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  • Springer  (94)
  • 1990-1994  (50)
  • 1985-1989  (22)
  • 1975-1979  (21)
  • 1950-1954  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Carbohydrates of influenza virus. Structure of the oligosaccharides linked to asparagines 406 and 478 in the hemagglutinin of fowl plague virus, strain dutch (1987)
    Springer
    Glycoconjugate journal 4 (1987), S. 17-32 
    Details
    ISSN: 1573-4986
    Keywords: influenza virus ; hemagglutinin ; glycoprotein glycans ; oligosaccharide structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Fowl plague virus, strain Dutch, was metabolically labeled withd-[2-3H]mannose, or withd-[6-3H]glucosamine, and the small subunit (HA2; 0.8 mg in total) of the viral hemagglutinin was isolated by preparative sodium dodecylsulfate-polyacrylamide gel electrophoresis. After proteolytic digestion, the radioactive oligosaccharides were sequentially liberated from the glycopeptides by treatment with different endo-β-N-acetylglucosaminidases and with peptide:N-glycosidase or, finally, by hydrazinolysis. In this manner, four groups of glycans could be obtained by consecutive gel filtrations and were subfractionated by HPLC. The structures of the individual oligosaccharides were analyzed by micromethylation, by acetolysis or by digestion with exoglycosidases. The major species amongst the high mannose glycans at Ans-406 of the viral glycopolypeptide were found to be Manα1-2Manα1-3(Manα1-2Manα1-6)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNacβ1-4GlcNAc and Manα1-3(Manα1-2Manα1-6)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc, while the complex glycans at Asn-478 are predominantly GlcNAcβ1-2Manα1-3(GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAc (lacking, in part, one of the outerN-acetylglucosamine residues) and GlcNAcβ1-2Manα1-3(Galβ1-4GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAc.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01048441
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  • 2
    Electronic Resource
    Electronic Resource
    Glycosylation of glycoprotein 55 encoded by the anaemia-inducing strain of Friend spleen focus-forming virus (1994)
    Springer
    Glycoconjugate journal 11 (1994), S. 133-139 
    Details
    ISSN: 1573-4986
    Keywords: Friend spleen focus-forming virus ; glycoprotein ; oligosaccharide processing ; SFFV
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Normal rat kidney cells, non-productively infected with the anaemia-inducing variant of Friend spleen focus-forming virus (F-SFFVA), were metabolically labelled with [2-3H]mannose. The primary translation product of the viral envelope gene (env), representing a glycoprotein with an apparent molecularM r of 55 000 (gp55), was isolated from cell lysates by immunoaffinity chromatography and purified by preparative SDS/PAGE. Radiolabelled oligosaccharides, released from tryptic glycopeptides by treatment with endo-β-N-acetylglucosaminidase H, were characterized chromatographically, by enzymic digestion and by acetolysis. The results revealed that F-SFFVA gp55 obtained from this source carried predominantly oligomannose type sugar chains with five to nine mannoses. As a characteristic feature, glycans with seven to nine mannoses contained, in part, an additional glucose residue. Although the amount of glucosylated species found was higher in F-SFFVA gp55 (about 25% of total endo-H-sensitive oligosaccharides) than in gp55 of the corresponding polycythaemia-inducing variant (F-SFFVP, 16.3%), the overall glycosylation pattern of the F-SFFVA env product closely resembled that of F-SFFVP gp55 [Strubeet al. (1988)J Biol Chem 263:3762–71]. Hence, our results demonstrate that the different intracellular processing and transport of the primary F-SFFVA env product cannot be attributed to aberrant trimming of its oligomannose type glycans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00731153
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  • 3
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    Carbohydrates of influenza virus. Structure of the oligosaccharides linked to asparagines 406 and 478 in the hemagglutinin of fowl plague virus, strain dutch (1987)
    Springer
    Details
    Publication Date: 1987-01-01
    Print ISSN: 0282-0080
    Electronic ISSN: 1573-4986
    Topics: Chemistry and Pharmacology
    Published by Springer
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  • 4
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    Glycosylation of glycoprotein 55 encoded by the anaemia-inducing strain of Friend spleen focus-forming virus (1994)
    Springer
    Details
    Publication Date: 1994-04-01
    Print ISSN: 0282-0080
    Electronic ISSN: 1573-4986
    Topics: Chemistry and Pharmacology
    Published by Springer
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  • 5
    Electronic Resource
    Electronic Resource
    The effect of the environment on the permeability and composition of Citrus leaf cuticles (1990)
    Springer
    Planta 180 (1990), S. 147-153 
    Details
    ISSN: 1432-2048
    Keywords: Citrus ; Cuticle (composition, permeability) ; Humidity and cuticle permeability ; Temperature and cuticle permeability ; Wax (cuticle)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The water permeabilities of 3174 astomatous, isolated cuticular membranes from Citrus aurantium L. leaves were studied. Trees were grown in environmental chambers at temperatures ranging from 15 to 35° C and humidities of 50% and 90%. Photosynthetically active radiation was 500–1000 μmol photons·m−2· s−1. The different growing conditions had no effect on the water permeability of the membranes. However, storing isolated cuticular membranes at 8° C for up to 112 weeks decreased their water permeability, and it is argued that this is a consequence of the healing of defects between wax crystallites, and also indicates the dynamic nature of cuticular waxes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00193989
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  • 6
    Electronic Resource
    Electronic Resource
    Reelle algebraische Funktionen mit vorgegebenen Null- und Polstellen (1977)
    Springer
    Manuscripta mathematica 22 (1977), S. 87-103 
    Details
    ISSN: 1432-1785
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract This note continues the investigations of Knebusch on algebraic curves over real closed fields and was initiated by reading [3]. Especially we ask for the existence of real algebraic functions with given zeroes and poles, a question going back to Witt [4]. We study the real nature of coverings of real algebraic curves, and if the covering has degree two, we get algebraic proofs for results, which in the classical case have been obtained by topological methods in [2].
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01182069
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  • 7
    Electronic Resource
    Electronic Resource
    Electromagnetic fields of a vertical magnetic dipole above a laterally inhomogeneous thin layer of conductive overburden (1978)
    Springer
    Pure and applied geophysics 116 (1978), S. 181-197 
    Details
    ISSN: 1420-9136
    Keywords: Electromagnetic field ; Vertical magnetic dipole ; Thin conductive sheet ; Lateral conductivity variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract An analytical formulation is developed for the resultant electromagnetic field of an oscillating vertical magnetic dipole located over a thin conductive sheet of infinite extent. The sheet is characterized by a conductivity-thickness product or conductance σd that may be a function of the horizontal coordinates. The system of integral equations arising in the general formulation is simplified greatly when azimuthal symmetry prevails. Numerical results for a Gaussian variation of σd in the radial direction are presented for the case of a symmetrically located source. These results are for the fields at the level of the source dipole over the conductive sheet. It is shown that the quadrature response of the sheet is enhanced when there is rapid variation of the conductance. The null in the resultant wave tilt is also found to be shifted toward the direction of increasing conductance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00878992
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  • 8
    Electronic Resource
    Electronic Resource
    Anisotropy and temperature dependence of small angle neutron scattering from silicon-oxide precipitates in silicon (1991)
    Springer
    Applied physics 53 (1991), S. 422-425 
    Details
    ISSN: 1432-0630
    Keywords: 61.12Dw ; 72.10 ; 78.50G
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Czochralski grown silicon crystals contain interstitially dissolved oxygen which diffuses on heating to form precipitates of silica. We have examined these precipitates by small angle neutron scattering (SANS) in the Q-range 0.05 Å−1〈Q〈0.4 Å−1. The obtained SANS patterns reveal pronounced anisotropic intensity distributions which resemble the symmetry of the host crystal. The SANS spectra show an anisotropic central peak at Q〈0.1 Å−1 due to the single particle shape and a number of weak intensities for larger Q-values. These weak side maxima are considered correlation peaks or quasi-elastic interference peaks. They show, however, an unexpected and distinct temperature dependence: with decreasing temperature below values of 220 K their intensity is lost slowly, but reversibly. At T = 50 K only the central peak from the single-particle scattering remains unchanged. Upon heating, the correlation peaks regain their former value of intensity and Q-position without any evidence of thermal hysteresis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00348156
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  • 9
    Electronic Resource
    Electronic Resource
    Small angle neutron scattering from oxygen precipitates in silicon (1992)
    Springer
    Applied physics 54 (1992), S. 19-21 
    Details
    ISSN: 1432-0630
    Keywords: 61.12Dw ; 72.10 ; 78.50G
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The formation of silicon oxide precipitates from Czochralski grown silicon depends on the time and temperature of the heat treatment as well as on the initial content of interstitially dissolved oxygen. Samples containing between 5×1017 Oi/cm3 and 13×1017 Oi/cm3 have been heated at 750° C for 96 h. SiO2 precipitates of various shape and size have been obtained and investigated by means of small angle neutron scattering (SANS) in the Q-range 0.05 Å−1〈Q〈0.2 Å−1. The obtained SANS patterns reveal a typical anisotropy of their intensity distribution, which splits into a central peak at Q〈0.1 Å−1 due to the shape of the individual particles and a number of weak intensities for large Q-values, originating from a correlation between defects, possibly between the precipitates. While these correlation peaks in the SANS patterns are seen best for rather low values of about (5–7)×1017 Oi/cm3 oxygen content, the central peak anisotropy is most pronounced for higher values of ca 10×1017 Oi/cm3. The integrated intensity of the central peak increases with increasing initial oxygen content. For comparison, untreated samples of the same initial oxygen content do not reveal any anisotropic SAN scattering or a broadened central peak beam.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00348123
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  • 10
    Electronic Resource
    Electronic Resource
    Untersuchungen über die anisotrope Toluidinblaufärbung der Glykokalyx (1977)
    Springer
    Histochemistry and cell biology 50 (1977), S. 261-270 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung An der Erythrocytenmembran wird die Beziehung zwischen anisotroper Toluidinblaufärbung und Membranstruktur untersucht. Enzymatischer oder chemischer Abbau der Glykokalyx stört die topo-optische Färbung. Eine Abschwächung wird auch durch Digitoninbehandlung Glutaraldehyd-fixierter Zellen erzielt. Durch Chloroform-Methanol-Inkubation wird die anisotrope Toluidinblaufärbung gelöscht, aber sie kann nach Aldehyd-Bisulfitbildung wieder nachgewiesen werden. An agglutinierten Erythrocyten ist die Wirkung einer Chloroform-Methanol-Behandlung stark eingeschränkt. Die reversibel kollabierte Glykokalyx zeigt keine topo-optische Färbung. Die Befunde werden als Hinweis auf die Bedeutung der räumlichen Orientierung der Glykoproteine an der Zelloberfläche gedeutet. Störungen im Aufbau der Glykokalyx durch Hydrolyse bzw. Verlust oder durch Umverteilung von Komponenten verändern die Bedingungen für die anisotrope Toluidinblaufärbung. Eingriffe an der Lipidschicht können mit solchen Veränderungen einhergehen; für die Interkalation von Farbstoffmolekülen in die Lipidschicht ergab sich jedoch kein Anhalt. Die topo-optische Färbung mit Toluidinblau wird auf Grund dieser Ergebnisse als geeignetes Verfahren zur Untersuchung der Glykokalyx von Erythrocyten (und vermutlich auch anderer Zellen) angesehen.
    Notes: Summary The study was concerned with the molecular structure of the erythrocyte membrane and its anisotropic staining with toluidine blue. Enzymatic or chemical degradation impairs the topo-optical staining. Decreased anisotropy results also from digitonin treatment of glutaraldehyde fixed cells. Incubation in a mixture of chloroform and methanol abolishes the anisotropic staining with toluidine blue, but it has been restored by the aldehyde bisulfite-procedure. The efficacy of a treatment with chloroform-methanol is greatly reduced in the case of agglutinated erythrocytes. The reversibly collapsed glycocalyx is devoid of topo-optical staining. The findings refer to the spatial orientation of glycoproteins at the cell surface. Degradation or loss and rearrangement, respectively, change the glycocalyx structure and likewise deteriorate the conditions for the anisotropic staining. Alterations of the lipid layer of the membrane may result in similar effects. There was, however, no indication of an intercalation into the lipid layer of dyestuff molecules. In conclusion, the topo-optical toluidine blue staining is considered a powerful method aiding studies of the glycocalyx of erythrocytes (and presumably of other types of cells too).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00491073
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