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  • Springer  (223)
  • Blackwell Publishing Ltd  (27)
  • 1995-1999  (213)
  • 1975-1979  (26)
  • 1970-1974  (11)
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  • 11
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 221 (1974), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 12
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY– 392 samples of precooked frozen shrimps from 2 Chilean industries (A and B) were analyzed for total bacterial count, conforms and enterococci throughout a period of 8 months. 1-lb samples of breaded shrimp were received directly from the manufacturers after a freezing period of 10 days at —18δC. Total bacterial count ranged from 104 to 105 organisms perg. Conforms were absent in 65% of the samples from A, and in 40% of those from 8; 89.6% of the samples from A and 50.1% of those from B were bacteriologically acceptable considering a limit of not more than 50 coliforms per g. 98% of the frozen shrimp samples belonging to A contained enterococci, as did 66% of the samples from B. Smaller percentages (17% for A and 54% for B) of acceptable samples are obtained from both industries when 100 enterococci per g is considered as the limit. During the period of observation some sanitary measures were adopted and subsequent coliform counts improved. In plant A working conditions are better and the understanding of bacteriological grounds for the proper handling of food materials has led to the elaboration of a product of consistently better quality. Enterococci counts are in contradiction with coliform counts, since the low-level coliform samples are rejected on the basis of their enterococcal content. In plant B there is a better correlation between coliform and enterococcal counts. Though not investigated, this may be related to the precooking system employed: steaming in an enclosed conveyor in A versus immersion in boiling sea-water in B.
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  • 13
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 47 (1995), S. 0 
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A scanning electron microscopy study showed the presence of large pores in the endocardium of the sinus venosus of the dogfish Scyliorhinus canicula. The pores were always found on large bundles which protruded into the cardiac lumen. The bundles were mainly constituted of granule-containing nerve fibres. The average diameter of the pores was 3.2 μm (range=1.5-5.0μm), and their density, was about 822 pores mm−2. Endocardial pores were absent in other areas of the sinus venosus, but they were observed on the little bundles of granulated nerve fibres which were scattered throughout the atrium. The existence of large endocardial pores associated with bundles of granulated nerve fibres supports the hypothesis for the neuroendocrine nature of the elasmobranch sinus venosus wall.
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  • 14
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Ground water monitoring & remediation 15 (1995), S. 0 
    ISSN: 1745-6592
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: A two-dimensional mathematical model for acid leaching of copper from mine tailings using injection and pumping wells is presented. The model considers the transport mechanisms of convection and dispersion with reactions that govern the fates of copper and acid. Reactions considered are acid neutralization, copper solubilization, and copper adsorption. The results of acid leaching experiments in laboratory columns (one- dimensional) and in the field at a tailings impoundment are presented. The proposed model was validated for a one-dimensional experiment, and the model parameter fitting was found to be adequate in terms of the field experiment.
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  • 15
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 283 (1977), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
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  • 16
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 35 (1970), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY– 392 samples of precooked frozen shrimp from two Chilean manufacturers, A and B, were quantitatively examined for the presence of Staphylococcus aureus by direct plating on Difco mannitol salt agar (MSA, 10% NaCI). 140 samples (35.7%) were found to contain Staphylococci but only half of these had counts of over 100,82.4% remaining within the acceptable limit. Frozen shrimps are prone to contamination by Staphylococcus during processing, especially if hand-processed, but these results show that it is possible to obtain a good-quality product when stringent sanitary measures are observed. MSA was compared with Baird Parker's egg yolk medium (BPM) by plating simultaneously on it 141 samples from manufacturer B; BPM detected S. aureus in a smaller number of samples (7.8%), and gave rise to fewer colonies than MSA. BPM seemed to be inhibitory even to some Staphylococcus strains, i.e., it is unsuitable for use in these frozen foods. From 2 other manufacturers, C and D, 80 samples of frozen shrimp, together with 60 nasal swab samples from food handlers were plated to investigate some cultural characteristics of S. aureus. 57 strains of this organism were obtained, 41 belonging to shrimp samples and 16 to nasal carriers from both manufacturers. The strains were isolated and, when tested by anaerobic fermentation of mannitol, deep growth in cysteine agar, catalase and coagulase reactions, all gave positive tests. Phosphatase and DNase reactions were less constant. All the strains with 1 exception were sensitive to 8 antibiotics tested. Thus, the general properties ascribed to S. aureus species appear unaltered in frozen shrimps. 28 strains (49.1%) could be typified with the set of 21 international phages, most of them belonging to group III; only in manufacturer D strains coming from food and food handlers were phage type related. In manufacturer C most strains were untypable.
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  • 17
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 94 (1995), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The involvement of three NAD(P)+-L-glutamate dehydrogenase (GDH; EC 1.4.1.3) isoenzymes, named GDH1, GDH2 and GDH3, in the carbon and nitrogen metabolism of the green alga Chlamydomonas reinhardtü 6145c has been investigated under different environmental and stress conditions. GDH1 activity decreased, but GDH2 and GDH3 activities increased with the age of cultures. When the extracellular ammonium concentration was high, only GDH1 activity increased with growth whereas GDH2 and GDH3 remained unchanged. In the presence of L-methionine-D,L-sulfoximine (MSX), an inhibitor of L-glutamine synthetase (GS), a significant increase of GDH1 and a slight decrease in GDH3 activity was observed, whereas GDH2 did not change. A significant increase in the intracellular 2-oxoglutarate was also found upon addition of azaserine, an inhibitor of L-glutamate synthase (GOGAT) activity. However, no significant changes in GDH isoenzyme activities were observed after addition of azaserine or azaserine plus MSX, except an induction of GDH3 in the latter case. Moreover, in the presence of ethoxyzolamide (ETZ), an inhibitor of carbonic anhydrase activity, an induction of total GDH activity, mainly due to an increase in GDH1 and to a minor extent in GDH2, was observed in cells under low CO2 (0.03%). In the dark, cells showed an increase in GDH1 activity, but when acetate was present GDH1 activity was repressed. All these results taken together suggest a relationship between GDH1 and nitrogen assimilation, whereas GDH2 and GDH3 seem to be involved in the production of 2-oxoglutarate to fuel the tricarboxylic acid (TCA) cycle.
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  • 18
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Previous studies have indicated that the rate of photorespiration in C4 plants is low or negligible. In this study, wild-type and mutant leaves of the C4 plant Amaranthus edulis were treated with the glutamine synthetase inhibitor, phosphinothricin and the glycine decarboxylase inhibitor, aminoacetonitrile, at different concentrations of CO2. The time course of ammonia accumulation in leaves of the wild type was compared with a mutant lacking phosphoenolpyruvate carboxylase activity (EC 4.1.1.31), and with three different mutants that accumulated glycine. The increase in the concentration of ammonia in the leaves, stimulated by the treatments was used as a measurement of the rate of photorespiration in C4 plants.The application of glutamine and glycine maintained the rate of photorespiratory ammonia production for a longer period in the wild type, and increased the rate in a mutant lacking phosphoenolpyruvate carboxylase suggesting that there was a lack of amino donors in these plants.The calculated rate of photorespiration in Amaranthus edulis wild-type leaves when the supply of amino donors was enough to maintain the photorespiratory nitrogen flow, accounted for approximately 6% of the total net photosynthetic CO2 assimilation rate. In a mutant lacking phosphoenolpyruvate carboxylase, however, this rate increased to 48%, when glutamine was fed to the leaf, a value higher than that found in some C3 plants. In mutants of Amaranthus edulis that accumulated glycine, the rate of photorespiration was reduced to 3% of the total net CO2 assimilation rate. The rate of ammonia produced during photorespiration was 60% of the total produced by all metabolic reactions in the leaves. The data suggests that photorespiration is an active process in C4 plants, which can play an important role in photosynthetic metabolism in these plants.
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  • 19
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A prospective study was carried out to determine the prevalence rates of microsporidiosis and other enteroparasites in HIV-positive children in the Madrid area. HIV-positive pediatric patients from three hospitals were entrolled in the study. A total of 293 samples (158 stool and 127 urine) were collected from 83 children whose mean age was 6.3 years and hed a mean CD4 count of 504.7/mm3 (range 1-2,220/mm3), 48 of whom suffered diarrhea at the time of the study. Microsporidia indentification was investigated in stool and unrine samples using Weber's chromotrope-based strain, IIF and PCR species-specific tests. Enteric parasites were identified in 32.5% of the children. Cryptosporidium sp. was the most common parasite encuntered (14.4%), followed by Blastocytis sp. (9.6%) and Giardia duodenalis (8.4%). Microsoridia was only found in the stools of one child (1.2% of total and 2% of those with diarrhea) and Enterocytozoon bieneusi was demonstrated by PCR. The patient was 10 years old, Presented non-chronic diarrhea and his CD4 count was 298/mm3. These date differ from those previously reported by us in HIV-positive adults (13.9%) in the same area, although this group showed more severely depressed CD4 lymphocyte counts than children. New epidemiological studies should be carried out to elucidate whether additional risk factors exist betwen these groups.
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  • 20
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 168 (1998), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Dengue viruses are arthropod-borne, single-stranded RNA viruses. Aëdes aegypti and Aëdes albopictus are the principal vectors. In order to understand the molecular basis of dengue virus infections we explored the biochemical identity of dengue-2 (DEN-2) virus receptors in the Aëdes albopictus-derived cell line C6/36. We show here that DEN-2 interacts with two major polypeptides of 80 and 67 kDa. Polyclonal anti-C6/36 membrane antibodies block DEN-2 binding to intact C6/36 monolayers as well as to membrane extracts. Our results strongly suggest that the identified polypeptides are part of the DEN-2 receptors.
    Type of Medium: Electronic Resource
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