ISSN:
1573-4943
Keywords:
thiodan
;
sulfhydryl reagents
;
fluorescent probes for SH groups
;
fluorescence titration
;
chemical modification
;
dansyl chloride
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract A new fluorescent reagent, Thiodan, containing the -SO2-S- function, is described. It can be utilized in protein chemistry as a reversible fluorescent labeling agent of cysteine residues. Thiodan is also shown to be useful for titration of sulfhydryl groups, due to the intensely fluorescent sulfinate anion released during the reaction.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01025373
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