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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 22 (1999), S. 133-138 
    ISSN: 1476-5535
    Keywords: Keywords: Salvia miltiorrhiza; Lamiaceae; Agrobacterium rhizogenes; hairy roots; lithospermic acid B; rosmarinic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Hairy root cultures of Salvia miltiorrhiza were established by infecting sterile plantlets with Agrobacterium rhizogenes ATCC 15834, and the transformation was proved by direct detection of the inserted T-DNA by the polymerase chain reaction. As determined by HPLC, these hairy root cultures had the ability to produce lithospermic acid B (LAB), rosmarinic acid (RA) and other related phenolic compounds, the water-soluble active components of the plant. The effect of five different basal media, MS, MS-NH〈INF〉4〈/INF〉 (MS without ammonium nitrate), B5, WPM and 6,7-V on the root growth and phenolic compound production was studied. It was found that MS-NH〈INF〉4〈/INF〉 and 6,7-V media were superior to MS, B5 and WPM media in terms of both root growth and phenolic compound production. The time course of biomass accumulation and phenolic compound formation was also examined in the culture using MS-NH〈INF〉4〈/INF〉medium. During cultivation, the content of RA in the roots was stable being approximately 0.48% of dry weight while the content of LAB fluctuated between 0.73% and 1.61% of dry weight, and decreased gradually at the stationary phase of growth. The highest production of LAB and RA was about 64 mg L−1 and 23 mg L−1, respectively.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of applied electrochemistry 28 (1998), S. 1413-1418 
    ISSN: 1572-8838
    Keywords: energy conversion ; hydrogen purification ; hydrogen/oxygen gas mixture ; PEMFC ; solid polymer electrolyte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Notes: Abstract The electrochemical behaviour of a hydrogen/oxygen gas mixture at platinum electrodes in contact with a solid polymer electrolyte (Nafion 115) was studied in two modes: hydrogen enrichment and fuel cell. In the hydrogen enrichment (purification) mode, i/V(V being the applied voltage) characteristics were identified for the electrochemical cell arranged so that the gas mixture with varying ratios which contacts the anode was purified to pure hydrogen gas that was released at the cathode. In the fuel cell mode, V/i (V being the terminal voltage) characteristics were determined for the cell which utilized the gas mixture with varying ratio at the anode and pure oxygen at the cathode. The effect of the decrease in the mole fraction of hydrogen (χH2) on the current density or the terminal voltage was particularly severe for χH2〈0.6. The drop was analysed employing terms such as oxygen reduction current, oxygen adsorption and the change of e.m.f. (electromotive force) due to the decrease in the partial pressure of the hydrogen gas.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Advances in computational mathematics 6 (1996), S. 325-331 
    ISSN: 1572-9044
    Keywords: finite-difference ; partial differential equation ; fully-implicit method ; plastic forming ; binder removal ; ceramics ; 65M10
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract A mathematical model is developed to quantify the degradation of binder used for plastic forming of ceramic components and the mass transport of the degradation products. A fully-implicit finite-difference method is used to obtain numerical solutions of a non-linear differential equation governing the degradation rate, diffusion coefficient and concentration. It is able to predict the critical linear heating rate below which defects due to boiling can be avoided. Examples are given to illustrate the use of the model to investigate the effect of ceramic powder, supporting powder bed and binder properties on the critical heating rate.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Zeitschrift für angewandte Mathematik und Physik 47 (1996), S. 527-538 
    ISSN: 1420-9039
    Keywords: 35K50 ; 35B20 ; 35B30 ; 35B40 ; Heat conduction ; continuous dependence ; decay estimate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics , Physics
    Notes: Abstract Using an energy method we investigate the decay of end effects for a generalized heat conduction problem defined on a semi-infinite cylindrical region. With homogeneous Dirichlet conditions on the lateral surface of the cylinder it is shown that solutions either grow exponentially or decay exponentially in the distance from the finite end of the cylinder. The effect of perturbing the equation parameters is also investigated.
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  • 5
    ISSN: 1432-0649
    Keywords: PACS: 42.60; 42.55
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: 00 operation have been investigated under different pump light distributions that have been formed by a virtual-point-source pumping system. Compared with a central uniform distribution, a centrally depressed distribution is found to have a lower thermal lensing effect and a higher optical-to-optical efficiency for operations performed using the same resonator parameters or within a similar stable range, and the improvement in optical-to-optical efficiency is explained as a result of a reduction in thermally induced aberrations.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of environmental contamination and toxicology 63 (1999), S. 633-638 
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Algorithmica 23 (1999), S. 261-275 
    ISSN: 1432-0541
    Keywords: Key words. Difference constraints, Incremental algorithm, Linear constraints, Shortest-path problem, Dynamic negative cycle.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Mathematics
    Notes: Abstract. Difference constraints systems consisting of inequalities of the form x i - x j $ \leq $ b i,j occur in many applications, most notably those involving temporal reasoning. Often, it is necessary to maintain a solution to such a system as constraints are added, modified, and deleted. Existing algorithms handle modifications by solving the resulting system anew each time, which is inefficient. The best known algorithm to determine if a system of difference constraints is feasible (i.e., if it has a solution) and to compute a solution runs in Θ (mn) time, where n is the number of variables and m is the number of constraints. This paper presents a new efficient incremental algorithm for maintaining a solution to a system of difference constraints. As constraints are added, modified, or deleted, the algorithm determines if the new system is feasible and updates its solution. When the system becomes infeasible, the algorithm continues to process changes until it becomes feasible again, at which point a feasible solution will be produced. The algorithm processes the addition of a constraint in time O(m + n log n) and the removal of a constraint in constant time when the original system is feasible. More precisely, additions are processed in time O( || Δ || + |Δ| log|Δ| ) , where |Δ| is the number of variables whose values are changed to compute the new feasible solution, and || Δ || is the number of constraints involving the variables whose values are changed. When the original system is infeasible, the algorithm processes any change in O(m + n log n) amortized time. The new algorithm can also be used to check for the existence of negative cycles in dynamic graphs.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    International journal of biometeorology 42 (1999), S. 153-157 
    ISSN: 1432-1254
    Keywords: Key words Albedo ; Phenology ; Solar zenith angle ; Prairie grassland ; Agricultural crops
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography , Physics
    Notes: Abstract  The albedo of land surfaces is strongly affected by transient surface conditions. For a vegetated surface, albedo can change with soil moisture, fractional canopy cover, and plant phenology as well as solar zenith angle (θs). In this study, the relationships between albedo and plant phenology in prairie grassland and agricultural crops were examined by removing the effect of variations in θs. Albedos were evaluated at a constant θs, which was assigned to be 20° in this study. For days with a minimum θs larger than 20°, a polynomial function that relates albedos to θs was derived and applied to estimate the albedos at 20°. After the removal of θs variations, the observed albedos of grassland at the Konza Prairie showed a linearly decreasing trend from spring to winter due to prairie phenology. The observed albedo of a maize field showed a clear increase from seedling to peak green stage, except when drought caused a decrease in near-infrared reflectance. The observed albedos of a winter wheat field showed an uninterrupted decrease from peak green to harvesting stage.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 30 (1996), S. 273-278 
    ISSN: 1432-0983
    Keywords: Key wordsSaccharomyces cerevisiae ; Ribosomal protein gene ; RPL19A ; RPL19B
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A second copy of the Saccharomyces cerevisiae ribosomal protein YL19 gene was isolated through the use of the RPL19A gene as a probe. The nucleotide sequence of the gene, RPL19B, was determined. RPL19B contains an intron of 384 nucleotides located near its 5′-end. The coding regions of the two yeast genes, RPL19A and RPL19B, differ in only 34 nucleotides, none of which lead to changes in the amino-acid sequences of the predicted protein of 189 amino acids. RPL19B is also closely linked to a mitochondrial ADP/ATP carrier protein gene AAC2. Yeast cells containing disruption of either RPL19A or RPL19B formed smaller colonies than wild-type strains; however, simultaneous deletion of both genes is lethal.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Journal of comparative physiology 182 (1998), S. 805-815 
    ISSN: 1432-1351
    Keywords: Key words Hearing ; Sound localization ; Ear ; Eighth nerve ; Fish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract This paper reports on directional response properties of saccular afferents of the sleeper goby, Dormitator latifrons, to 100-Hz acoustic particle motions with a focus on testing the hypothesis that the response directionality of a fish's auditory afferents derives from the morphological polarity of sensory hair cells in the otolithic organs. Spontaneous rates (SR) and best sensitivities (BS) of saccular afferents ranged from 0 to 162 spikes/sec and from 0.2- to 100-nm RMS displacement. SR did not vary with BS. Most saccular afferents were phase-locked to sinusoidal stimulation and had sustained temporal response patterns with some adaptation. All saccular afferents were directionally sensitive to the stimulus, and the sharpness of directional response curves was determined by a directionality index (DI). The DI ranged from 0.64 to 1.50 (mean=1.02, SE=0.02, n=100) and gradually decreased with stimulus level throughout afferents' response dynamic range. Many afferents had approximately symmetric directional response curves relative to their best response axes (BRA). BRA of most afferents remained constant with stimulus level. The BRA distribution had a peak along an axis that correlates closely with the morphological polarity of saccular hair cells. Therefore, our results strongly support the hypothesis.
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