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  • 1
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    The paucity of grants among young scientists (2002)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2002-12-25
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Campbell, Peter -- New York, N.Y. -- Science. 2002 Dec 20;298(5602):2328-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12498168" target="_blank"〉PubMed〈/a〉
    Keywords: Biological Science Disciplines/*education ; *Education, Graduate ; Great Britain ; Time Factors ; United States
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
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    Cell therapy of alpha-sarcoglycan null dystrophic mice through intra-arterial delivery of mesoangioblasts (2003)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2003-07-12
    Description: Preclinical or clinical trials for muscular dystrophies have met with modest success, mainly because of inefficient delivery of viral vectors or donor cells to dystrophic muscles. We report here that intra-arterial delivery of wild-type mesoangioblasts, a class of vessel-associated stem cells, corrects morphologically and functionally the dystrophic phenotype of virtually all downstream muscles in adult immunocompetent alpha-sarcoglycan (alpha-SG) null mice, a model organism for limb-girdle muscular dystrophy. When mesoangioblasts isolated from juvenile dystrophic mice and transduced with a lentiviral vector expressing alpha-SG were injected into the femoral artery of dystrophic mice, they reconstituted skeletal muscle in a manner similar to that seen in wild-type cells. The success of this protocol was mainly due to widespread distribution of donor stem cells through the capillary network, a distinct advantage of this strategy over previous approaches.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sampaolesi, Maurilio -- Torrente, Yvan -- Innocenzi, Anna -- Tonlorenzi, Rossana -- D'Antona, Giuseppe -- Pellegrino, M Antonietta -- Barresi, Rita -- Bresolin, Nereo -- De Angelis, M Gabriella Cusella -- Campbell, Kevin P -- Bottinelli, Roberto -- Cossu, Giulio -- 1322/Telethon/Italy -- 463/BI/Telethon/Italy -- New York, N.Y. -- Science. 2003 Jul 25;301(5632):487-92. Epub 2003 Jul 10.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Stem Cell Research Institute, H. S. Raffaele, Via Olgettina 58, 20132 Milan, Italy.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12855815" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Blood Vessels/cytology/embryology ; Cell Differentiation ; Cell Line ; Cell Movement ; Cytoskeletal Proteins/*genetics/*metabolism ; Dystrophin/metabolism ; Endothelium, Vascular/physiology ; Female ; Femoral Artery ; Genetic Vectors ; Lentivirus/genetics ; Locomotion ; Male ; Membrane Glycoproteins/*genetics/*metabolism ; Mesoderm/cytology ; Mice ; Mice, Knockout ; Mice, Transgenic ; Muscle Contraction ; Muscle Fibers, Skeletal/cytology/physiology ; Muscle, Skeletal/cytology/metabolism/pathology/*physiology ; Muscular Dystrophy, Animal/metabolism/pathology/*therapy ; Regeneration ; Sarcoglycans ; *Stem Cell Transplantation ; Stem Cells/*physiology ; Transfection
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 3
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    Abnormal coronary function in mice deficient in alpha1H T-type Ca2+ channels (2003)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2003-11-25
    Description: Calcium ion (Ca2+) influx through voltage-gated Ca2+ channels is important for the regulation of vascular tone. Activation of L-type Ca2+ channels initiates muscle contraction; however, the role of T-type Ca2+ channels (T-channels) is not clear. We show that mice deficient in the alpha1H T-type Ca2+ channel (alpha(1)3.2-null) have constitutively constricted coronary arterioles and focal myocardial fibrosis. Coronary arteries isolated from alpha(1)3.2-null arteries showed normal contractile responses, but reduced relaxation in response to acetylcholine and nitroprusside. Furthermore, acute blockade of T-channels with Ni2+ prevented relaxation of wild-type coronary arteries. Thus, Ca2+ influx through alpha1H T-type Ca2+ channels is essential for normal relaxation of coronary arteries.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Chen, Chien-Chang -- Lamping, Kathryn G -- Nuno, Daniel W -- Barresi, Rita -- Prouty, Sally J -- Lavoie, Julie L -- Cribbs, Leanne L -- England, Sarah K -- Sigmund, Curt D -- Weiss, Robert M -- Williamson, Roger A -- Hill, Joseph A -- Campbell, Kevin P -- New York, N.Y. -- Science. 2003 Nov 21;302(5649):1416-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, University of Iowa, Iowa City, IA 52242, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/14631046" target="_blank"〉PubMed〈/a〉
    Keywords: Acetylcholine/pharmacology ; Animals ; Arteries/drug effects/*physiology ; Calcium/*metabolism ; Calcium Channels, T-Type/genetics/*physiology ; Coronary Vessels/drug effects/pathology/*physiology ; Echocardiography ; Electrocardiography ; Endothelium, Vascular/drug effects/physiology ; Female ; Fibrosis ; Ganglia, Spinal/cytology ; Gene Targeting ; Heart/physiology ; Heart Rate ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Muscle, Smooth, Vascular/physiology ; Myocardium/pathology ; Neurons/metabolism ; Nickel/pharmacology ; Nitric Oxide/physiology ; Nitric Oxide Donors/pharmacology ; Nitroprusside/pharmacology ; Patch-Clamp Techniques ; Vasoconstriction/drug effects ; *Vasodilation/drug effects
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 4
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    Statement on scientific publication and security (2003)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2003-02-22
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Atlas, Ronald -- Campbell, Philip -- Cozzarelli, Nicholas R -- Curfman, Greg -- Enquist, Lynn -- Fink, Gerald -- Flanagin, Annette -- Fletcher, Jacqueline -- George, Elizabeth -- Hammes, Gordon -- Heyman, David -- Inglesby, Thomas -- Kaplan, Samuel -- Kennedy, Donald -- Krug, Judith -- Levinson, Rachel -- Marcus, Emilie -- Metzger, Henry -- Morse, Stephen S -- O'Brien, Alison -- Onderdonk, Andrew -- Poste, George -- Renault, Beatrice -- Rich, Robert -- Rosengard, Ariella -- Salzburg, Steven -- Scanlan, Mary -- Shenk, Thomas -- Tabor, Herbert -- Varmus, Harold -- Wimmer, Eckard -- Yamamoto, Keith -- Journal Editors and Authors Group -- New York, N.Y. -- Science. 2003 Feb 21;299(5610):1149.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12595658" target="_blank"〉PubMed〈/a〉
    Keywords: *Access to Information ; *Bioterrorism ; Peer Review, Research ; Periodicals as Topic ; *Publishing ; *Security Measures ; *Terrorism ; United States
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 5
    Electronic Resource
    Electronic Resource
    Single-wall carbon nanotube atomic force microscope probes (2002)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 80 (2002), S. 2002-2004 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We examine the factors that govern the stability of imaging using single-wall C nanotubes as probes for atomic force microscopy. Nonvertical alignment of the nanotubes causes such probes to bend in response to the surface–nanotube interaction forces during imaging. For long nanotubes, this elastic response causes the nanotube tip to jump into contact with the surface and renders it unsuitable for imaging. For short nanotubes, stable noncontact-mode imaging can be achieved using a small cantilever vibration amplitude. However, the bending response is enhanced on highly textured surfaces, which limits the ability to image nonplanar features. © 2002 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1461073
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  • 6
    Electronic Resource
    Electronic Resource
    Ultrathin PtSi layers patterned by scanned probe lithography (2001)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 79 (2001), S. 1109-1111 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: A process for patterning ultrathin layers of PtSi with high spatial resolution is presented. In this process, scanned probe anodic oxidation is used to pattern a surface oxide layer on a H-passivated Si surface. This oxide pattern prevents the reaction of a deposited Pt film with the underlying Si in the formation of PtSi. The unreacted Pt on the oxide is removed by a selective etch before any annealing. This process greatly reduces lateral diffusion and produces a 2-nm-thick PtSi layer with good electrical properties that maintains the fidelity of the patterned oxide mask. Such nanostructured PtSi films are a good candidate for use in constructing lateral Si-based quantum devices. © 2001 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1394167
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  • 7
    Electronic Resource
    Electronic Resource
    The kinetics and mechanism of scanned probe oxidation of Si (2000)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 76 (2000), S. 1782-1784 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We report measurements of the kinetics of scanned probe oxidation under conditions of high humidity and pulsed bias. For a hydrophobic Si surface the oxidation rate for short pulse times (∼10 ms) is controlled by the density of H2O molecules in the ambient humidity surrounding the tip-sample interface. At longer pulse times (∼0.1 s) liquid H2O bridges this interface and the maximum oxidation rate increases by a factor of ∼104 because of the increased density of H2O molecules. We propose that the rate-limiting step of the oxidation process is the production of O anions from the ambient humidity. © 2000 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.126166
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  • 8
    Electronic Resource
    Electronic Resource
    Phylogeography and intraspecific genetic variation of prochilodontid fishes endemic to rivers of northern South America (2004)
    Oxford, UK; Malden , USA : Blackwell Publishing Ltd/Inc
    Journal of fish biology 64 (2004), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Phylogeography and intraspecific genetic variation were studied in prochilodontids endemic to the Orinoco, Essequibo and Amazon River basins of northern South America. Portions of two protein-encoding mitochondrial (mt) DNA genes, ND4 and COI, were examined using single-strand conformational polymorphisms (SSCPs) and nucleotide sequencing. Phylogeographic analysis indicated that the geographically widespread Prochilodus rubrotaeniatus is paraphyletic, with individuals from the Orinoco sharing most recent common ancestry with co-occurring Prochilodus mariae. A second Prochilodus rubrotaeniatus clade was composed of haplotypes found in the Rio Cuyuní(Essequibo Basin) and tributaries of the Rio Negro (Amazon Basin). Intraspecific genetic analysis suggested that a complex set of processes have influenced patterns of genetic variation in prochilodontid lineages. Prochilodus rubrotaeniatus is monomorphic at both loci in the Rio Negro and probably recently colonized this basin from the Rio Essequibo. Only two of 55 P. mariae exhibited variant haplotypes, and both had resulted from non-synonymous changes in the ND4 region. These observations were counter to neutral expectation and consistent with the action of natural selection on the mitochondrion. Overall, these analyses implicate vicariance, demography and selection for driving diversification of prochilodontids in northern South America.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.2004.00299.x
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  • 9
    Electronic Resource
    Electronic Resource
    Cataract preventative role of mammalian blood meal, histidine, iron and zinc in diets for Atlantic salmon (Salmo salar L.) of different strains (2003)
    Oxford, UK : Blackwell Science Ltd
    Aquaculture nutrition 9 (2003), S. 0 
    Details
    ISSN: 1365-2095
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: In the period from winter 1996 to autumn 1999, three feeding trials were conducted to study the possible role of mammalian blood meal in preventing lens opacities in Atlantic salmon. In addition to blood meal supplemented feed, experimental diets were formulated, aiming to mimic the total concentrations of selected elements present in blood meal (BM) enriched diets; the amino acid histidine (His) and the mineral iron (Fe). In addition, these diets were supplemented with Zn. The trials included different rearing conditions, sizes and strains of Atlantic salmon; one small scale feeding experiment with pre-smolt of Strain A in fresh water (Trial 1) and two semi large trials with sub-yearling (S0s) and yearling (S1s) smolts of Strains A and B in seawater (Trials 2 and 3). The trials included duplicate or triplicate feeding groups and lasted 6–12 months. Standard commercial diets not supplemented with BM were used as controls. High prevalence of cataract developed over time in all three studies, with end point ranges as follows; Trial 1: 40–52 %; Trial 2: 51–83 %; Trial 3: 63–100%. The cataracts normally first appeared in the anterior and/or posterior cortical region and later affected the perinuclear region. In all the experiments, there was a significant cataract preventative effect of both the BM supplemented and experimental diets compared to the respective control diets. To some extent, cataract incidence was reduced, but primarily cataractogenesis was less severe in these groups. A concurrent positive effect on growth of both added BM and experimental diets was observed in the two seawater trials, probably related to maintaining vision and feed uptake ability. In one of the trials, there were significant differences in cataract susceptibility between the two strains, measured both as frequency and severity of cataractous changes. Our data suggest that elevated concentrations of dietary His and/or Fe mitigate cataract formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2095.2003.00269.x
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  • 10
    Electronic Resource
    Electronic Resource
    Effect of Frictional Heating on the Serum Lubricant and Wear of UHMWPE Cups against Cobalt-Chrome or Zirconia Balls (2001)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 218-220 (Nov. 2001), p. 525-530 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/46/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.218-220.525.pdf
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