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  • Artikel  (59)
  • 1995-1999  (37)
  • 1985-1989  (22)
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  • 1
    Digitale Medien
    Digitale Medien
    Target detection and range resolution by the big brown bat (Eptesicus fuscus) using normal and time-reversed model echoes (1989)
    Springer
    Journal of comparative physiology 166 (1989), S. 65-73 
    Details
    ISSN: 1432-1351
    Schlagwort(e): Bats ; Range discrimination ; Echo ; Filtering
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Big brown bats (Eptesicus fuscus) were tested for their ability to detect an electronically simulated target, and to discriminate differences in range to two simulated targets, when receiving either a model of their own sonar emissions or the model reversed in time as the ‘echo’. The theory of matched detection predicts a large decrease in performance if bats use matched filtering, unless they are somehow able to adjust their filter to match the novel, time-reversed signal. The detection thresholds we obtained were much lower than Møhl's (1986), but like him we found no difference in threshold for reversed models (Table 2). This suggests either that bats do not use matched filtering for target detection, or, possibly, that they are able to adapt their filter to a highly unnatural signal in some way as yet unknown. Unlike detection, range discrimination was much poorer with reversed echoes (Table 3). Threshold increased from about 1 cm range difference with normal model echoes to 18 cm or more with reversed model echoes. This suggests that range determination, which is based on measuring the time of arrival of echoes, does involve matched filtering. Whether such filtering is ‘ideal’ (i.e., equivalent to cross-correlation detection) cannot be decided by our results, but there are some indications that the match between an echo and the presumed internal template (the ‘match’ of matched filtering) must be fairly precise. Also, since performance with phantom targets generated using model echoes was as good as has been found with real targets, the internal template is probably fixed (or only slowly modifiable) rather than re-programmed with each sonar emission. Finally, because synchronization of emission and model echo was not perfect, the apparent distance to targets probably varied by 2 to 4 cm from emission to emission, although both targets would appear to move together thus keeping the range difference constant. This suggests that bats determined range to the targets simultaneously rather than sequentially, as is usually assumed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00190211
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  • 2
    facet.materialart.
    Unbekannt
    Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis (1998)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1998-10-23
    Beschreibung: Analysis of the 1,042,519-base pair Chlamydia trachomatis genome revealed unexpected features related to the complex biology of chlamydiae. Although chlamydiae lack many biosynthetic capabilities, they retain functions for performing key steps and interconversions of metabolites obtained from their mammalian host cells. Numerous potential virulence-associated proteins also were characterized. Several eukaryotic chromatin-associated domain proteins were identified, suggesting a eukaryotic-like mechanism for chlamydial nucleoid condensation and decondensation. The phylogenetic mosaic of chlamydial genes, including a large number of genes with phylogenetic origins from eukaryotes, implies a complex evolution for adaptation to obligate intracellular parasitism.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Stephens, R S -- Kalman, S -- Lammel, C -- Fan, J -- Marathe, R -- Aravind, L -- Mitchell, W -- Olinger, L -- Tatusov, R L -- Zhao, Q -- Koonin, E V -- Davis, R W -- AI 39258/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 1998 Oct 23;282(5389):754-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Program in Infectious Diseases, University of California, Berkeley, CA 94720, USA. ctgenome@socrates.berkeley.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9784136" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Aerobiosis ; Amino Acid Sequence ; Amino Acids/biosynthesis ; Bacterial Outer Membrane Proteins/genetics ; Bacterial Proteins/chemistry/genetics ; Biological Evolution ; Chlamydia trachomatis/classification/*genetics/metabolism/physiology ; DNA Repair ; Energy Metabolism ; Enzymes/chemistry/genetics ; *Genome, Bacterial ; Humans ; Lipids/biosynthesis ; Molecular Sequence Data ; Peptidoglycan/biosynthesis/genetics ; Phylogeny ; Protein Biosynthesis ; Recombination, Genetic ; *Sequence Analysis, DNA ; Transcription, Genetic ; Transformation, Bacterial ; Virulence
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    facet.materialart.
    Unbekannt
    DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells (1988)
    American Association for the Advancement of Science (AAAS)
    Details
    Publikationsdatum: 1988-01-15
    Beschreibung: By means of a selective DNA amplification technique called polymerase chain reaction, proviral sequences of the human immunodeficiency virus (HIV-1) were identified directly in DNA isolated from peripheral blood mononuclear cells (PBMCs) of persons seropositive but not in DNA isolated from PBMCs of persons seronegative for the virus. Primer pairs from multiple regions of the HIV-1 genome were used to achieve maximum sensitivity of provirus detection. HIV-1 sequences were detected in 100% of DNA specimens from seropositive, homosexual men from whom the virus was isolated by coculture, but in none of the DNA specimens from a control group of seronegative, virus culture-negative persons. However, HIV-1 sequences were detected in 64% of DNA specimens from seropositive, virus culture-negative homosexual men. This method of DNA amplification made it possible to obtain results within 3 days, whereas virus isolation takes up to 3 to 4 weeks. The method may therefore be used to complement or replace virus isolation as a routine means of determining HIV-1 infection.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ou, C Y -- Kwok, S -- Mitchell, S W -- Mack, D H -- Sninsky, J J -- Krebs, J W -- Feorino, P -- Warfield, D -- Schochetman, G -- New York, N.Y. -- Science. 1988 Jan 15;239(4837):295-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Center for Infectious Diseases, Centers for Disease Control, Atlanta, GA 30333.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3336784" target="_blank"〉PubMed〈/a〉
    Schlagwort(e): Acquired Immunodeficiency Syndrome/*microbiology ; Base Sequence ; DNA, Viral/*blood ; DNA-Directed DNA Polymerase ; *Gene Amplification ; HIV/*genetics/isolation & purification ; HIV Seropositivity ; Homosexuality ; Humans ; Leukocytes, Mononuclear/*analysis ; Male ; Nucleic Acid Amplification Techniques ; Nucleic Acid Hybridization ; Virus Cultivation
    Print ISSN: 0036-8075
    Digitale ISSN: 1095-9203
    Thema: Biologie , Chemie und Pharmazie , Informatik , Medizin , Allgemeine Naturwissenschaft , Physik
    Publiziert von American Association for the Advancement of Science (AAAS)
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
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    Unbekannt
    Quaternary Eustatic Sedimentary Accretion of Southern Bahamas Archpelago: ABSTRACT (1985)
    American Association of Petroleum Geologists
    Details
    Publikationsdatum: 1985-01-01
    Print ISSN: 0149-1423
    Digitale ISSN: 1943-2674
    Thema: Geologie und Paläontologie
    Publiziert von American Association of Petroleum Geologists
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Interaction of gas-phase atomic deuterium with the Ru(001)–p(1×2)–O surface: Kinetics of hydroxyl and water formation (1996)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 104 (1996), S. 7713-7718 
    Details
    ISSN: 1089-7690
    Quelle: AIP Digital Archive
    Thema: Physik , Chemie und Pharmazie
    Notizen: The deuteration of oxygen adatoms on the Ru(001) surface has been investigated by means of temperature programmed desorption and high-resolution electron energy loss spectroscopy. Exposure of gas-phase atomic deuterium to the p(1×2) oxygen overlayer with a fractional adatom coverage of oxygen of 0.5 leads to the production of water at a surface temperature as low as 90 K. After exposure to molecular deuterium, no reaction is observed, suggesting that a direct Eley–Rideal (ER) reaction occurs between the impinging deuterium atoms and the preadsorbed oxygen. Only after a very low exposure of deuterium was it possible to isolate chemisorbed OD groups on the surface, implying that OD formation is the rate-limiting step in the formation of water via ER kinetics on Ru(001). Estimates of the ER reaction cross sections were made, and for the deuteration of adsorbed oxygen and hydroxyls, the cross sections were found to be (7.0±0.3)×10−17 cm2 and (2.2±0.1)×10−15 cm2, respectively. In addition to the ER mechanism, the chemisorbed OD groups could also react with coadsorbed deuterium adatoms via Langmuir–Hinshelwood (LH) kinetics at surface temperatures near 170 K, suggesting an activation barrier that is less than 9 kcal/mol. This implies that OD formation is also the rate-limiting step in the formation of water via LH kinetics on Ru(001). © 1996 American Institute of Physics.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1063/1.471452
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  • 6
    Digitale Medien
    Digitale Medien
    Book reviews (1995)
    Springer
    Formal aspects of computing 7 (1995), S. 581-585 
    Details
    ISSN: 1433-299X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Informatik
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01211634
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    Regulation of mosquitocidal toxin synthesis in Bacillus sphaericus (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 310-314 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract Translational lacZ fusions to the promoters of the parasporal, crystal protein (binary toxin) and 100-kDa ADP-ribosylating mosquitocidal toxin genes of Bacillus sphaericus were prepared and expression of the toxin genes monitored as β-galactosidase activity. Transcription of the crystal protein gene fusion began immediately before the end of exponential growth and continued into stationary phase in both B. sphaericus and Bacillus subtilis but accompanied exponential-phase growth in Escherichia coli. Expression of this fusion was severley delayed in a B. subtilis spo0A mutant and decreased relative to the wild type in a B. subtilis spoIIAC background. β-Galactosidase activity from the 100-kDa toxin gene fusion was restricted to early exponential phase in B. sphaericus, but in B. subtilis it continued into late exponential phase. Expression was about eightfold lower in B. sphaericus than B. subtilis suggesting an element of negative control in the native host.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002530050408
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    Regulation of mosquitocidal toxin synthesis in Bacillus sphaericus (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 310-314 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract Translational lacZ fusions to the promoters of the parasporal, crystal protein (binary toxin) and 100-kDa ADP-ribosylating mosquitocidal toxin genes of Bacillus sphaericus were prepared and expression of the toxin genes monitored as β-galactosidase activity. Transcription of the crystal protein gene fusion began immediately before the end of exponential growth and continued into stationary phase in both B. sphaericus and Bacillus subtilis but accompanied exponential-phase growth in Escherichia coli. Expression of this fusion was severely delayed in a B. subtilis spoOA mutant and decreased relative to the wild type in a B. subtilis spoIIAC background. β-Galactosidase activity from the 100-kDa toxin gene fusion was restricted to early exponential phase in B. sphaericus, but in B.subtilis it continued into late exponential phase. Expression was about eightfold lower in B. sphaericus than b. subtilis suggesting an element of negative control in the native host.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00172830
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  • 9
    Digitale Medien
    Digitale Medien
    Optimization of mosquitocidal toxin synthesis from Bacillus sphaericus using gene fusions (1996)
    Springer
    World journal of microbiology and biotechnology 12 (1996), S. 7-11 
    Details
    ISSN: 1573-0972
    Schlagwort(e): Bacillus sphaericus ; biological control ; fermentation ; gene control
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract β-Galactosidase gene fusions have been used to monitor the progress of mosquito-larvicidal-toxin gene expression in Bacillus sphaericus strain 2362. β-Galactosidase estimation in cells from late-growth-phase batch cultures was compared with larvicidal toxicity after incubation for 48 h. Conditions which promoted efficient sporulation, such as plentiful trace elements and relatively crude protein sources (soybean or cottonseed flours), enhanced reporter gene expression and provided high toxicity. However, acetate, which repressed sporulation, similarly repressed binary toxin yield. Gene fusions to the binary and 100-kDa toxin genes of B. sphaericus could be useful for the rapid screening of fermentation conditions for the local production of this larvicidal bacterium but, in view of the poor correlation with toxicity at high toxicity levels, such experiments should be confirmed with bioassays.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00327790
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  • 10
    Digitale Medien
    Digitale Medien
    Stereoselection in designed three-helix bundle metalloproteins (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Chirality 10 (1998), S. 35-40 
    Details
    ISSN: 0899-0042
    Schlagwort(e): de novo design ; self-assembly ; metalloproteins ; diastereoselection ; Chemistry ; Organic Chemistry
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: The stereochemical consequences of the metal-ion assisted self-assembly of parallel three-helix peptide bundles are investigated. Chiral induction in the self-assembly of systems containing extensive protein secondary structure is compared with the racemic synthesis of short metallopeptides. Isolation and characterization of the individual stereoisomers of an exchange-inert metalloprotein provide structural insights into analogous exchange-labile systems. Chirality 10:35-40, 1998. © 1998 Wiley-Liss,Inc.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/chir.7
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