Publication Date:
1990-03-16
Description:
Prothoracicotropic hormone (PTTH), a brain secretory polypeptide of insects, stimulates the prothoracic glands to produce and release ecdysone, the steroid essential to insect development. The complementary DNAs encoding PTTH of the silkmoth Bombyx mori were cloned and characterized, and the complete amino acid sequence was deduced. The data indicated that PTTH is first synthesized as a 224-amino acid polypeptide precursor containing three proteolytic cleavage signals. The carboxyl-terminal component (109 amino acids) that follows the last cleavage signal represents one PTTH subunit. Two PTTH subunits are linked together by disulfide bonds, before or after cleavage from prepro-PTTH, to form a homodimeric PTTH. When introduced into Escherichia coli cells, the complementary DNA directed the expression of an active substance that was functionally indistinguishable from natural PTTH. In situ hybridization showed the localization of the prepro-PTTH mRNA to two dorsolateral neurosecretory cells of the Bombyx brain.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kawakami, A -- Kataoka, H -- Oka, T -- Mizoguchi, A -- Kimura-Kawakami, M -- Adachi, T -- Iwami, M -- Nagasawa, H -- Suzuki, A -- Ishizaki, H -- New York, N.Y. -- Science. 1990 Mar 16;247(4948):1333-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biology, School of Science, Nagoya University, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2315701" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Base Sequence
;
Bombyx/*genetics/physiology
;
Cloning, Molecular
;
DNA/genetics
;
Gene Expression
;
Insect Hormones/*genetics
;
Molecular Sequence Data
;
Neurosecretory Systems/physiology
;
Nucleic Acid Hybridization
;
Protein Precursors/genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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