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  • 1
    Electronic Resource
    Electronic Resource
    A multi-nucleate male-sterile mutant of maize with gametophytic expression (1997)
    Springer
    Sexual plant reproduction 10 (1997), S. 22-26 
    Details
    ISSN: 1432-2145
    Keywords: Key words Maize ; Pollen ; Male sterility ; Gametophytic mutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  In order to dissect the complex genetic system that controls pollen development, we have undertaken a program of transposon insertion mutagenesis, with the purpose of producing mutations in gametophytically acting genes that are important for this process. The present work reports the developmental cytology of one of the mutants isolated, gaMS-2 (gametophytic male sterile-2). A peculiar feature of the mutant grains was lack of differentiation between the vegetative and the generative nuclei, leading to alteration in number, conformation and placement of nuclei. At anthesis, the grains carrying the mutant allele are about 40% of the normal grain size, contain a very reduced amount of starch and exhibit various nuclear abnormalities.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050063
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular analysis and mapping of two genes encoding maize glutathione S-transferases (GST I and GST II) (1995)
    Springer
    Molecular genetics and genomics 248 (1995), S. 535-539 
    Details
    ISSN: 1617-4623
    Keywords: Glutathione S-transferase ; Maize ; Gene expression ; Mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Maize glutathione S-transferase (GST) isozymes are encoded by a gene family comprising at least five genes, three of which (Gst I, II andIII) have recently been isolated and sequenced. The enzymes are active as homo or heterodimers and exhibit intraspecific polymorphism including a “null” variant for the two major isoforms expressed in roots. Northern blot analyses performed on total root RNA from “null” and “plus” genotypes, usingGst I- andGst II-specific probes, indicated that theGst I gene controls the expression of the two major GST isoforms expressed in roots.Gst I andGst II were mapped by RFLP analysis using an F2 population of 149 individuals previously characterized.Gst I was localized on the long arm of chromosome 8, while two putativeGst II loci were mapped to chromosomes 8 (70 cM fromGst I) and 10, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02423448
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  • 3
    Electronic Resource
    Electronic Resource
    Identification of genetic factors for alachlor tolerance in maize by molecular markers analysis (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 551-555 
    Details
    ISSN: 1617-4623
    Keywords: Maize ; Herbicide ; Molecular markers ; Gametophytic selection ; Linkage analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genetic factors controlling tolerance to the herbicide Alachlor in maize were localised by means of two different strategies. In the first approach, backcross (BC) plants, derived from pollen which had been subjected to selective pressure for resistance to the herbicide, were analysed for segregation distortion at 47 RFLP loci and compared to BC plants obtained from non-selected pollen. Preferential transmission of five chromosomal regions where putative QTLs (Quantitative Trait Loci) are localised was revealed in the BC plants from selected pollen. A second approach was based on a classical linkage analysis for segregation of the same set of RFLPs and factors controlling the trait, in a BC population of 210 individuals, by means of regression analysis. This study detected seven significant loci in four genomic regions. Overall, two loci revealed both segregation distortion and association with the expression of the trait, indicating linkage to genes expressed in both gametophytic and sporophytic phase. Three chromosomal regions appeared to carry factors involved in plant tolerance to Alachlor which are not expressed in pollen. Conversely, three loci were linked to factors selectable in pollen, but did not reveal significant association with tolerance in the plant in the segregating populations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02173644
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  • 4
    Electronic Resource
    Electronic Resource
    Regiospecific Synthesis of 3,4-Disubstituted Furans and ThiophenesDedicated to Professor Xi-Kui Jiang, Shanghai Institute of Organic Chemistry, on the occasion of his 70th birthday. (1997)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1997 (1997), S. 459-466 
    Details
    ISSN: 0947-3440
    Keywords: Furans ; Thiophens ; Palladium catalysis ; Coupling reactions ; Heterocycles ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The regiospecific synthesis of 3,4-disubstituted furans and thiophenes is reviewed. Our initial approach used 3,4-bis(tri-n-butylstannyl)furan as a building block, which led to many structurally diverse 3,4-disubstituted furans. Another avenue was explored by using 3,4-bis(trimethylsilyl)furan and 3,4-bis(trimethylsilyl)thiophene as precursors. A variety of unsymmetrically 3,4-disubstituted furans and thiophenes were prepared in this way. The advantages of our strategy lie in its stepwise manner as well as its prospect of yielding 3,4-disubstituted furans and thiophenes with rather different substitution pattern.
    Additional Material: 5 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jlac.199719970304
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