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Processing of antennular input in the brain of the spiny lobster, Panulirus argus

I. Non-olfactory chemosensory and mechanosensory pathway of the lateral and median antennular neuropils

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Abstract

Neurons in the brain of the spiny lobster that respond to chemical and mechanical stimulation of the antennule (antenna I) were recorded and stained intracellularly. Described here are neurons that do not arborize in the olfactory and accessory lobes of the deutocerebrum, but rather primarily target the lateral and/or the median antennular neuropils of the deutocerebrum. Some of the neurons also extend into the antennal and tegumentary neuropils of the tritocerebrum and the neuropils of the median protocerebrum. Included are antennular sensory afferents, antennular motoneurons, projection neurons descending from the central brain, projection neurons ascending from the central brain and projection neurons descending from the eyestalk ganglia. Collectively, these neurons consitutute a novel antennular sensory pathway that is parallel to and independent of the antennular olfactory pathway. The novel pathway integrates mechanosensory and non-olfactory chemosensory information in the lateral and/or the median antennular neuropils, which also serve as lower motor centers of the antennule. Division of the arthropod deutocerebrum into two, functionally distinct chemosensory pathways may reflect differences in how chemosensory information is processed that is fundamental to understanding the origin of the sense of smell.

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Abbreviations

AC :

anterior cluster (cluster 6, 7)

AL :

ccessory lobe

aMC :

anterior subcluster of medial cluster (cluster 9)

AnN :

antenna II (antennal) neuropil

A INv:

main antenna I (antennular) nerve

A INM:

antenna I (antennular) motor nerve

A IINv:

main antenna II (antennal) nerve

A IINM:

antenna II (antennal) motor nerve

DCN :

deutocerbral commissure neuropil

dDLC :

dorsal subcluster of dorsal lateral cluster (cluster 15)

dDUMC :

dorsal subcluster of dorsal unpaired median cluster (cluster 17)

DUGC :

dorsal unpaired globuli cell cluster

LAN :

lateral antenna I (antennular) neuropil

IDUMC :

lateral subcluster of dorsal unpaired median cluster (cluster 16)

LF :

lateral flagellum of antenna I (antennule)

MAN :

median antenna I (antennular) neuropil

mDUMC :

median subcluster of dorsal unpaired median cluster (cluster 17)

MF :

medial flagellum of antenna I (antennule)

MPN :

anterior and posterior median protocerebral neuropils

OC :

oesophageal connective

OCM :

oculomotor nerve

OL :

olfactory lobe

PT :

protocerebral tract

TN :

tegumentary neuropil

TNv :

tegumentary nerve

vDLC :

ventral subcluster of dorsal lateral cluster (cluster 14)

VPALC :

ventral paired anterolateral cluster (cluster 8)

VUMC :

ventral unpaired median cluster (cluster 13)

AMP :

adenosine monophosphate

ASC :

L-ascorbic acid

ASW :

artificial sea water

BET :

betaine HCl

M1 :

mixture 1 (TAU, BET, ASC, NH4)

M2 :

mixture 2 (SUC, NIC, AMP, TMA)

M3 :

mixture 3 (M1, M2, PRO)

NH 4 :

NH4Cl

NIC :

nicotinic acid

PRO :

L-proline

SUC :

sucrose

TAU :

taurine

TM :

TetraMarin extract

TMA :

trimethylamine HCl

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Authors and Affiliations

  1. Institut für Biologie, TU Berlin, Franklinstrasse 28/29, D-10587, Berlin, Germany

    M. Schmidt

  2. Whitney Laboratory, University of Florida, 32086, St. Augustine, FL, USA

    M. Schmidt & B. W. Ache

  3. Departments of Zoology and Neuroscience, University of Florida, 32086, St. Augustine, FL, USA

    B. W. Ache

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Schmidt, M., Ache, B.W. Processing of antennular input in the brain of the spiny lobster, Panulirus argus . J Comp Physiol A 178, 579–604 (1996). https://doi.org/10.1007/BF00227374

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