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  • 1
    Electronic Resource
    Electronic Resource
    Cultivation of hybridoma cells in an inclined bioreactor (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 45 (1995), S. 176-186 
    Details
    ISSN: 0006-3592
    Keywords: animal cell ; hybridoma cell ; shear ; cell damage ; bioreactor design ; inclination ; bubble column ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Murine hybridoma cells were grown in a bubble column that was inclined up to 45° from vertical. Inclining the column by a few degrees separated the rising bubbles against the upper surface, leaving the bulk of the liquid bubble free. The liquid was circulated well by the rising bubbles, but collection of cells by rising bubbles and exposure of cells to bursting bubbles were minimized. Maximum viable cell count and exponential growth of the cells were not affected by inclination, but an inclination of 30° gave an antibody titer of 42 mg/L, which more than doubled the yield of 17 mg/L in the vertical position. By comparison, the culture gave yields of 30 mg/L when grown in spinner flasks. The enhanced antibody production in the inclined bioreactor corresponded to a prolonged stationary phase of 45 h. © 1995 John Wiley & Sons, Inc.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260450212
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  • 2
    Electronic Resource
    Electronic Resource
    Solid phase synthesis of cyclic peptides: model studies involving i-(i+4) side chain-to-side chain cyclisation (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Peptide Science 4 (1998), S. 335-343 
    Details
    ISSN: 1075-2617
    Keywords: cyclic peptides ; human growth hormone ; lactam cyclisation ; side chain effects ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Conditions for the synthesis of i-(i+4) side chain-to-side chain head-to-tail Lys→Glu and Glu→Lys linked cyclic peptides related to hypoglycaemic analogues of human growth hormone hGH [6-13] have been examined. The success of the cyclisation reaction with the corresponding resin-bound, partially protected linear peptides was found to be both reagent as well as sequence dependent, with competing inter-chain oligomerisation predominating in some cases. The results also indicated that protection with the bulky Fmoc group of the amino acid residues immediately adjacent to the side chain-deprotected Lys and Glu residues, which participate in the cyclisation reaction, enhanced the rate of lactam formation. © 1998 European Peptide Society and John Wiley & Sons, Ltd.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    In vitro degradation of natural insoluble lignin in aqueous media by the extracellular peroxidases of Phanerochaete chrysosporium (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 57 (1998), S. 704-717 
    Details
    ISSN: 0006-3592
    Keywords: lignin peroxidase ; manganese peroxidase ; LIP ; MNP ; Phanerochaete chrysosporium ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The lignin peroxidases (LIP) and manganese peroxidases (MNP) of Phanerochaete chrysosporium catalyze a wide range of lignin depolymerization reactions with lignin models and synthetic lignins in solution. However, their ability to degrade insoluble natural lignin in aqueous media has not been demonstrated. Insoluble isolated poplar lignin similar to natural lignin was treated in vitro in aqueous media for 12 h with LIP, MNP, and both. Treatment with MNP alone slightly increased the solid mass and produced measurable amounts of lignin-derived 2,6-dimethoxyhydroquinone and 2-methoxyhydroquinone but did not appreciably decrease the total lignin content. Treatment with LIP alone did not decrease the mass but produced measurable amounts of lignin-derived p-hydroxybenzoic acid and slightly decreased the lignin content. Finally, treatment with LIP and MNP together decreased the solid mass by 11%, decreased the lignin content by 5%, and released low-concentration compounds with mass spectra containing the typical lignin-derived electron-impact fragments of mass 107, 137, 151, 167, and 181. These results suggest that MNP increases the effectiveness of LIP-mediated lignin degradation. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 57: 704-717, 1998
    Additional Material: 3 Ill.
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  • 4
    Electronic Resource
    Electronic Resource
    Shift from growth to nutrient-limited maintenance kinetics during biofilter acclimation (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 56 (1997), S. 330-339 
    Details
    ISSN: 0006-3592
    Keywords: biofilter ; kinetics ; maintenance metabolism ; acclimation ; biomass ; nutrient limitation ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: During long-term operation of a biofilter, the mandatory absence of net cell growth forces the cells into maintenance metabolism, which is of relatively low rate compared to substrate consumption during the active growth of the acclimation phase. A model based on this shift in metabolism can explain the postacclimation decrease in activity sometimes reported for biofilters. The cessation of growth can be caused by nutrient depletion in the bed. Postacclimation nutrient addition increases activity primarily by allowing a return to the high substrate consumption rate of active growth, and only secondarily helps raise bed activity because of the ultimately higher amount of biomass in the bed. Simulations incorporating the acclimation period and the role of maintenance metabolism predict about 4 logarithms of growth during acclimation of a hexane biofilter, which was confirmed experimentally. Changes in a biofilter's biomass during the acclimation phase can be estimated from substrate conversion data using two approximate methods. The first follows the cumulative amount of substrate converted and uses the estimated yield of cells from substrate during active growth to estimate the total biomass created. The second method follows a rate constant for conversion of substrate in the bed. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 56: 330-339, 1997.
    Additional Material: 6 Ill.
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  • 5
    Electronic Resource
    Electronic Resource
    Identification of procaryotic developmental stages by statistical analyses of two-dimensional gel patterns (1997)
    Weinheim : Wiley-Blackwell
    Electrophoresis 18 (1997), S. 1418-1428 
    Details
    ISSN: 0173-0835
    Keywords: Two-dimentional polyacrylamide gel electrophoresis ; Streptomyces ; Development ; Multivariate ; Regulation ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Multivariate statistical comparisons of two-dimensional protein (2-D) gel patterns were used for the first time to define stages of a biological developmental system. The differentiating procaryote, Streptomyces coelicolor, was radiolabeled in liquid cultures at 16 intervals during development, and radioactive proteins were separated and quantified on 2-D gels. Cluster, principal component, and correlation analyses classified these gel patterns into four distinct groups, each reflecting a pattern of gene expression specific for a stage of development. These studies focused our attention on a phase of arrested growth as a key regulatory transition leading to secondary metabolism and a phase of renewed growth. Proteins whose synthesis was switched on or off during the “transitional” phase (some 21 and 18, respectively) were identified and will be the focus of future studies designed to identify their physiological or regulatory function.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150180817
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  • 6
    Electronic Resource
    Electronic Resource
    A SPACE-TIME FINITE ELEMENT METHOD FOR THE EXTERIOR STRUCTURAL ACOUSTICS PROBLEM: TIME-DEPENDENT RADIATION BOUNDARY CONDITIONS IN TWO SPACE DIMENSIONS (1996)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 39 (1996), S. 1635-1657 
    Details
    ISSN: 0029-5981
    Keywords: finite element method ; radiation boundary conditions ; absorbing boundary conditions ; discontinuous Galerkin method ; structural acoustics ; wave equation ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A time-discontinuous Galerkin space-time finite element method is formulated for the exterior structural acoustics problem in two space dimensions. The problem is posed over a bounded computational domain with local time-dependent radiation (absorbing) boundary conditions applied to the fluid truncation boundary. Absorbing boundary conditions are incorporated as ‘natural’ boundary conditions in the space-time variational equation, i.e. they are enforced weakly in both space and time. Following Bayliss and Turkel, time-dependent radiation boundary conditions for the two-dimensional wave equation are developed from an asymptotic approximation to the exact solution in the frequency domain expressed in negative powers of a non-dimensional wavenumber. In this paper, we undertake a brief development of the time-dependent radiation boundary conditions, establishing their relationship to the exact impedance (Dirichlet-to-Neumann map) for the acoustic fluid, and characterize their accuracy when implemented in our space-time finite element formulation for transient structural acoustics. Stability estimates are reported together with an analysis of the positive form of the matrix problem emanating from the space-time variational equations for the coupled fluid-structure system. Several numerical simulations of transient radiation and scattering in two space dimensions are presented to demonstrate the effectiveness of the space-time method.
    Additional Material: 13 Ill.
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  • 7
    Electronic Resource
    Electronic Resource
    A Galerkin least-squares finite element method for the two-dimensional Helmholtz equation (1995)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 38 (1995), S. 371-397 
    Details
    ISSN: 0029-5981
    Keywords: Helmholtz equation ; least squares ; Galerkin method ; Fourier analysis ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: In this paper a Galerkin least-squares (GLS) finite element method, in which residuals in least-squares form are added to the standard Galerkin variational equation, is developed to solve the Helmholtz equation in two dimensions. An important feature of GLS methods is the introduction of a local mesh parameter that may be designed to provide accurate solutions with relatively coarse meshes. Previous work has accomplished this for the one-dimensional Helmholtz equation using dispersion analysis. In this paper, the selection of the GLS mesh parameter for two dimensions is considered, and leads to elements that exhibit improved phase accuracy. For any given direction of wave propagation, an optimal GLS mesh parameter is determined using two-dimensional Fourier analysis. In general problems, the direction of wave propagation will not be known a priori. In this case, an optimal GLS parameter is found which reduces phase error for all possible wave vector orientations over elements. The optimal GLS parameters are derived for both consistent and lumped mass approximations. Several numerical examples are given and the results compared with those obtained from the Galerkin method. The extension of GLS to higher-order quadratic interpolations is also presented.
    Additional Material: 20 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620380303
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  • 8
    Electronic Resource
    Electronic Resource
    Application of the full approximation storage method to the numerical simulation of two-dimensional steady incompressible viscous multiphase flows (1998)
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 28 (1998), S. 1217-1239 
    Details
    ISSN: 0271-2091
    Keywords: multiphase ; multifluid ; multigrid ; FAS ; local coupled solver ; Engineering ; Numerical Methods and Modeling
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: In recent years multigrid algorithms have been applied to increasingly difficult systems of partial differential equations and major improvements in both speed of convergence and robustness have been achieved. Problems involving several interacting fluids are of great interest in many industrial applications, especially in the process and petro-chemical sectors. However, the multifluid version of the Navier-Stokes equations is extremely complex and represents a challenge to advanced numerical algorithms. In this paper, we describe an extension of the full approximation storage (FAS) multigrid algorithm to the multifluid equations. A number of special issues had to be addressed. The first was the development of a customised, non-linear, coupled relaxation scheme for the smoothing step. Automatic differentiation was used to facilitate the coding of a robust, globally convergent quasi-Newton method. It was also necessary to use special inter-grid transfer operators to maintain the realisability of the solution. Algorithmic details are given and solutions for a series of test problems are compared with those from a widely validated, commercial code. The new approach has proved to be robust; it achieves convergence without resorting to specialised initialisation methods. Moreover, even though the rate of convergence is complex, the method has achieved very good reduction factors: typically five orders of magnitude in 50 cycles. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 13 Ill.
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  • 9
    Electronic Resource
    Electronic Resource
    NUMERICAL UNCERTAINTIES IN TRANSONIC FLOW CALCULATIONS FOR AEROFOILS WITH BLUNT TRAILING EDGES (1997)
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 24 (1997), S. 355-373 
    Details
    ISSN: 0271-2091
    Keywords: computational fluid dynamics ; transonic airfoils ; numerical uncertainty ; Engineering ; Numerical Methods and Modeling
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Numerical uncertainties are quantified for calculations of transonic flow around a divergent trailing edge (DTE) supercritical aerofoil. The Reynolds-averaged Navier-Stokes equations are solved using a linearized block implicit solution procedure and mixing-length turbulence model. This procedure has reproduced measurements around supercritical aerofoils with blunt trailing edges that have shock, boundary layer and separated regions. The present effort quantifies numerical uncertainty in these calculations using grid convergence indices which are calculated from aerodynamic coefficients, shock location, dimensions of the recirculating region in the wake of the blunt trailing edge and distributions of surface pressure coefficients. The grid convergence index is almost uniform around the aerofoil, except in the shock region and at the point where turbulence transition was fixed. The grid convergence index indicates good convergence for lift but only fair convergence for moment and drag and also confirms that drag calculations are more sensitive to numerical error. © 1997 by John Wiley and Sons, Ltd.
    Additional Material: 15 Ill.
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  • 10
    Electronic Resource
    Electronic Resource
    Clinical potential of microchip capillary electrophoresis systems (1997)
    Weinheim : Wiley-Blackwell
    Electrophoresis 18 (1997), S. 1733-1741 
    Details
    ISSN: 0173-0835
    Keywords: Serum immunoassay ; DNA analysis ; Microfluidics ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Clinical interest in the use of capillary electrophoresis (CE) has recently been extended to the microchip environment. Clinical analyses demand careful handling of complex samples that are often limited in quantity and in concentration. The integrated sample handling and analysis capabilities of microchip substrates thus seem ideally suited to clinical applications. This review surveys the development of sample handling (injection, mixing, and reaction) and separation elements on-chip. The integration of these elements to create a variety of clinical analyzers has been demonstrated. The application of microchip CE systems to human serum protein analysis, immunoassay, and DNA studies is reviewed, along with various other clinical applications. In addition, the clinical potential of the lab-on-a-chip concept is discussed.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150181006
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