance genes in a wheat-Agropyron amphiploid. Salt tolerance tests were carried out on wheat varieties, ‘Chinese Spring,’ and ‘Glenn-son 81’ the amphiploid between ‘Chinese Spring’ and A. junceum, A. junceum and amphiploid × wheat hybrids, Apart from germination in petridishes all other tests were carried out on plants grown in saline hydroculture tanks. Fresh weight measurements are given for stressed and non-stressed plants as well as measurements of harvest ripe plants. The utility of A. junceum as a source of salt tolerance genes for wheat is discussed.
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  • 4
    ISSN: 1432-2048
    Keywords: 15N natural abundance ; Genetic variation ; Hordeum ; Salt stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Given a uniform N source, the δ15N of barley shoots provided a genotypic range within treatments and a separation between control and salt-stress treatments as great as did δ13C*. Plant δ15N has been represented in the literature as a bioassay of external source δ15N and used to infer soil N sources, thus precluding consideration of the plant as a major cause in determining its own 815N. We believe this to be the first report of plant δ15N as a genetic trait. No mechanistic model is needed for use of δ15N as a trait in controlled studies; however, a qualitative model is suggested for further testing.
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  • 5
    ISSN: 1432-2242
    Keywords: Barley ; Ear emergence ; Height ; Linkage ; Quantitative inheritance ; Vernalization requirement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The quantitative traits height and ear-emergence date were analyzed in the F2 progeny of a cross between a tall winter barley cultivar (Gerbel) and a short spring barley cultivar (Heriot). The trait distributions were found to be related to the genotypes at two biochemical loci, β-amylase (Bmy1) and water-soluble protein (Wsp3), which are known to lie on the long arm of chromosome 4. Linkages between each trait and the markers were investigated using normal mixture models. The two parental phenotypes and the heterozygote phenotype of Bmy1 were distinguishable so the model could be used directly to estimate linkage between Bmy1 and a quantitative trait locus (QTL) for height (Height). The Gerbel homozygote and heterozygote phenotype of Wsp3 could not be distinguished and the model was adapted accordingly. The proportion of plants requiring vernalization was consistent with control by two independent genes acting epistatically, and a normal mixture model based on a two-gene hypothesis was fitted to the distribution of ear-emergence date to estimate linkage between the marker loci and a QTL for ear-emergence date (Vrn1). The parameters of each model were the recombination fraction between the marker locus and the QTL and the means and standard deviations associated with each QTL genotype; these were estimated by maximum likelihood. The fitted distributions correspond well to those observed and the order of the loci along the chromosome is inferred to be Height — Vrn1 — Bmy1 — Wsp3, with Wsp3 being the most distal.
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  • 6
    ISSN: 1432-2242
    Keywords: Water-soluble protein (WSP) ; Barley ; β Amylase ; Linkage ; Spring/winter habit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Water-soluble proteins (WSP-2 and WSP-3) and β-amylase (β-AMY-1) were extracted from mature endosperms of 44 spring and 39 winter barley genotypes. The protein and enzyme isoforms were separated in isoelectric focusing gels with a pH gradient of 4–6.5. The Wsp-3 and β-Amy-1 loci were located to chromosomes 4H using the wheat/barley chromosome addition lines. Segregation analysis of F2 and doubled haploid populations showed Wsp-2 and β-Amy-1 to be tightly linked, with a map distance of 11 cMorgans. Isoforms of WSP-2 possessed similar pIs to that of WSP-3 and overlapping bands were observed in the gels. These bands segregated independently in F2 and doubled haploid populations, implying two unlinked genes. All three loci were found to be polymorphic: two alleles were detected at the Wsp-2 locus, three at Wsp-3 and two at β-Amy-1. The frequency of alleles at all three loci was found to be different in winter and spring genotypes. Spring genotypes possessed a wider range of phenotypes than winter genotypes. Spring and winter genotypes could be distinguished on the basis of WSP-3 and β- AMY-1 phenotypes. The linkage between Wsp-3 and β-Amy-1 loci and genes controlling spring/winter habit on chromosome 4H is discussed. It is concluded that Wsp-3 and β-Amy-1 can be used as genetic markers for spring/winter habit in barley genetic research and breeding.
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  • 7
    ISSN: 1432-2242
    Keywords: Barley ; QTLs ; Linkage ; Yield ; Markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Quantitative trait loci (QTLs) have been revealed for characters in a segregating population from a spring barley cross between genotypes adapted to North-West Europe. Transgressive segregation was found for all the characters, which was confirmed by the regular detection of positive and negative QTLs from both parents. A QTL for all the agronomic, yield and grain characters measured except thousand grain weight was found in the region of the denso dwarfing gene locus. There were considerable differences between the location of QTLs found in the present study and those found in previous studies of North American germ plasm, revealing the diversity between the two gene pools. Thirty-one QTLs were detected in more than one environment for the 13 characters studied, although many more were detected in just one environment. Whilst biometrical analyses suggested the presence of epistasis in the genetic control of some characters, there was little evidence of interactions between the QTLs apart from those associated with yield. QTLs of large effect sometimes masked the presence of QTLs of smaller effect.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 80 (1990), S. 806-809 
    ISSN: 1432-2242
    Keywords: Milling energy (ME) ; Common wheat ; Aneuploid analysis ; Alien chromosome ; Triticeae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Aneuploid stocks, which included Triticum aestivum/alien, disomic, chromosome addition lines, wheat/alien, ditelosomic, chromosome addition lines, and the available aneuploids of “Chinese Spring” wheat, were used to locate genes that influence milling energy requirement (ME). Genes that affected ME were found on all seven homoeologous chromosome groups. The addition of complete wheat chromosomes 1B, 1D, 2A, 2D, 5B, 6B, 7B and 7D increased ME. Positive effects were also found in specific chromosome arms: 1BS, 2DS, 5AS, 5BS and 6BL. Wheat chromosome 3B conditioned low ME and the gene(s) responsible was located on the short arm. Other negative effects were attributed to wheat chromosome arms 4BL, 4DL, 5DS and 6DS. Alien chromosome additions that conferred high ME included 2H, 5H, 6H and 7H of barley, Hordeum vulgare and 2R, 2Rα, 4R, 4RL, 6R, 6RL and 7RL of rye, Secale cereale. Those that conferred a low ME included 1H ch of H. chilense, and 6u and 7u of Aegilops umbellulata, 5R and 5RS of S. cereale and 5R m and 5R mS of S. montanum. Although the control of ME is polygenic, there is a major effect of genes located on the short arms of homoeologous group 5 chromosomes.
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  • 9
    ISSN: 1432-2242
    Keywords: Doubled haploids ; Microspore ; Isozymes ; RFLPs ; Barley
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Biochemical, molecular and morphological markers have been used to monitor the segregation of alleles at major gene loci in microspore-derived lines of four spring barley crosses and their parents. Significant deviations from the expected Mendelian ratios were observed for four of the ten markers studied in the cross. Distorted ratios were associated with loci located on chromosomes 4H and 6H. The differential transmission of alleles was in favour of the responsive parent (Blenheim) used in the anther culture studies. For the α-Amy-1 locus on chromosome 6H, the preferential transmission of Blenheim alleles was most pronounced in the haploid regenerants that were colchicine treated. These results are discussed in relation to the genetic control of androgenetic response in barley and with respect to the exploitation of another culture in barley improvement.
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  • 10
    ISSN: 1432-2242
    Keywords: Wheat -Agropyron intermedium derivatives ; Stem rust ; Leaf rust ; Stripe rust ; C-banding ; In situ hybridization ; Isozyme analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Chromosome constitutions of three wheat-Agropyron intermedium derivatives were identified by C-banding analysis, in situ hybridization using biotin-labeled genomic Ag. intermedium DNA as a probe and isozyme analysis. Lines W44 and W52 were identified as 7Ai-2(7D) and 7Ai-2(7A) chromosome substitution lines carrying the same chromosome pair of Ag. intermedium. The alien chromosome was found to be homoeologous to group 7 based on C-banding, meiotic pairing and isozyme analyses. Line W49 was identified as a wheat Ag. intermedium chromosome translocation line. The breakpoint of the T2AS · 2AL-7Ai-2L translocation is located in the long arm at a fraction length of 0.62, and the transferred Ag. intermedium segment has a size of about 2.4 μm. Lines W44 and W52 expressed Ag. intermedium genes for resistance to leaf rust, stripe rust and stem rust, but only leaf rust resistance was expressed in W49. The results show that the leaf rust resistance gene(s), designated Lr38, is located in the distal half of the long arm of chromosome 7Ai-2, whereas the genes for resistance to stem rust and stripe rust are located either in the short arm or in the proximal region of the long arm of this chromosome.
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