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  • 1
    Electronic Resource
    Electronic Resource
    Synthesis of an analog of human calcitonin gene related peptide, [Asu2,7]-h-CGRP (1987)
    Springer
    Cellular and molecular life sciences 43 (1987), S. 890-892 
    Details
    ISSN: 1420-9071
    Keywords: h-CGRP analog ; synthesis ; Ca and Pi lowering effects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The analog of h-CGRP, des-Ala-deamino-dicarba-h-CGRP, was synthesized by the combination of the conventional solution and the solid phase peptide synthesis methods. This analog showed stronger and longer-lasting hypocalcemic and hypophosphatemic activities than the natural hormone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01951653
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  • 2
    Electronic Resource
    Electronic Resource
    Anomalous enhancement of DD reaction in Pd and Au/Pd/PdO heterostructure targets under low-energy deuteron bombardment (1998)
    Springer
    JETP letters 68 (1998), S. 823-829 
    Details
    ISSN: 1090-6487
    Keywords: 25.45.−z ; 27.60.+j ; 27.80.+w
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Yields of protons emitted in the D+D reaction in Pd, Au/Pd/PdO, Ti, and Au foils are measured by a d E-E counter telescope for bombarding energies between 2.5 and 10 keV. The experimental yields are compared with those predicted from a parametrization of the cross section and stopping power at higher energies. It is found that for Ti and Au target the enhancement of the D(d,p)T reaction is similar to that observed with a deuterium gas target (several tens of eV). The dependence of the yields on the bombarding energy corresponds well to the screening potential parameters U s=250±15 eV for Pd and 601 ±23 eV for Au/Pd/PdO. Possible models of the enhancement obtained are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1134/1.567800
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  • 3
    Electronic Resource
    Electronic Resource
    A simple and reliable quick-freezing/freeze-fracturing procedure (1997)
    Springer
    Histochemistry and cell biology 107 (1997), S. 81-84 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  We describe a simple method for the quick-freezing/freeze-fracturing of cells in tissues or culture monolayers. Tissue slices or cultured cells were covered with thin copper foil (10-μm-thick), and frozen by smashing them against a liquid helium-cooled copper block. Freeze-fracturing was accomplished by mechanically separating the copper foil from the frozen specimen. The fracture faces were replicated by platinum and carbon. Replicas were processed for conventional electron microscopic observation or cytochemical labeling. This method allows the ultrastructural and cytochemical examination of large areas of fractured membrane without chemical fixation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004180050091
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  • 4
    Electronic Resource
    Electronic Resource
    ‘Males second’ strategy in the allocation of sexes by the parasitic wasp, Gryon japonicum (1989)
    Springer
    Oecologia 81 (1989), S. 145-148 
    Details
    ISSN: 1432-1939
    Keywords: Gryon japonicum ; Parasitoid ; Riptortus clavatus ; Precise sex ratio ; Sex allocation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Patterns of the sex ratio allocation of Gryon japonicum (Ashmead) (Hymenoptera: Scelionidae), a solitary egg parasitoid of Riptortus clavatus (Thunberg) (Heteroptera: Alydidae), were investigated in the laboratory, and the result was checked against the field data on the sex composition of the parasitoid. When five host eggs were presented simultaneously to each of the females of G. japonicum in a laboratory experiment, they had a strong tendency to lay a male egg in second host egg and female eggs in the others. However, when four host eggs were presented to each female more than 3 h after the completion of oviposition on a host egg, most of the females laid male eggs in the third oviposition, i.e. the second host eggs after the experimental interruption of oviposition. These results indicated that there was a mechanism for G. japonicum to produce a male egg in the second host egg in consecutive ovipositions, and that the mechanism was reset by more than 3 h intervals of oviposition. By this mechanism, G. japonicum is thought to produce the precise sex ratio in response to the size of a host egg batch. Field data on the size of a host egg batch and the sex composition of the parasitoid in a host egg batch supported this view.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00379797
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  • 5
    Electronic Resource
    Electronic Resource
    A possible hybrid zone in the Mytilus edulis complex in Japan revealed by PCR markers (1997)
    Springer
    Marine biology 128 (1997), S. 91-95 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The adhesive protein allele of mussels collected at 13 points in Japan from Hokkaido to Kyushu was analyzed by the polymerase chain reaction using a set of primers which amplifies a part of the nonrepetitive region of the adhesive protein gene. While most mussels exhibited a 126 bp fragment, characteristic of the pure Mytilus galloprovincialis, 55 of 64 mussels sampled at Hiura and 1 of 14 mussels at Hakodate Port exhibited 168 and 126 bp fragments. Sequence analysis of the two fragments indicated that the 168 and 126 bp fragments are almost identical to previously reported sequences in M. trossulus and M. galloprovincialis, respectively. Since the frequency of heterozygous individuals in Hiura is very high, it is unlikely that they are simple hybrids. However, it is evident that mixing of genes occurred between the two species off Hokkaido.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002270050072
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  • 6
    Electronic Resource
    Electronic Resource
    A high-speed and continuous 3D measurement system (1995)
    Springer
    Machine vision and applications 8 (1995), S. 79-84 
    Details
    ISSN: 1432-1769
    Keywords: Slit-ray projection ; Position-sensitive device ; 3D range data ; High-speed range finders
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract A new system for high-speed and continuous 3D measurement is presented. It is based on the slit-ray projection method. The key component is the image plane constructed by a position-sensitive device (PSD) array that is horizontally un segmented and linear, but vertically numbered. All PSD row elements are attached to their respective analog signal processors. The analog signals are multiplexed and digitized by multiplexers and A/D converters at appropriate sampling intervals and stored in memory. By virtue of this configuration of the image plane, basis data for 3D measurement are acquired during only one scan of the slit ray at high speed in the form of memory addresses and data. Moreover, if we use large capacity and/or dual port memories, we can continuously obtain 3D range data for as many scenes as needed. The trial system has been implemented with a newly designed 128-channel PSD array and has verified our method, enabling us to obtain successive 3D range data at video rates with an error within±0.3%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01213473
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  • 7
    Electronic Resource
    Electronic Resource
    Life history of the temperate subtidal gastropod Umbonium costatum (1995)
    Springer
    Marine biology 122 (1995), S. 73-78 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The life history and reproductive biology of the trochid sand snail Umbonium costatum (Kiener) were investigated on a subtidal sandy shore in Hakodate Bay, Japan between 1988 and 1991. Female U. costatum mature at 1 yr of age (shell diameter-11 mm), reproduce twice (in June-July and September-October) in successive years, grow to a maximum size (shell diameter=22 mm) at age 8 yr, increase annual fecundity with age from 2000 (age 1 yr) to 37000 (age 8 yr), and show a maximum monthly gonad somatic index of 8% which is constant among ages. In comparison to a previously studied life history of a tropical Umbonium vestiatium, temperate U. costatum shows more sustained growth and a longer life span after maturation. This could be explained by: (1) the optimal size model concerned with resource investment, in gametes (Sebens 1987); and by (2) bet hedging to compensate large variability in larval success at high latitudes. These two hypotheses are not mutually exclusive, but both are based on season-related extremes of environment at high latitudes where the period suitable for reproduction is short.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00349279
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  • 8
    Electronic Resource
    Electronic Resource
    Spatio-temporal population dynamics of the sand snail Umbonium costatum: importance of ontogenetic migration and annual recruitment variability (1995)
    Springer
    Marine biology 123 (1995), S. 815-820 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The spatio-temporal population dynamics of the subtidal snail Umbonium costatum (Kiener) in Hakodate Bay, northern Japan, are described over a 9-yr period (1982 to 1988, 1992). Annual variations in recruitment success not only caused the highly variable age structure of the population, but also affected its distribution pattern. In heavy recruitment years (1982, 1984 and 1988), location of the densest population differed from the other years, and the areas with 0 yr old individuals were larger. Every year, the distribution of 0 yr olds was mostly restricted to inshore (within 320 m from shore), while the distribution of adults (〉0 yr) was mostly restricted to offshore (〉320 m from shore). Such patterns may be generated by ontogenetic migration of cohorts to offshore areas. Ontogenetic migration also could have buffered the large annual variation in spat distribution and maintained the small variation in the distribution of adults. (Prince et al. 1988). Density-related migration has also been reported in Acmaea digitalis (Frank 1965), Patella cochlear (Branch 1974) and Patelloida alticostata (Black 1977). Dense populations of the gastropod Umbonium costatum are found in some clean subtidal sandy-bottoms in Japan, e.g. inner part of Hakodate Bay. In the bay, the age structure of U. costatum revealed predominant year-classes. The 0 yr olds were restricted to the shallowest zone (〈4 m depth) and most adult (〈0 yr) cohorts were restricted to 6 to 7 m depths (Noda 1991a). This paper describes the temporal variability of population structure and distributional patterns of Umbonium costatum, and examines the distribution-regulating processes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00349125
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  • 9
    Electronic Resource
    Electronic Resource
    Geographical distribution of genes for resistance to formae speciales of Erysiphe graminis in common wheat (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 82-88 
    Details
    ISSN: 1432-2242
    Keywords: Erysiphe graminis ; Forma specialis ; Resistance ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The geographical distribution of Pm10, Pm11, Pm14, and Pm15 wheat genes for resistance to inappropriate formae speciales of Erysiphe graminis was investigated using gene-for-gene relationships. Pm10 and Pm15 were very common among many indigenous accessions of common wheat collected from various areas in the world. The diversity of genotypes, which consisted of allelic combination at those loci, was high near the center of origin of common wheat and decreased with increasing distance from the center. In Europe, an apparent contrast of predominant genotypes occurred between the south and the north, suggesting that these genes are useful markers for revealing the routes by which common wheat spread in Europe. On a whole, the genes for resistance to inappropriate formae speciales were observed to be widely distributed throughout the world. We suggest that the difference between these genes and the genes for resistance to races of an appropriate forma specialis may only be in their distribution and that of their corresponding avirulence genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220862
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  • 10
    Electronic Resource
    Electronic Resource
    The characterization of autoallotetraploid hybrids between Iris ensata Thunb. and I. laevigata Fisch. (1998)
    Springer
    Euphytica 103 (1998), S. 325-328 
    Details
    ISSN: 1573-5060
    Keywords: Iris ensata ; I. laevigata ; autoallotetraploid hybrids ; anthocyanins ; crossability ; interspecific cross-breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The characteristics of autoallotetraploid hybrids obtained from the cross between Iris ensata cv. Raspberry Rimmed (4X) and amphidiploids of I. laevigata × I. ensata were examined and compared with those of their parents. The color of inner and outer perianths in the autoallotetraploids were bluish purple and similar to those of the amphidiploid parent. However, the autoallotetraploids exhibited low pollen fertility. In addition, the autoallotetraploids were characterized by 17 or 19 anthocyanins and had high resemblance to their parents in the anthocyanin expression. Among these anthocyanins, malvidin 3RGac5G and petunidin 3RGac5G were regarded as major anthocyanins in the autoallotetraploids and their parents, but the differences in the ratios of malvidin 3RGac5G:petunidin 3RGac5G between the autoallotetraploids and their parents were ca. 2:1 for the former and ca. 1:1 for the latter. No viable hybrid seeds were obtained from the reciprocal crosses between I. ensata (2X and 4X) and the autoallotetraploids. Finally, the interspecific cross-breeding of I. ensata using the autoallotetraploids is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018694817359
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