ALBERT — All Library Books, journals and Electronic Records Telegrafenberg

1

Electronic Resource

Modified CTAB Procedure for DNA Isolation from Epiphytic Cacti of the Genera Hylocereus and Selenicereus (Cactaceae) (1999)

Tel-zur, N. ; Abbo, S. ; Myslabodski, D. ; [et al.]

Springer

Plant molecular biology reporter 17 (1999), S. 249-254

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ISSN: 1572-9818

Keywords: cacti ; DNA extraction ; Hylocereus ; polysaccharides ; Selenicereus

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract We present a simple protocol for DNA isolation from climbing cacti, genera Hylocereus and Selenicereus. The abundant polysaccharides present in Hylocereus and Selenicereus species interfere with DNA isolation, and DNA extracts, rich in polysaccharides, are poor templates for amplification using polymerase chain reaction (PCR). We used roots as the source tissue due to the lower viscosity of the extracts relative to that of other tissues. The extraction and isolation procedure we devised consists of the following steps: (1) three washes of ground tissue with the extraction buffer to remove the polysaccharides; (2) extraction with high-salt (4 M NaCl) cetyltrimethylammonium bromide (CTAB) buffer to remove the remaining polysaccharides; (3) removal of RNA by RNase; (4) phenol:chloroform extraction to remove proteins; (5) chloroform extraction to remove remaining phenols. The yields ranged from 10 to 20 μg DNA/g fresh roots. DNA samples prepared by our method were consistently amplifiable in the RAPD reaction and gave reproducible profiles.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1007656315275

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2

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Identification of a chromosome-specific probe that maps within the Ph1 deletions in common and durum wheat (1997)

Segal, G. ; Liu, B. ; Vega, J. M. ; [et al.]

Springer

Theoretical and applied genetics 94 (1997), S. 968-970

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ISSN: 1432-2242

Keywords: Key words Chromosome-specific DNA sequence ; Ph1 gene ; Wheat

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The Ph1 (pairing homoeologous) gene is the major factor that determines the diploid-like chromosome behavior of polyploid wheat. This gene, which is located on the long arm of chromosome 5B (5BL), suppresses homoeologous pairing at meiosis while allowing exclusive homologous pairing. In an effort to tag the specific chromosomal region where this gene is located, we have previously microdissected chromosome arm 5BL from bread wheat and produced a plasmid library by random PCR amplification and cloning. In this work we isolated from this library a 5BL-specific probe, WPG90, and mapped it within the interstitial deleted chromosome fragments carrying Ph1 in common and durum wheat. A PCR assay of Ph1 based on WPG90 was developed that allows an easy identification of homozygous genotypes deficient for this gene.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s001220050502

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3

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Characterization of the calmodulin gene family in wheat: structure, chromosomal location, and evolutionary aspects (1996)

Yang, Tianbao ; Segal, Gregorio ; Abbo, S. ; [et al.]

Springer

Molecular genetics and genomics 252 (1996), S. 684-694

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ISSN: 1617-4623

Keywords: Key words Aneuploid ; Calcium ; Root ; Triticum ; Wild wheat

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Calmodulin is a ubiquitous transducer of calcium signals in eukaryotes. In diploid plant species, several isoforms of calmodulin have been described. Here, we report on the isolation and characterization of calmodulin cDNAs corresponding to 10 genes from hexaploid (bread) wheat (Triticum aestivum). These genes encode three distinct calmodulin isoforms; one isoform is novel in that it lacks a conserved calcium binding site. Based on their nucleotide sequences, the 10 cDNAs were classified into four subfamilies. Using subfamily-specific DNA probes, calmodulin genes were identified and the chromosomal location of each subfamily was determined by Southern analysis of selected aneuploid lines. The data suggest that hexaploid wheat possesses at least 13 calmodulin-related genes. Subfamilies 1 and 2 were both localized to the short arms of homoeologous-group 3 chromosomes; subfamily 2 is located on all three homoeologous short arms (3AS, 3BS and 3DS), whereas subfamily 1 is located only on 3AS and 3BS but not on 3DS. Further analysis revealed that Aegilops tauschii, the presumed diploid donor of the D-genome of hexaploid wheat, lacks a subfamily-1 calmodulin gene homologue, whereas diploid species related to the progenitors of the A and B genomes do contain such genes. Subfamily 3 was localized to the short arm of homoeologous chromosomes 2A, 2B and 2D, and subfamily 4 was mapped to the proximal regions of 4AS, 4BL and 4DL. These findings suggest that the calmodulin genes within each subfamily in hexaploid wheat represent homoeoallelic loci. Furthermore, they also suggest that calmodulin genes diversified into subfamilies before speciation of Triticum and Aegilops diploid species.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02173974

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4

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Characterization of the calmodulin gene family in wheat: structure, chromosomal location, and evolutionary aspects (1996)

Yang, T. ; Segal, G. ; Abbo, S. ; [et al.]

Springer

Molecular genetics and genomics 252 (1996), S. 684-694

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ISSN: 1617-4623

Keywords: Aneuploid ; Calcium ; Root ; Triticum ; Wild wheat

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Calmodulin is a ubiquitous transducer of calcium signals in eukaryotes. In diploid plant species, several isoforms of calmodulin have been described. Here, we report on the isolation and characterization of calmodulin cDNAs corresponding to 10 genes from hexaploid (bread) wheat (Triticum aestivum). These genes encode three distinct calmodulin isoforms; one isoform is novel in that it lacks a conserved calcium binding site. Based on their nucleotide sequences, the 10 cDNAs were classified into four subfamilies. Using subfamily-specific DNA probes, calmodulin genes were identified and the chromosomal location of each subfamily was determined by Southern analysis of selected aneuploid lines. The data suggest that hexaploid wheat possesses at least 13 calmodulin-related genes. Subfamilies 1 and 2 were both localized to the short arms of homoeologous-group 3 chromosomes; subfamily 2 is located on all three homoeologous short arms (3AS, 3BS and 3DS), whereas subfamily 1 is located only on 3AS and 3BS but not on 3DS. Further analysis revealed thatAegilops tauschii, the presumed diploid donor of the D-genome of hexaploid wheat, lacks a subfamily-1 calmodulin gene homologue, whereas diploid species related to the progenitors of the A and B genomes do contain such genes. Subfamily 3 was localized to the short arm of homoeologous chromosomes 2A, 2B and 2D, and subfamily 4 was mapped to the proximal regions of 4AS, 4BL and 4DL. These findings suggest that the calmodulin genes within each subfamily in hexaploid wheat represent homoeoallelic loci. Furthermore, they also suggest that calmodulin genes diversified into subfamilies before speciation ofTriticum andAegilops diploid species.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02173974

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5

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Gibberellic acid (GA) increases fibre cell differentiation and secondary cell-wall deposition in spring wheat (Triticum aestivum L.) culms (1999)

Lev-Yadun, S. ; Beharav, A. ; Di-nur, R. ; [et al.]

Springer

Plant growth regulation 27 (1999), S. 161-165

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ISSN: 1573-5087

Keywords: cortical fibre cell ; gibberellic acid ; spring wheat ; Triticum aestivum

Source: Springer Online Journal Archives 1860-2000

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: Abstract The role of gibberellic acid (GA) in differentiation and secondary cell-wall deposition of fibre cells of spring wheat (Triticum aestivum) culms was studied using applications of GA and chlormequat (a GA biosynthesis inhibitor). In certain genotypes, higher GA levels may increase the number of cortical fibre cell files by changing cell fate from parenchyma to fibre, and induce thicker secondary cell-walls.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1006167531587

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6

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Organization of retro-element and stem-loop repeat families in the genomes and nuclei of cereals (1995)

Abbo, S. ; Dunford, R. P. ; Foote, T. N. ; [et al.]

Springer

Chromosome research 3 (1995), S. 5-15

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ISSN: 1573-6849

Keywords: Interphase ; physical organization ; repeated sequences

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Sequences homologous to the retro-element BIS-1 and the stem-loop repeat Hi-10 are present in the genomes of a number of cereal species. A detailed characterization of these elements indicated that they are non-randomly organized in the genomes of at least two of these species, namely barley and rye. In contrast to the BIS-1 retro-elements, the stem-loop repeats are also non-randomly organized into discrete domains in interphase nuclei from barley and rye. Features of the organization of these repeats along chromosomes and within interphase nuclei of rye, barley and rice are discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00711156

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7

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A Major Gene for Flowering Time in Chickpea (1999)

Or, E. ; Hovav, R. ; Abbo, S.

Springer

Crop science 39 (1999), S. 315-322

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ISSN: 1435-0653

Source: Springer Online Journal Archives 1860-2000

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: Cicer arietinum L.) cultivars begin flowering during the last week of March and their reproductive period extends throughout April to June. In the Middle East, April, May, and June are often dry and hot months. The objectives of this study were (i) to assess the potential range of chickpea germplasm as a source for early flowering, and (ii) to study the inheritance of the time to flowering trait. Germplasm evaluation was carried out by measuring days from germination to flowering and calculating phenotypic correlations between days to first flower, grain weight, and pod number along main branches. A number of early-flowering genotypes were identified, and weak association between flowering time genes and seed weight loci was observed. Crosses were made between types of contrasting photoperiod response. In F2 populations derived from crosses between an early-flowering breeding line (desi) with weak photoperiod response and a late-flowering high-yielding (kabuli) cultivar with a strong photoperiod response, a 3∝1 ratio of late-flowering: early-flowering types was observed. This segregation is consistent with action of a major photoperiod response gene (Ppd ) affecting time to flowering. Considerable genotype × environment interaction was observed among F3 progeny of these crosses. The relatively simple inheritance of the photoperiodic response observed in this study suggests that the early-flowering trait may be easily introduced into popular late-flowering genetic backgrounds.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/

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