Abstract
Genetic factors controlling tolerance to the herbicide Alachlor in maize were localised by means of two different strategies. In the first approach, backcross (BC) plants, derived from pollen which had been subjected to selective pressure for resistance to the herbicide, were analysed for segregation distortion at 47 RFLP loci and compared to BC plants obtained from non-selected pollen. Preferential transmission of five chromosomal regions where putative QTLs (Quantitative Trait Loci) are localised was revealed in the BC plants from selected pollen. A second approach was based on a classical linkage analysis for segregation of the same set of RFLPs and factors controlling the trait, in a BC population of 210 individuals, by means of regression analysis. This study detected seven significant loci in four genomic regions. Overall, two loci revealed both segregation distortion and association with the expression of the trait, indicating linkage to genes expressed in both gametophytic and sporophytic phase. Three chromosomal regions appeared to carry factors involved in plant tolerance to Alachlor which are not expressed in pollen. Conversely, three loci were linked to factors selectable in pollen, but did not reveal significant association with tolerance in the plant in the segregating populations.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Gressel J (1995) Catch 22 — Mutually exclusive strategies for delaying/preventing polygenically vs. monogenically inherited resistance. In: Ragsdale NN, Kearney PC, Plimmer JR (eds) 8th International Congress of Pesticide Chemistry. Options 2000. American Chemical Society, Washington DC, pp 330–339
Hormaza JI, Herrero M (1992) Pollen selection Theor Appl Genet 83:663–672
Irzyk GP, Fuerst EP (1993) Purification and characterisation of glutathione S-transferase from benoxacor-treated maize (Zea mays). Plant Physiol 102:803–810
Mozer TJ, Tiemeier C, Jaworski EG (1983) Purification and characterisation of corn glutathione S-transferase. Biochemistry 22:1068–1072
Ottaviano E, Sari-Gorla M (1993) Gametophytic and sporophytic selection. In: Hayward MD, Bosemark NO, Romagosa I (eds) Plant breeding — principles and prospects. Chapman and Hall, London, pp 332–352
Pè ME, Gianfranceschi L, Taramino G, Tarchini R, Angelini P, Dani M, Binelli G (1993) Mapping quantitative trait loci (QTLs) for resistance toGibberella zeae infection in maize. Mol Gen Genet 241:11–16
Pfahler PL (1983) Comparative effectiveness of pollen genotype selection in higher plants In: Mulcahy DL, Ottaviano E (eds) Biotechnology and ecology of pollen. Elsevier Biomedical, New York, pp 361–367
Rossini L, Pè ME, Frova C, Hein K, Sari Gorla M (1995) Molecular analysis and mapping of two genes encoding maize glutathione-S-transferases (GST I and GST II). Mol Gen Genet 248:535–539
Sari-Gorla M (1992) Effects of gametophytic selection on the genetic structure of populations. In: Cresti M, Tiezzi A (eds) Sexual plant reproduction. Springer-Verlag, Berlin, pp 151–159
Sari-Gorla M (1994) Pollen selection for tolerance to environmental stresses. In: Stephenson A, Kao T (Eds) Pollen pistil interaction and pollen tube growth. Curr Top in Plant Physiol 12:230–239
Sari-Gorla M, Ferrario S, Rossini L, Frova C, Villa M (1993) Developmental expression of glutathione S-transferase in maize and its possible connection with herbicide tolerance. Euphytica 86:213–220
Sari-Gorla M, Ferrario S, Frascaroli E, Frova C, Landi P, Villa M (1994) Sporophytic response to pollen selection for Alachlor tolerance in maize. Theor Appl Genet 88: 812–817
SAS Institute (1985) SAS User's Guide: Statistics. SAS Institute, Cary, N.C.
Shultz A, Wengermayer F, Goodman HM (1990) Genetic engineering of herbicide resistance in higher plants. Crit Rev Plant Sci 9:1–15
Stam P (1993) Construction of integrated genetic linkage maps by means of a new computer package: JoinMap. Plant J 3:739–744
Timmerman KP (1989) Molecular characterisation of corn glutathione S-transferase isozymes involved in herbicide detoxification. Physiol Plantarum 77:465–471
Wrubel RP, Gressel J (1994) Are herbicide mixtures useful for delaying the rapid evolution of resistance? A case study. Weed Technol 8:635–648
Author information
Authors and Affiliations
Additional information
Communicated by R. Hagemann
Rights and permissions
About this article
Cite this article
Sari-Gorla, M., Rampoldi, L., Binelli, G. et al. Identification of genetic factors for alachlor tolerance in maize by molecular markers analysis. Molec. Gen. Genet. 251, 551–555 (1996). https://doi.org/10.1007/BF02173644
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02173644