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  • Articles  (61)
  • 1995-1999  (52)
  • 1965-1969  (9)
  • Biology  (31)
  • Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics  (30)
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  • Articles  (61)
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  • 1
    Electronic Resource
    Electronic Resource
    Insertion of EspD into epithelial target cell membranes by infecting enteropathogenic Escherichia coli (1999)
    Oxford BSL : Blackwell Science Ltd
    Molecular microbiology 31 (1999), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Diffusely adhering Escherichia coli (DAEC) strains have been implicated in epidemiological studies as a cause of diarrhoea in children. However, the molecular interactions of these pathogens with target cells have remained largely obscure. We found that some DAEC strains contain homologues of the locus of enterocyte effacement (LEE) pathogenicity island and secrete EspA, EspB and EspD proteins necessary for the formation of the attaching and effacing (A/E) lesions. To characterize the function of the EspD protein further, we cloned and sequenced the espD genes of two DA-EPEC strains and compared their deduced amino-acid sequences with known EspD sequences. A pattern of two conserved transmembrane regions and one conserved coiled-coil region is predicted in EspD and also in the type III system secreted proteins YopB, PopB, IpaB and SipB of Yersinia, Pseudomonas, Shigella and Salmonella respectively. The EspD protein is inserted into a trypsin-sensitive location in the HeLa cell membrane at sites of bacterial contact, but is not translocated into the cytoplasm. Secretion of EspD increases upon contact with host cells. We propose that the membrane-located EspD protein is part of the translocation apparatus for Esp proteins into the target host cell performing functions similar to YopB in Yersinia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.1999.01303.x
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  • 2
    Electronic Resource
    Electronic Resource
    Processing of the AIDA-I precursor: removal of AIDAC and evidence for the outer membrane anchoring as a β-barrel structure (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 22 (1996), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The AIDA-I adhesin known to be responsible for the diffuse adherence (DA) phenotype of the diarrhoea-genie Escherichia coli (DAEC) strain 2787 has been shown previously to be synthesized as a precursor protein and to undergo additional C-terminal processing. Here, the C-terminal processing of the AIDA-I precursor and the outer membrane topology of the cleaved C-terminal fragment, AIDAC, were investigated. By isolation of the cleaved AIDAC fragment and N-terminal sequencing, the C-terminal cleavage site was identified between Ser-846 and Ala-847 thereby indicating a molecular mass of 47.5 kDa for AIDAC. The correct processing to AIDA-I and AIDAC in OmpT, OmpP and DegP protease-deficient E. coli strains as well as in avirulent salmonellae and shigellae points to an autocatalytic cleavage mechanism. The cleaved AIDAC was localized in the outer membrane. A leader sequence-AIDAC fusion was efficiently routed to the outer membrane. Analysis by protease digestion, secondary-structure prediction and modelling, by comparison with structurally related bacterial proteins like the lgA1 protease from neisseria, the vacuolating toxin from Helicobacter pylori, and the VirG protein of Shigella flexneri, strongly indicates that AIDAC is present in the outer membrane as a β-barrel structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1996.tb02653.x
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  • 3
    Electronic Resource
    Electronic Resource
    Prevention of Cholestanol-induced Cholelithiasis by Neomycin (1965)
    [s.l.] : Nature Publishing Group
    Nature 208 (1965), S. 1226-1227 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Since a major step in the formation of gall-stones appears to be the bacterial 7-dehydroxylation of allocholic acid, it is reasonable to assume that the reduction or elimination of the bacteria responsible for the conversion might interfere with gall-stone formation. Because of its well-known ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/2081226a0
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  • 4
    Electronic Resource
    Electronic Resource
    Discovery of hardest known oxide (1996)
    [s.l.] : Nature Publishing Group
    Nature 383 (1996), S. 401-401 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SIR á€" Microhardness measurements on synthesized samples of stishovite, a high-pressure phase of silica, show that it is the hardest oxide yet discovered. Among polycrystalline materials, its hardness (33 gigapascals, GPa) rivals those of the hardest materials. Despite many ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/383401a0
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  • 5
    Electronic Resource
    Electronic Resource
    RecA-dependent viral burst in bacterial colonies during the entry into stationary phase (1999)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 170 (1999), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: We have explored the nature of the sudden viral amplification observed during the ageing of P22-infected lysogenic colonies of Salmonella typhimurium [Ramı́rez, E, and Villaverde, A. (1997) Gene 202, 147–149]. By a comparative analysis of the wild-type P22 and a P22 integration mutant, it has been shown that the conditions promoting prophage induction occur in only a small portion of the bacterial population and briefly during the transition between the exponential growth and the stationary phase. The viral burst is RecA-dependent and cannot be reproduced in continuous culture by a mere decrease of the growth rate. This suggests that the limited viral propagation in colonies is probably linked to heterogeneous physiological conditions within colonial populations, distinct from those of the homogeneous liquid cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1999.tb13389.x
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  • 6
    Electronic Resource
    Electronic Resource
    Film formation of latex dispersions observed with evanescent dynamic light scattering (1997)
    Springer
    Colloid & polymer science 104 (1997), S. 191-193 
    Details
    ISSN: 1435-1536
    Keywords: Evanescent dynamic light scattering ; colloidal dispersions ; film formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract We have applied evanescent dynamic light scattering (DLS) to study the film formation of polymer latex dispersions. A dynamical glass transition is observed. When the particles fuse, the scattering rate decreases. After fusion, a new fast process (τ r 1 ms) is observed, which is not present in bulk DLS data. We attribute the process to discontinuous relaxation of surface induced stresses (transient micro-cracks).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01182447
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  • 7
    Electronic Resource
    Electronic Resource
    Automatisches Herstellen von englumigen Glaskapillaren, Glasfäden und Mikropipetten (1966)
    Springer
    Naturwissenschaften 53 (1966), S. 582-582 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00600532
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  • 8
    Electronic Resource
    Electronic Resource
    Structural Investigation of Milled α-Fe and Barium Ferrite Nano-Mixtures (1999)
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 312-314 (July 1999), p. 197-202 
    Details
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/02/04/transtech_doi~10.4028%252Fwww.scientific.net%252FMSF.312-314.197.pdf
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  • 9
    Electronic Resource
    Electronic Resource
    Processing of antennular input in the brain of the spiny lobster, Panulirus argus (1996)
    Springer
    Journal of comparative physiology 178 (1996), S. 605-628 
    Details
    ISSN: 1432-1351
    Keywords: Crustacean ; Olfaction ; Mechanoreception ; Local interneurons ; Projection neurons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Neurons in the olfactory deutocerebrum of the spiny lobster, Panulirus argus, were recorded intracellularly and filled with biocytin. Recorded neurons arborized in the olfactory lobe (OL), a glomerular neuropil innervated by olfactory and some presumptive mechanosensory antennular afferents. The neurons responded to chemosensory input from the lateral antennular flagellum bearing the olfactory sensilla but not the medial flagellum bearing many non-olfactory chemosensory sensilla. Many neurons received additional mechanosensory input. Thus the OL integrates specifically olfactory with mechanosensory input. OL neurons had multiglomerular arborizations restricted to one or two of the three horizontal layers of the columnar glomeruli. OL local interneurons comprised “core” neurons with tree-like neurites and terminals in the base of the glomeruli and “rim” neurons with neurites surrounding the OL and terminals in the cap/subcap. The somata of OL local interneurons lay in the medial soma cluster (100000 somata). OL projection neurons arborized in the base of the glomeruli and ascended via the olfactory glomerular tract to the lateral protocerebrum. A parallel projection pathway is constituted by projection neurons of the accessory lobe, a glomerular neuropil without afferent innervation but intimate links to the OL. The projection neuron somata constituted the lateral soma cluster (200000 somata).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00227375
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  • 10
    Electronic Resource
    Electronic Resource
    Processing of antennular input in the brain of the spiny lobster, Panulirus argus (1996)
    Springer
    Journal of comparative physiology 178 (1996), S. 579-604 
    Details
    ISSN: 1432-1351
    Keywords: Crustacean ; Chemoreception ; Mechanoreception ; Deutocerebrum ; Motoneurons ; Projection neurons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Neurons in the brain of the spiny lobster that respond to chemical and mechanical stimulation of the antennule (antenna I) were recorded and stained intracellularly. Described here are neurons that do not arborize in the olfactory and accessory lobes of the deutocerebrum, but rather primarily target the lateral and/or the median antennular neuropils of the deutocerebrum. Some of the neurons also extend into the antennal and tegumentary neuropils of the tritocerebrum and the neuropils of the median protocerebrum. Included are antennular sensory afferents, antennular motoneurons, projection neurons descending from the central brain, projection neurons ascending from the central brain and projection neurons descending from the eyestalk ganglia. Collectively, these neurons consitutute a novel antennular sensory pathway that is parallel to and independent of the antennular olfactory pathway. The novel pathway integrates mechanosensory and non-olfactory chemosensory information in the lateral and/or the median antennular neuropils, which also serve as lower motor centers of the antennule. Division of the arthropod deutocerebrum into two, functionally distinct chemosensory pathways may reflect differences in how chemosensory information is processed that is fundamental to understanding the origin of the sense of smell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00227374
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