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  • Articles  (23)
  • 1995-1999  (23)
  • Biology  (23)
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  • Articles  (23)
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  • 1
    ISSN: 1432-2056
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new “reef ” of the serpulid polychaete Serpula narconensis Baird, 1885 is reported from the South Georgia Island shelf. This particular mass occurrence is the second serpulid “reef” reported in the Antarctic region. Its location, at 91- to 105-m depth, is very different from those serpulid reefs previously reported, usually sited at intertidal zone or at low depth, and this may be one of the largest, if not the largest, serpulid reef in the world.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2056
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Spanish cruise “Bentart 94” was the first of two devoted to the study of the benthic marine fauna off Livingston and Deception Islands, South Shetland Islands, Antarctica. In this paper, the taxonomic results of the examination of the “errant” families of the polychaetes collected during that cruise are presented. A total of 834 specimens have been examined, belonging to 34 species of 31 genera and 11 families. A new species, Micronereis anaramosae, is described, and Eusyllis kerguelensis McIntosh is referred to the genus Pionosyllis. The specimens were found in 109 samples of muddy sand, mud, stones, rocks, gravel, and mixed sediments, by scuba diving, Van Veen grab, rock trawl and anchor dredge.
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  • 3
    ISSN: 1573-4919
    Keywords: Stypopodium flabelliforme ; 14-keto-stypodiol diacetate ; DU-145 cell line ; microtubule assembly ; cell proliferation ; plasminogen activator (u-PA) ; tumor invasiveness
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract We investigated the effects of the drug 14-keto-stypodiol diacetate (SDA) extracted from the seaweed product Stypopodium flabelliforme, in inhibiting the cell growth and tumor invasive behavior of DU-145 human prostate cells. In addition, the molecular action of the drug on microtubule assembly was analyzed. The effects of this diterpenoid drug in cell proliferation of DU-145 tumor cells in culture revealed that SDA at concentrations of 5 μM decreased cell growth by 14%, while at 45 μM a 61% decrease was found, as compared with control cells incubated with the solvent but in the absence of the drug. To study their effects on the cell cycle, DU-145 cells were incubated with increasing concentrations of SDA and the distribution of cell-cycle stages was analyzed by flow cytometry. Interestingly, the data showed that 14-keto-stypodiol diacetate dramatically increased the proportion of cells in the G2/M phases, and decreased the number of cells at the S phase of mitosis, as compared with appropriate controls. Studies on their action on the in vitro assembly of microtubules using purified brain tubulin, showed that SDA delayed the lag period associated to nucleation events during assembly, and decreased significantly the extent of polymerization. The studies suggest that this novel derivative from a marine natural product induces mitotic arrest of tumor cells, an effect that could be associated to alterations in the normal microtubule assembly process. On the other hand, a salient feature of this compound is that it affected protease secretion and the in vitro invasive capacity, both properties of cells from metastases. The secretion of plasminogen activator (u-PA) and the capacity of DU-145 cells to migrate through a Matrigel-coated membrane were significantly inhibited in the presence of micromolar concentrations of SDA. These results provide new keys to analyze the functional relationships between protease secretion, invasive behavior of tumor cells and the microtubule network.
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  • 4
    ISSN: 1432-2056
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Macrobenthic assemblages were investigated at 26 stations located around Livingston Island, Deception Island and the Bransfield Strait at depths ranging from 42 to 671 m. Representatives of 30 major taxa were found. The maximal density was 5,260 specimens·m−2 at Livingston Island; the mean abundance per station ranged from 160 to 4,380 specimens·m−2. The total biomass of the macrozoobenthos declined with depth, with mean values of 3,201 g·m−2 at shallower depths (〈 100 m) and 210 g·m−2 further down (〉 100 m). After multivariate analysis (cluster analysis, MDS) based on Bray-Curtis dissimilarities, most stations could be assigned to one of three groups on the basis of distinct biomass differences between sites. The first cluster with a rich Ascidiacea biomass is common on shallower bottoms. The second, with Ophiuroidea as a characteristic group, is common on deeper bottoms. The absence of an ‘indicator’ taxon is characteristic of the remaining cluster of those stations with the lowest biomass values. No significant correlations were detected between macrobenthic biomass and any sediment parameters measured, probably because part of the benthos (i.e. the epifauna) could be better explained by the coupling with a highly productive water column. The role of the epi-infauna sensu Gallardo as the main factor structuring benthic assemblages in the investigated area is discussed.
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  • 5
    ISSN: 1432-0878
    Keywords: Key words Thyroid gland ; Calcitonin ; C-cell hyperplasia ; C-cell carcinoma ; Medullary thyroid carcinoma ; Sexual dimorphism ; Rat (Wistar)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  In many rat strains, C-cell hyperplasia occurs in an age-dependent manner and is often associated with multifocal C-cell carcinoma. The purpose of this study was to investigate the spectrum of spontaneous, proliferative C-cell disorders by gender in Wistar rats throughout their lifespan. The incidence of C-cell hyperplasia shows a significant increase with age (P〈0.001) and is much higher in female rats than in male rats (P〈0.05). From 3 to 24 months of life, 27.5% of female rats showed a normal C-cell pattern, 55.0% showed C-cell hyperplasia, and 17.5% showed C-cell tumors; while 57.5% of male rats showed a normal C-cell pattern, 32.5% showed C-cell hyperplasia, and 10% showed C-cell tumors. Although the overall frequency of C-cell neoplasms in females was nearly double that in males, these data are not statistically significant. However, the number of C-cell tumors showed a significant increase with age (P〈0.05). Therefore, we can conclude that there were significant differences in the incidence of the total spectrum of C-cell proliferative abnormalities in the thyroid gland of Wistar rats that were both age-dependent and gender-dependent.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Plant ecology 130 (1997), S. 101-109 
    ISSN: 1573-5052
    Keywords: Distribution ; Ecology ; Morphology ; Nothofagus ; South American
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The morphology of the 11 taxa of South American Nothofagus are compared. Thirty eight characteristics were taken into account: 12 from leaves, 3 from stipules, 3 from buds, 6 from cupules, 7 from fruits, 4 from petioles and 3 from male flowers. The data matrix, with average values of 100 measurements per taxon, was analyzed with multivariate statistical methods of classification and polar and spatial ordination. Five groups were established: The first one (Nothofagus obliqua, N. obliqua var. macrocarpa, N. leoni and N. alessandrii), with deciduous leaves, is adapted to mediterranean climatic conditions. The second groups (N. dombeyi, N. nitida and N. antarctica), with small leaves which are evergreen for the first two species and deciduous for the last, develops under temperate humid conditions typical of the Valdivian region. The three remaining groups correspond to isolated species with different requirements: N. glauca, has large deciduous leaves and colonizes the most xerophytic biotope that a Nothofagus in central Chile can tolerate. N. pumilio, with mid-sized deciduous leaves, is adapted to the cold and dry zones of the southern Andes. Finally, N. betuloides, with small evergreen leaves, grows in the cold/humid somewhat boggy conditions of the Magellanic region.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    International journal of anthropology 14 (1999), S. 203-209 
    ISSN: 1824-3096
    Keywords: Menarcheal age ; Peak Height Velocity ; Preece-Baines Model I ; Basque Country
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Data on menarcheal age were collected by thestatus quo method on a sample of 808 schoolgirls (8–19 years of age), from the Margen Izquierda area of the Biscay province (Basque Country). Probit analysis provided a mean age at menarche of 12.5±0.05 years with a standard deviation of 1.0 years. The result was compared with data from other previous Basque studies and related to age at PHV of the same sample.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 13 (1997), S. 225-232 
    ISSN: 1573-0972
    Keywords: Biological control ; culture medium ; spore shelf-life ; storage conditions ; Trichoderma harzianum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The influence of pH, carbon:nitrogen (C:N) ratio, carbon content and harvesting time on spore attributes of the biocontrol agent Trichoderma harzianum was evaluated. The effect of these culture parameters on viability, shelf-life and ultrastucture was also assessed. pH was a key parameter to manipulate for both growth and sporulation, while carbon concentration and C:N ratio strongly affected spore production time. At fixed pH, the C:N ratio had a limited influence on production yield, but was critical for spore shelf-life. The highest spore longevity was found in a medium with a C:N ratio of 14 and a pH of 7.0, when most resulting spores were still alive after 45 d storage. These spores also remained viable during storage under a broad range of relative humidities, indicating that they would be more sustainable in the field.
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  • 9
  • 10
    Publication Date: 1998-10-01
    Print ISSN: 0031-9422
    Electronic ISSN: 1873-3700
    Topics: Biology , Chemistry and Pharmacology
    Published by Elsevier
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