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  • 1
    Electronic Resource
    Electronic Resource
    Kinetics of exchange of 4-picoline 1-oxide in nickel(II)- and cobalt(II)-.beta.-diketone complexes (1970)
    s.l. : American Chemical Society
    Inorganic chemistry 9 (1970), S. 2592-2594 
    Details
    ISSN: 1520-510X
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ic50093a045
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  • 2
    Electronic Resource
    Electronic Resource
    Genetic rearrangements in the tyrB-uvrA region of the enterobacterial chromosome: a potential cause for different class B acid phosphatase regulation in Salmonella enterica and Escherichia coli (1999)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 181 (1999), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Unlike in Escherichia coli, in Salmonella enterica production of class B acid phosphatase (AphA) was detectable also in cells growing in the presence of glucose. Characterization of the aphA locus from a S. enterica ser. typhi strain showed that the aphA determinant is very similar to the E. coli homolog, and that its chromosomal location between the highly conserved tyrB and uvrA genes is retained. However, the aphA flanking regions were found to be markedly different in the two species, either between tyrB and aphA or between aphA and uvrA. The differences in the aphA 5′-flanking region, which in S. enterica is considerably shorter than in E. coli (183 vs. 1121 bp) and includes potential promoter sequences not present in E. coli, could be responsible for the different regulation of class B acid phosphatase observed in the two species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1999.tb08821.x
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  • 3
    Electronic Resource
    Electronic Resource
    Identification of the gene (aphA) encoding the class B acid phosphatase/phosphotransferase of Escherichia coli MG1655 and characterization of its product (1997)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 146 (1997), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: An open reading frame located in the tyrB-uvrA intergenic region of the Escherichia coli MG1655 chromosome was identified as encoding the class B acid phosphatase of this species on the basis of cloning and expression experiments. A protocol for purification of the enzyme (named AphA) was developed, and its properties were analyzed. The enzyme is a 100-kDa homotetrameric protein which apparently requires a metal co-factor for activity. Similarly to other bacterial class B acid phosphatases, it is able to dephosphorylate several organic phosphomonoesters as well as to catalyze the transfer of low-energy phosphate groups from phosphomonoesters to hydroxyl groups of various organic compounds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1997.tb10192.x
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  • 4
    Electronic Resource
    Electronic Resource
    Retinoid Signaling in Vertebrate Limb Development (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 785 (1996), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1996.tb56237.x
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  • 5
    Electronic Resource
    Electronic Resource
    The use of Escherichia coli bearing a phoN gene for the removal of uranium and nickel from aqueous flows (1998)
    Springer
    Applied microbiology and biotechnology 50 (1998), S. 266-272 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A Citrobacter sp. originally isolated from metal-polluted soil accumulates heavy metals via metal-phosphate deposition utilizing inorganic phosphate liberated via PhoN phosphatase activity. Further strain development was limited by the non-transformability of this environmental isolate. Recombinant Escherichia coli DH5α bearing cloned phoN or the related phoC acquired metal-accumulating ability, which was compared with that of the Citrobacter sp. with respect to removal of uranyl ion (UO2 2+) from dilute aqueous flows and its deposition in the form of polycrystalline hydrogen uranyl phosphate (HUO2PO4). Subsequently, HUO2PO4-laden cells removed Ni2+ from dilute aqueous flows via intercalation of Ni2+ into the HUO2PO4 lattice. Despite comparable acid phosphatase activity in all three strains, the E. coli DH5α (phoN) construct was superior to Citrobacter N14 in both uranyl and nickel accumulation, while the E. coli DH5α (phoC) construct was greatly inferior in both respects. Expression of phosphatase activity alone is not the only factor that permits efficient and prolonged metal phosphate accumulation, and the data highlight possible differences in the PhoN and PhoC phosphatases, which are otherwise considered to be related in many respects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051288
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  • 6
    Electronic Resource
    Electronic Resource
    Bacterial nonspecific acid phosphohydrolases: physiology, evolution and use as tools in microbial biotechnology (1998)
    Springer
    Cellular and molecular life sciences 54 (1998), S. 833-850 
    Details
    ISSN: 1420-9071
    Keywords: Key words. Acid phosphohydrolases; bacteria; genetics; physiology; molecular evolution; microbial biotechnology.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Bacterial nonspecific acid phosphohydrolases (NSAPs) are secreted enzymes, produced as soluble periplasmic proteins or as membrane-bound lipoproteins, that are usually able to dephosphorylate a broad array of structurally unrelated substrates and exhibit optimal catalytic activity at acidic to neutral pH values. Bacterial NSAPs are monomeric or oligomeric proteins containing polypeptide components with an M r of 25 – 30 kDa. On the basis of amino acid sequence relatedness, three different molecular families of NSAPs can be distinguished, indicated as molecular class A, B and C, respectively. Members of each class share some common biophysical and functional features, but may also exhibit functional differences. NSAPs have been detected in several microbial taxa, and enzymes of different classes can be produced by the same bacterial species. Structural and phyletic relationships exist among the various bacterial NSAPs and some other bacterial and eucaryotic phosphohydrolases. Current knowledge on bacterial NSAPs is reviewed, together with analytical tools that may be useful for their characterization. An overview is also presented concerning the use of bacterial NSAPs in biotechnology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s000180050212
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  • 7
    Electronic Resource
    Electronic Resource
    A Monte Carlo method for Bayesian analysis of linkage between single markers and quantitative trait loci. I. Methodology (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 1161-1166 
    Details
    ISSN: 1432-2242
    Keywords: Key words Linkage analysis ; Bayesian method ; Markov chain Monte Carlo ; Quantitative-trait loci
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A Bayesian approach to the statistical mapping of Quantitative Trait Loci (QTLs) using single markers was implemented via Markov Chain Monte Carlo (MCMC) algorithms for parameter estimation and hypothesis testing. Parameter estimators were marginal posterior means computed using a Gibbs sampler with data augmentation. Variables sampled included the augmented data (marker-QTL genotypes, polygenic effects), an indicator variable for linkage, and the parameters (allele frequency, QTL substitution effect, recombination rate, polygenic and residual variances). Several MCMC algorithms were derived for computing Bayesian tests of linkage, which consisted of the marginal posterior probability of linkage and the marginal likelihood of the QTL variance associated with the marker.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050351
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  • 8
    Electronic Resource
    Electronic Resource
    A Monte Carlo method for Bayesian analysis of linkage between single markers and quantitative trait loci. II. A simulation study (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 1167-1174 
    Details
    ISSN: 1432-2242
    Keywords: Key words Linkage analysis ; Bayesian method ; Markov chain Monte Carlo ; Quantitative-trait loci ; Simulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A Bayesian approach to the statistical mapping of Quantitative Trait Loci (QTLs) using single markers was implemented via Markov Chain Monte Carlo (MCMC) algorithms for parameter estimation and hypothesis testing. Parameters were estimated by marginal posterior means computed with a Gibbs sampler with data augmentation. Variables sampled included the augmented data (marker-QTL genotypes, polygenic effects), the event of linkage or nonlinkage, and the parameters (allele frequencies, QTL substitution effect, recombination rate, polygenic and residual variances). The analysis was evaluated empirically via application to simulated granddaughter designs consisting of 2000 sons, 20 related sires and their ancestors. Results obtained in this study and preliminary work on multiple linked markers and multiple QTLs support the usefulness of the Bayesian method for the statistical mapping of QTLs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050352
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  • 9
    Electronic Resource
    Electronic Resource
    A Monte Carlo method for Bayesian analysis of linkage between single markers and quantitative trait loci. II. A simulation study (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 1167-1174 
    Details
    ISSN: 1432-2242
    Keywords: Linkage analysis ; Bayesian method ; Markov chain Monte Carlo ; Quantitative-trait loci ; Simulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A Bayesian approach to the statistical mapping of Quantitative Trait Loci (QTLs) using single markers was implemented via Markov Chain Monte Carlo (MCMC) algorithms for parameter estimation and hypothesis testing. Parameters were estimated by marginal posterior means computed with a Gibbs sampler with data augmentation. Variables sampled included the augmented data (marker-QTL genotypes, polygenic effects), the event of linkage or nonlinkage, and the parameters (allele frequencies, QTL substitution effect, recombination rate, polygenic and residual variances). The analysis was evaluated empirically via application to simulated granddaughter designs consisting of 2000 sons, 20 related sires and their ancestors. Results obtained in this study and preliminary work on multiple linked markers and multiple QTLs support the usefulness of the Bayesian method for the statistical mapping of QTLs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00230142
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  • 10
    Electronic Resource
    Electronic Resource
    A Monte Carlo method for Bayesian analysis of linkage between single markers and quantitative trait loci. I. Methodology (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 1161-1166 
    Details
    ISSN: 1432-2242
    Keywords: Linkage analysis ; Bayesian method ; Markov chain Monte Carlo ; Quantitative-trait loci
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A Bayesian approach to the statistical mapping of Quantitative Trait Loci (QTLs) using single markers was implemented via Markov Chain Monte Carlo (MCMC) algorithms for parameter estimation and hypothesis testing. Parameter estimators were marginal posterior means computed using a Gibbs sampler with data augmentation. Variables sampled included the augmented data (marker-QTL genotypes, polygenic effects), an indicator variable for linkage, and the parameters (allele frequency, QTL substitution effect, recombination rate, polygenic and residual variances). Several MCMC algorithms were derived for computing Bayesian tests of linkage, which consisted of the marginal posterior probability of linkage and the marginal likelihood of the QTL variance associated with the marker.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00230141
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