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  • Mathematics and Statistics  (1)
  • fission yeast  (1)
  • Cell & Developmental Biology
  • Electron microscopy
  • Wiley-Blackwell  (2)
  • American Institute of Physics
  • 1995-1999  (2)
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  • Wiley-Blackwell  (2)
  • American Institute of Physics
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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Numerical Methods for Partial Differential Equations 11 (1995), S. 423-444 
    ISSN: 0749-159X
    Keywords: Mathematics and Statistics ; Numerical Methods
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics
    Notes: This article presents a numerical study of a spectral problem that models the vibrations of a solid-fluid structure. It is a quadratic eigenvalue problem involving incompressible Stokes equations. In its numerical approximation we use Lagrange finite elements. To approximate the velocity, degree 2 polynomials on triangles are used, and for the pressure, degree 1 polynomials. The numerical results obtained confirm the theory, as they show in particular that the known theoretical bound for the maximum number of nonreal eigenvalues admitted by such a system is optimal. The results also take account of the dependence of vibration frequencies with respect to determined physical parameters, which have a bearing on the model. © 1995 John Wiley & Sons, Inc.
    Additional Material: 2 Ill.
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  • 2
    ISSN: 0749-503X
    Keywords: fission yeast ; dominant genetics ; cell wall regulation ; Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: From a Schizosaccharomyces pombe genomic library we have isolated the gene cwl1+ that causes cell lysis when it is overexpressed in the absence of an osmotic stabilizer. Southern hybridization showed that cwl1+ exists as a single copy in the S. pombe genome. The cwl1+ gene nucleotide sequence revealed a putative open reading frame of 924 bp encoding a polypeptide of 308 amino acids with a calculated Mr of 27 000. The cwl1+ DNA hybridizes to a major RNA transcript of 1·5 kb whose 5′ end maps at a position 452 bp upstream from the predicted translation start. Comparison of the amino acid sequence with those included in the current databases, showed no significant similarity to any known sequences. Cells overexpressing the cwl1+ gene under the control of the S. pombe nmt inducible promoter displayed a reduced cell wall content, were unable to separate after division and lysed drastically in the absence of osmotic stabilizer. Disruption of the cwl1+ gene caused no noticeable phenotype. The sequence has been deposited in the EMBL data library under Accession Number X9445.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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