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  • oxylipins  (2)
  • Cytidine Deaminase  (1)
  • Keywords: Saccharomyces cerevisiae; aerobic; chemostat; growth kinetics  (1)
  • Transfection  (1)
  • 2000-2004  (4)
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Keywords
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Anomalies in the growth kinetics of Saccharomyces cerevisiae strains in aerobic chemostat cultures (2000)
    Springer
    Journal of industrial microbiology and biotechnology 24 (2000), S. 231-236 
    Details
    ISSN: 1476-5535
    Keywords: Keywords: Saccharomyces cerevisiae; aerobic; chemostat; growth kinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Aerobic glucose-limited chemostat cultivations were conducted with Saccharomyces cerevisiae strains NRRL Y132, ATCC 4126 and CBS 8066, using a complex medium. At low dilution rates all three strains utilised glucose oxidatively with high biomass yield coefficients, no ethanol production and very low steady-state residual glucose concentrations in the culture. Above a threshold dilution rate, respiro-fermentative (oxido-reductive) metabolism commenced, with simultaneous respiration and fermentation occurring, which is typical of Crabtree-positive yeasts. However, at high dilution rates the three strains responded differently. At high dilution rates S. cerevisiae CBS 8066 produced 7–8 g ethanol L−1 from 20 g glucose L−1 with concomitant low levels of residual glucose, which increased markedly only close to the wash-out dilution rate. By contrast, in the respiro-fermentative region both S. cerevisiae ATCC 4126 and NRRL Y132 produced much lower levels of ethanol (3–4 g L−1) than S. cerevisiae CBS 8066, concomitant with very high residual sugar concentrations, which was a significant deviation from Monod kinetics and appeared to be associated either with high growth rates or with a fermentative (or respiro-fermentative) metabolism. Supplementation of the cultures with inorganic or organic nutrients failed to improve ethanol production or glucose assimilation. Journal of Industrial Microbiology & Biotechnology (2000) 24, 231–236.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/sj.jim.2900813
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A novel oxylipin-associated ‘ghosting’ phenomenon in yeast flocculation (2000)
    Springer
    Antonie van Leeuwenhoek 77 (2000), S. 401-406 
    Details
    ISSN: 1572-9699
    Keywords: flocculation ; ghosting ; lectin ; oxylipins ; yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Research on the distribution of oxylipins (3-hydroxy fatty acids) in flocculant strains of the yeast Saccharomyces cerevisiae led to the uncovering of a novel ‘ghosting’ phenomenon observed during assumed lectin-mediated aggregation. We found that intracellular oxylipin-containing osmiophilic layers migrate through yeast cell walls in a ‘ghostlike’ fashion without visually affecting the cell wall structure or the layers. This migration resulted in the binding of these layers to cell walls of adjacent cells. Consequently, ‘ghosting’ seems a prerequisite for flocculation to occur. However, ‘ghosting’ alone may not be sufficient to ensure flocculation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002735216303
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  • 3
    Electronic Resource
    Electronic Resource
    Ascospore aggregation and oxylipin distribution in the yeast Dipodascopsis tothii (2000)
    Springer
    Antonie van Leeuwenhoek 77 (2000), S. 389-392 
    Details
    ISSN: 1572-9699
    Keywords: ascospore ultrastructure ; assembly ; Dipodascopsis tothii ; oxylipins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Upon cultivation of the yeast Dipodascopsis tothii in its sexual stage, small ascospores are released individually from the ascus tip, which then assemble in sheathed cluster balls. In contrast to Dipodascopsis uninucleata, this yeast produced smooth bean shaped ascospores with sheath-like appendages that assemble in a disordered sheathed ball of ascospores outside the ascus. Strikingly, upon release, the ascus tip contained 3-hydroxy oxylipins, while the released ascospore clusters contained little or no 3-hydroxy oxylipins as indicated by immunofluorescence microscopy. In D. uninucleata, these oxylipins are concentrated on the spore surface and interspore matrix, but not on the ascus tip.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002789025872
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  • 4
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    Comment on "Inhibition of hepatitis B virus replication by APOBEC3G" (2004)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2004-09-09
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rosler, Christine -- Kock, Josef -- Malim, Michael H -- Blum, Hubert E -- von Weizsacker, Fritz -- New York, N.Y. -- Science. 2004 Sep 3;305(5689):1403; author reply 1403.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Medicine II, University of Freiburg, Hugstetter Strasse 55, D-79106 Freiburg, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15353783" target="_blank"〉PubMed〈/a〉
    Keywords: Cell Line, Tumor ; Cytidine Deaminase ; DNA, Viral/genetics/metabolism ; Genome, Viral ; Hepatitis B virus/genetics/*physiology ; Humans ; Nucleoside Deaminases ; *Point Mutation ; Proteins/*metabolism ; RNA, Viral/*metabolism ; Repressor Proteins ; Transfection ; Virus Replication
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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