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1

Electronic Resource

Photoelectric properties of structures based on TlInS2 single crystals (1998)

Iida, S. ; Mamedov, N. ; Rud’, V. Yu. ; [et al.]

Springer

Semiconductors 32 (1998), S. 67-70

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ISSN: 1063-7826

Source: Springer Online Journal Archives 1860-2000

Topics: Electrical Engineering, Measurement and Control Technology , Physics

Notes: Abstract The photovoltaic effect in heterocontacts of various types, viz., In/TlInS2, InSe/TlInS2, and GaSe/TlInS2, is investigated. The relative photoconversion quantum efficiency of these structures is studied as a function of the energy of the incident photons and the polarization plane of linearly polarized light. It follows from photosensitivity measurements that the photosensitive structures obtained can be employed as broad-band and selective photosensors of optical radiation.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1134/1.1187361

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2

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Somatic mutations caused by excision of the transposable element, Tpn1, from the DFR gene for pigmentation in sub-epidermal layer of periclinally chimeric flowers of Japanese morning glory and their germinal transmission to their progeny (1996)

Inagaki, Y. ; Hisatomi, Y. ; Iida, S.

Springer

Theoretical and applied genetics 92 (1996), S. 499-504

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ISSN: 1432-2242

Keywords: Transposable elements ; Periclinal chimera ; Somatic mutation ; Germinal transmission ; Japanese morning glory

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Pigmentation in flowers of Japanese morning glory is intense in the epidermal layer, lighter in the subepidermis, and much lighter in the internal tissues; by contrast coloration in stems occurs only in the sub-epidermal layer. The a-3 f mutant of Japanese morning glory bears white flowers with normal-colored flecks and sectors, and its variegation also occurs in leaves and stems. The mutable line can produce chimeric flowers pigmented uniformly in the sub-epidermal tissue and variegated in the epidermal layer, and stems of these flowers are also pigmented. Since they give selfed progeny that segregate to give a ratio of three germinal revertants bearing fully colored flowers to one flecked mutant, it has been [OR Imai (1934) has] postulated that somatic mutations in the sub-epidermal layer can be transmitted to the next generation and that the germ cells in the reproductive organs must form from the cells of the sub-epidermal layer. Recently, we found that the 6.4-kb En/Spm-related transposable element, Tpn1, resides within the DFR-B gene for anthocyanin biosynthesis in the mutable a-3 f line. To test whether somatic mutations caused by Tpn1 excision from the DFR-B gene in the subepidermis of periclinally chimeric flowers are transmissible to their progeny, we have examined the structure of the DFR-B region in the germinal revertants derived from the chimeric flowers and compared the sequences generated by the somatic excision of Tpn1 in periclinally chimeric flowers with those in their germinal revertants. Our results confirm that somatic mutations caused by Tpn1 excision from the DFR-B gene in the sub-epidermal tissue of chimeric flowers can be transmitted to their progeny, which results in the generation of germinal revertants.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00224550

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3

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DNA rearrangements at the region of the dihydroflavonol 4-reductase gene for flower pigmentation and incomplete dominance in morning glory carrying the mutable flaked mutation (1997)

Hisatomi, Y. ; Yoneda, Y. ; Kasahara, K. ; [et al.]

Springer

Theoretical and applied genetics 95 (1997), S. 509-515

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ISSN: 1432-2242

Keywords: Key words Allelic interaction ; Dihydroflavonol 4-reductase gene ; Mobile element-like sequences ; Morning glory ; Mutable allele

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The a-3 flecked [J] variegated line of Japanese morning glory bearing white flowers with normal-colored flecks and sectors has been shown to carry a 6.4-kb transposable element, Tpn1, inserted within the DFR-B gene, one of the anthocyanin biosynthesis genes encoding dihydroflavonol 4-reductase (DFR). The a flaked [M] variegated line of morning glory also bears white flowers with normal-colored flakes and sectors, and it was shown to carry multiple DNA rearrangements, including insertions of mobile element-like sequences, MELSIP1 and MELSIP2, in its DFR gene region. Unlike the a-3 flecked [J] mutation, the mutable a flaked [M] allele exhibited incomplete dominance. Interestingly, not only intensely colored flakes but also white spots and sectors were often observed in lightly colored flowers of morning glory in the heterozygous state A[M]/a flaked [M]. The interspecific F1 hybrids between Japanese morning glory and morning glory carrying both a-3 flecked [J]/A-3[M] and A[J]/ a flaked [M] in the heterozygous condition bear lightly colored flowers with intensely colored sectors as well as white flakes. The results clearly demonstrated that the DFR gene in the a flaked [M] line of morning glory is active and complements the DFR-B gene carrying Tpn1 in the a-3 flecked [J] line of Japanese morning glory. Interspecific allelic interactions between the mutable a flaked [M] gene of morning glory and the corresponding wild-type A[J] gene of Japanese morning glory resulted in incomplete dominance and the formation of white flakes and sectors. The appearance of the white flakes may be due to a somatic mutation of the A[J] gene.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s001220050591

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4

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The retrotransposon RTip1 is integrated into a novel type of minisatellite, MiniSip1, in the genome of the common morning glory and carries another new type of minisatellite, MiniSip2 (1997)

Hisatomi, Y. ; Hanada, K. ; Iida, S.

Springer

Theoretical and applied genetics 95 (1997), S. 1049-1056

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ISSN: 1432-2242

Keywords: Key words DNA rearrangement ; Minisatellite ; Morning glory ; Repetitive DNA ; Retrotransposon

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Transposable elements have often been discovered as new insertion sequences in known genes, and minisatellites are often employed as molecular markers in diagnostic and mapping studies. We compared the genes for flower pigmentation in a line of the common morning glory bearing fully colored flowers with those in two anthocyanin flaked mutable lines producing variegated flowers and found RFLPs at the region of the ANS gene for anthocyanin biosynthesis. The DNA rearrangements detected by the RFLPs are due to integration of a novel type of minisatellite, MiniSip1, having a long LTR retrotransposon, RTip1, inserted in the mutable lines. The structural analysis of the rearranged region revealed that the 12.4-kb RTip1 element is flanked by 5-bp target duplications within the MiniSip1 sequence and contains two LTR sequences of about 590 bp, a primer binding site for tRNALys, a typical polypurine tract and another new type of minisatellite, MiniSip2. Since no long open reading frame corresponding to the gag and pol genes was found, RTip1 appears to be a defective Ty3/gypsy-like element. Interestingly, the 269-bp-long MiniSip1 element comprises two alternating motifs of 41 bp and 19 bp, whereas the 962 bp long MiniSip2 element consists of two partially alternating motifs of 86 bp and 90 bp which are partially homologous to each other. Possible evolutionary processes that may have generated the rearranged structure at the ANS gene region are also discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s001220050661

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5

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Somatic mutations caused by excision of the transposable element, Tpn1, from the DFR gene for pigmentation in sub-epidermal layer of periclinally chimeric flowers of Japanese morning glory and their germinal transmission to their progeny (1996)

Inagaki, Y. ; Hisatomi, Y. ; Iida, S.

Springer

Theoretical and applied genetics 92 (1996), S. 499-504

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ISSN: 1432-2242

Keywords: Key words Transposable elements ; Periclinal chimera ; Somatic mutation ; Germinal transmission ; Japanese morning glory

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Pigmentation in flowers of Japanese morning glory is intense in the epidermal layer, lighter in the sub-epidermis, and much lighter in the internal tissues; by contrast coloration in stems occurs only in the sub-epidermal layer. The a-3 f〉 mutant of Japanese morning glory bears white flowers with normal-colored flecks and sectors, and its variegation also occurs in leaves and stems. The mutable line can produce chimeric flowers pigmented uniformly in the sub-epidermal tissue and variegated in the epidermal layer, and stems of these flowers are also pigmented. Since they give selfed progeny that segregate to give a ratio of three germinal revertants bearing fully colored flowers to one flecked mutant, it has been [OR Imai (1934) has] postulated that somatic mutations in the sub-epidermal layer can be transmitted to the next generation and that the germ cells in the reproductive organs must form from the cells of the sub-epidermal layer. Recently, we found that the 6.4-kb En/Spm-related transposable element, Tpn1, resides within the DFR-B gene for anthocyanin biosynthesis in the mutable a-3 f line. To test whether somatic mutations caused by Tpn1 excision from the DFR-B gene in the sub-epidermis of periclinally chimeric flowers are transmissible to their progeny, we have examined the structure of the DFR-B region in the germinal revertants derived from the chimeric flowers and compared the sequences generated by the somatic excision of Tpn1 in periclinally chimeric flowers with those in their germinal revertants. Our results confirm that somatic mutations caused by Tpn1 excision from the DFR-B gene in the sub-epidermal tissue of chimeric flowers can be transmitted to their progeny, which results in the generation of germinal revertants.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s001220050155

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6

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Mutants lacking glutelin subunits in rice: mapping and combination of mutated glutelin genes (1997)

Iida, S. ; Kusaba, M. ; Nishio, T.

Springer

Theoretical and applied genetics 94 (1997), S. 177-183

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ISSN: 1432-2242

Keywords: Key words Rice ; Mutation breeding ; Glutelin subunit ; RFLP mapping ; Low glutelin

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Nine mutant lines lacking glutelin subunits were selected from M2 seeds of about 10000 M1 plants mutagenized with gamma rays or EMS and from 1400 mutant lines selected originally for morphological characters. There were three types of mutants, one line lacking the largest subunit among four minor bands of glutelin acidic subunits (Type 1), five lines lacking the second largest subunit band (Type 2), and three lines lacking the third largest subunit band (Type 3). Mutants lacking the smallest subunit band were not found. Type 1 lacked 2 of the 10 spots of glutelin acidic subunits separated by two-dimensional electrophoresis and 1 of the 11 spots of the 57-kDa glutelin precursor. Type 2 lacked 2 spots of acidic subunits and 1 spot of the 57-kDa glutelin precursor, and had low amounts of 1 of the 8 spots of glutelin basic subunits. Type 3 mutants lacked each of 1 spot of the acidic subunits and glutelin precursor and had low amount of 1 spot of the basic subunits. Genetic analysis of the mutated genes showed that these mutant characters were controlled by single recessive genes named glu-1, glu-2, and glu-3, respectively. Mutated genes of different lines of the same type were found to be at the same locus. RFLP analysis of F2 plants between the mutant lines and cv `Kasalath' indicated that glu-1 is on chromosome 2, glu-2 on chromosome 10, and glu-3 on chromosome 1. These mutant genes were combined by crossing, and a line lacking the 3 minor bands of the glutelin acidic subunits was developed. However, the total glutelin content of this line was not remarkably reduced, showing a only 13% decrease.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s001220050397

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7

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Capture of a genomic HMG domain sequence by the En/Spm-related transposable element Tpn1 in the Japanese morning glory (1999)

Takahashi, S. ; Inagaki, Y. ; Satoh, H. ; [et al.]

Springer

Molecular genetics and genomics 261 (1999), S. 447-451

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ISSN: 1617-4623

Keywords: Key words Alternative splicing ; Gene capture ; HMG box ; Japanese morning glory ; Specialized transducing transposon

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The non-autonomous transposable element Tpn1 from the Japanese morning glory is an En/Spm-related DNA element found in the second intron of the DFR-B gene for flower pigmentation in the mutable line flecked, which shows variegation for flower color. It carries a genomic DNA segment containing at least four exon sequences encoding part of a HMG-box sequence. Spliced hybrid transcripts containing the DFR-B exon(s) and the HMG exons in Tpn1 were detected in the flower buds of the flecked line, and they were polyadenylated within Tpn1. Thus, Tpn1 can be regarded as a specialized transducing transposon carrying a part of the genomic sequence for a HMG box. The possible implications of the finding for evolution are discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s004380050987

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