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  • Articles  (152)
  • Springer  (152)
  • 1995-1999  (121)
  • 1980-1984  (30)
  • 1955-1959  (1)
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  • Articles  (152)
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  • 1
    Electronic Resource
    Electronic Resource
    Analysis of segregation and aneuploidy in two reciprocal translocation carriers, t(3;9)(q26.2;q32) and t(3;9)(p25;q32), by triple-color fluorescence in situ hybridization (1999)
    Springer
    Human genetics 〈Berlin〉 105 (1999), S. 428-436 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Meiotic segregation patterns of chromosomes 3 and 9 were analyzed in sperm of two translocation carriers (t(3;9)(q26.2;q32) and t(3;9)(p25;q32)) by triple-color fluorescent in situ hybridization (FISH) with a telomeric DNA probe in addition to two centromeric probes. The frequencies of each sperm product resulting from alternate or adjacent I, adjacent II and 3:1 segregation in a t(3;9)(q26.2;q32) translocation carrier were 88.35%, 5.44% and 5.94%, respectively. On the other hand, the frequencies of each sperm product in a t(3;9)(p25;q32) translocation carrier were 89.23%, 6.02% and 4.48%, respectively. Of all the sperm products, the frequency of normal or chromosomally balanced sperm in a t(3;9)(q26.2;q32) and a t(3;9)(p25;q32) were 52.49% and 47.25%, respectively. The frequencies of each sperm product resulting from various segregations were different between both carriers and significantly deviated from the expected frequencies. Additional dual-color and triple-color FISH were performed to analyze aneuploidy rates for chromosomes 12, 17, 18, X and Y in order to detect any interchromosomal effect; no evidence of an interchromosomal effect was found.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004399900161
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  • 2
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    Analysis of segregation and aneuploidy in two reciprocal translocation carriers, t(3;9)(q26.2;q32) and t(3;9)(p25;q32), by triple-color fluorescence in situ hybridization (1999)
    Springer
    Details
    Publication Date: 1999-11-01
    Print ISSN: 0340-6717
    Electronic ISSN: 1432-1203
    Topics: Biology , Medicine
    Published by Springer
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  • 3
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    Analysis of segregation and aneuploidy in two reciprocal translocation carriers, t(3;9)(q26.2;q32) and t(3;9)(p25;q32), by triple-color fluorescence in situ hybridization (1999)
    Springer
    Details
    Publication Date: 1999-11-15
    Print ISSN: 0340-6717
    Electronic ISSN: 1432-1203
    Topics: Biology , Medicine
    Published by Springer
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    PAPER CURRENT
  • 4
    Electronic Resource
    Electronic Resource
    Thioredoxin h is one of the major proteins in rice phloem sap (1995)
    Springer
    Planta 195 (1995), S. 456-463 
    Details
    ISSN: 1432-2048
    Keywords: Oryza (phloem proteins) ; Phloem sap ; protein ; Sieve tube ; Thioredoxin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sieve tubes play important roles in the transfer of nutrients as well as signals. Hundreds of proteins were found in pure phloem sap collected from rice (Oryza sativa L. cv. Kantou) plants through the cut ends of insect stylets. These proteins may be involved in nutrient transfer and signal transduction. To characterize the nature of these proteins, the partial amino-acid sequence of a 13kDa protein, named RPP13-1, that was abundant in the pure phloem sap was determined. A cDNA clone of 687 bp, containing an open reading frame of 122 amino acids, was isolated using corresponding oligonucleotides as a probe. The deduced amino-acid sequence was very similar to that of the ubiquitous thiol redox protein, thioredoxin. The consensus sequences of thioredoxins are highly conserved. No putative signal peptide was identified. Antiserum against wheat thioredoxin h cross-reacted with RPP13-1 in the phloem sap of rice plants. RPP131 produced in Escherichia coli was reactive to antiserum against wheat thioredoxin h. Both E. coli-produced RPP13-1 and the phloem sap proteins catalyzed the reduction of the disulfide bonds of insulin in the presence of dithiothreitol. These results indicate that an active thioredoxin is a major protein translocating in rice sieve tubes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00202605
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  • 5
    Electronic Resource
    Electronic Resource
    Detection of Seven Major Evolutionary Lineages in Cyanobacteria Based on the 16S rRNA Gene Sequence Analysis with New Sequences of Five Marine Synechococcus Strains (1999)
    Springer
    Journal of molecular evolution 48 (1999), S. 723-739 
    Details
    ISSN: 1432-1432
    Keywords: Key words: Cyanobacteria —Synechococcus— Phylogeny — Ribosomal RNA — Maximum-likelihood method — Neighbor-joining method — Rate homo- and heterogeneous models
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Although molecular phylogenetic studies of cyanobacteria on the basis of the 16S rRNA gene sequence have been reported, the topologies were unstable, especially in the inner branchings. Our analysis of 16S rRNA gene phylogeny by the maximum-likelihood and neighbor-joining methods combined with rate homogeneous and heterogeneous models revealed seven major evolutionary lineages of the cyanobacteria, including prochlorophycean organisms. These seven lineages are always stable on any combination of these methods and models, fundamentally corresponding to phylogenetic relationships based on other genes, e.g., psbA, rbcL, rnpB, rpoC, and tufA. Moreover, although known genotypic and phenotypic characters sometimes appear paralleled in independent lineages, many characters are not contradictory within each group. Therefore we propose seven evolutionary groups as a working hypothesis for successive taxonomic reconstruction. New 16S rRNA sequences of five unicellular cyanobacterial strains, PCC 7001, PCC 7003, PCC 73109, PCC 7117, and PCC 7335 of Synechococcus sp., were determined in this study. Although all these strains have been assigned to ``marine clusters B and C,'' they were separated into three lineages. This suggests that the organisms classified in the genus Synechococcus evolved diversely and should be reclassified in several independent taxonomic units. Moreover, Synechococcus strains and filamentous cyanobacteria make a monophyletic group supported by a comparatively high statistical confidence value (80 to 100%) in each of the two independent lineages; therefore, these monophylies probably reflect the convergent evolution of a multicellular organization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00006517
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  • 6
    Electronic Resource
    Electronic Resource
    An age-related decrease in the concentration of insulin-like growth factor binding protein-5 in human cortical bone (1995)
    Springer
    Calcified tissue international 57 (1995), S. 206-212 
    Details
    ISSN: 1432-0827
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract The skeletal contents of insulin-like growth factor-2 (IGF-II), insulin-like growth factor binding protein-5 (IGFBP-5), and insulin-like growth factor binding protein-3 (IGFBP-3) were determined in duplicate samples of human femoral cortical bone obtained from 64 subjects (44 males and 20 females) between the ages of 20 and 64 years. The results of these quantitative measurements revealed an age-related decrease in the femoral cortical content of IGFBP-5 (r=-0.272, P=0.031) in the total population. Although the femoral cortical content of IGF-II did not show a similar decrease with age, it could be correlated to the femoral cortical content of IGFBP-5 (r=0.442, P〈0.001). In constrast, the femoral cortical content of IGFBP-3 did not decrease with age and could not be correlated to the femoral cortical contents of either IGFBP-5 or IGF-II. Comparisons of these results with previous measurements of insulin-like growth factor-1 (IGF-I) and transforming growth factor-β (TGF-β), in extracts of the same bones, showed significant cross-correlations between the femoral cortical contents of each of these growth factors and the femoral cortical contents of IGFBP-5 (r=0.625 for IGF-I versus IGFBP-5, r=0.554 for TGF-β versus IGFBP-5, P〈0.001 for each) but not IGFBP-3. Together, these data indicate average net losses of 60% and 29% of the femoral cortical contents of IGF-I and IGFBP-5, respectively, and apparent net losses (i.e., nonsignificant decreases) of 21% and 25% of the femoral cortical contents of IGF-II and TGF-β, respectively, between the third and the sixth decades (i.e., decreases from young adult values of 75.1 pmol/g of bone for IGF-I, 124.7 pmol/g of bone for IGF-II, 0.71 pmol/g of bone for TGF-β, 115.6 pmol/g of bone for IGFBP-5, and 26.2 pmol/g of bone for IGFBP-3).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00310260
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  • 7
    Electronic Resource
    Electronic Resource
    Osteogenic Protein-1 Stimulates mRNA Levels of BMP-6 and Decreases mRNA Levels of BMP-2 and -4 in Human Osteosarcoma Cells (1997)
    Springer
    Calcified tissue international 60 (1997), S. 297 -301 
    Details
    ISSN: 1432-0827
    Keywords: Key words: Bone morphogenetic protein — Osteogenic protein-1 — Transforming growth factor-β— Osteoblasts — Growth factors.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract. Bone morphogenetic proteins (BMPs) are novel growth and differentiation factors that act on mesenchymal stem cells to initiate new bone formation in vivo and promote the growth and differentiation of cells in the osteoblastic lineage. In the present study, we examined the effects of recombinant human osteogenic protein-1 (also known as BMP-7) on the expression of related members of the BMP family using SaOS-2 and U2-OS, two human osteosarcoma cell strains. Evaluation of BMP-2, -4, and -6 mRNA expression indicates that OP-1 stimulated the mRNA levels of BMP-6 in both SaOS-2 cells (threefold) and U2-OS cells (fivefold) after 24 hours of treatment, while decreasing the mRNA levels of BMP-4 in SaOS-2 cells (80%) and BMP-2 and BMP-4 in U2-OS cells by 50% and 72%, respectively. BMP-2 mRNA expression, as examined by Northern blot analysis, was below detectable limits in SaOS-2 cultures. These results demonstrate that OP-1 modulates the mRNA expression of related members of the BMP family, suggesting a possible mode of action of OP-1 on the growth and differentiation of cells in the osteoblastic lineage in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002239900232
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  • 8
    Electronic Resource
    Electronic Resource
    Photoluminescence of as-grown CuAlS2 prepared by iodine transport method (1983)
    Springer
    Il nuovo cimento della Società Italiana di Fisica 2 (1983), S. 1939-1943 
    Details
    ISSN: 0392-6737
    Keywords: Photoluminescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Description / Table of Contents: Riassunto Due nuove bande di luminescenza sono state osservate in cristalli di CuAlS2 come-cresciuti, preparati mediante trasporto di iodio da Al2S3 e Cu2S. La banda con la minore lunghezza d'onda a 77 K appare a circa 2.95 eV, mostrando uno spostamento di spettro con l'intensità di eccitazione, ed è considerata come dovuta all'emissione di coppie donatore-accettore. A temperatura ambiente, l'emissione sembra diventare una transizione da donatore a buca libera. L'emissione con la maggiore lunghezza d'onda appare a 1.8 eV con una grande semiampiezza di 0.5 eV, ha una sua caratteristica banda di eccitazione ed è considerata dovuta ad un centro localizzato.
    Abstract: Резюме В кристаллах CuAlS2, приготовленных методом переноса иода из Al2S2 и Cu2S, наблюдаются две новых люминесцентных полосы. Коротковолновая полоса при 77 К появляерся 2.95 эВ, обнаруживая спектральный сдвиг с интенсивностью возбуждения. Полагается, что эта полоса обусловлена излучением доноракцепторных пар. При комнатной температуре излучение, по-видимому, изменяет переход, «донор-свободная дырка». Длинноволновое излучение появляется при 1.8 эВ с широкой полушириной 0.5 эВ, обладая собственной характеристической полосой возбуждения. Полагается, что это излучение обусловлено локализованным центром.
    Notes: Summary Two new luminescence bands have been observed in as-grown CuAlS2 crystals prepared by iodine transport from Al2S3 and Cu2S. The shorter-wave-length band at 77K appears at around 2.95 eV, showing a spectral shift with excitation intensity, and is considered to be due to a donor-acceptor pair emission. At room temperature, the emission seems to change to a donor-to-free-hole transition. The longer-wave-length emission appears at 1.8 eV with broad half-width of 0.5 eV, having its own characteristic excitation band, and is considered to be due to a localized centre.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02457891
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  • 9
    Electronic Resource
    Electronic Resource
    Photoluminescence of CuGaS2 and heterostructure formation with sputtered ZnS (1983)
    Springer
    Il nuovo cimento della Società Italiana di Fisica 2 (1983), S. 1933-1938 
    Details
    ISSN: 0392-6737
    Keywords: Photoluminescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Description / Table of Contents: Riassunto I cristalli di CuGaS2 cresciuti per trasporto di iodio mostrano fotoluminescenza a temperatura ambiente a 2.45 eV e 1.44 eV. Si mostra che la distribuzione spettrale dell'emissione verde è relativamente ben descritta dalla curva calcolata per una transizione diretta da banda a banda con selezionek. Si è tentata la formazione dell'eterogiunzione tra cristalli di CuGaS2 trattati con zolfo e film amorfi di ZnS a bassa resistività, preparati mediante spruzzamento catodico a temperatura ambiente. La caratteristicaI–V del diodo mostra un comportamento rettificante, ma non si è osservata luminescenza per iniezione.
    Abstract: Резюме Кристаллы CuGaS2 о⇃наруживают при комнатной температуре фатолюминесценцию при 2.45 эВ и 1.44 эВ. Показывается что спектральное распределение зеленого излучения относительно хорошо описывется вычисленной кривой для прямого перехода зоназона сk-отбором. Исследуется образование гетероперехода между кристаллами CuGaS2 и аморфными пленками ZnS с низким сопротивлением, притотовленными распылением при комнатной температуре.I–V характеристика диода обнаруживает выпрямляющее, но инжекционная люминесценция не наблюдается.
    Notes: Summary CuGaS2 crystals grown by iodine transport exhibit room temperature photoluminescences at 2.45 eV and at 1.44 eV. The spectral distribution of the green emission is shown to be relatively well described by the calculated curve for a direct band-to-band transition withk-selection. The heterojunction formation has been tried between sulfur-treated CuGaS2 crystals and low-resistivity amorphous ZnS films prepared by sputtering at room temperature. TheI–V characteristic of the diode shows rectifying behaviour, but no injection luminescence has been observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02457890
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  • 10
    Electronic Resource
    Electronic Resource
    Distribution of cadmium and zinc in tissues and organs, and their age-Related changes in striped dolphins,Stenella coeruleoalba (1983)
    Springer
    Archives of environmental contamination and toxicology 12 (1983), S. 543-550 
    Details
    ISSN: 1432-0703
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Notes: Abstract Cadmium and zinc concentrations were determined in the tissues and organs, including the fetus, of striped dolphins. The kidney showed the highest cadmium concentration and the hepatic cadmium concentration was also relatively high. A similar distribution pattern among soft tissues was also observed with zinc, where its concentration varied less than that of cadmium. A significant positive correlation between zinc and cadmium was found in both the kidney and the liver; the increase of zinc concentration with cadmium was more marked in the liver (Zn:Cd 3∶1 on molar basis) than kidney (Zn:Cd 1∶1). Negligible cadmium concentration in the fetus suggested that cadmium is not transferred to the fetus via the placenta from the mother. Organ-specific age trends of cadmium and zinc concentrations were presented in detail for muscle, liver, kidney, pancreas, brain, and blood. Both metals showed rapid change during the periods of the fetus and weaning.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01056550
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