Publication Date:
2003-11-08
Description:
Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid-based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence was developed to refine this draft map to a higher confidence map of 4679 proteins and 4780 interactions. Statistical modeling of the network showed two levels of organization: a short-range organization, presumably corresponding to multiprotein complexes, and a more global organization, presumably corresponding to intercomplex connections. The network recapitulated known pathways, extended pathways, and uncovered previously unknown pathway components. This map serves as a starting point for a systems biology modeling of multicellular organisms, including humans.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Giot, L -- Bader, J S -- Brouwer, C -- Chaudhuri, A -- Kuang, B -- Li, Y -- Hao, Y L -- Ooi, C E -- Godwin, B -- Vitols, E -- Vijayadamodar, G -- Pochart, P -- Machineni, H -- Welsh, M -- Kong, Y -- Zerhusen, B -- Malcolm, R -- Varrone, Z -- Collis, A -- Minto, M -- Burgess, S -- McDaniel, L -- Stimpson, E -- Spriggs, F -- Williams, J -- Neurath, K -- Ioime, N -- Agee, M -- Voss, E -- Furtak, K -- Renzulli, R -- Aanensen, N -- Carrolla, S -- Bickelhaupt, E -- Lazovatsky, Y -- DaSilva, A -- Zhong, J -- Stanyon, C A -- Finley, R L Jr -- White, K P -- Braverman, M -- Jarvie, T -- Gold, S -- Leach, M -- Knight, J -- Shimkets, R A -- McKenna, M P -- Chant, J -- Rothberg, J M -- HG01536/HG/NHGRI NIH HHS/ -- New York, N.Y. -- Science. 2003 Dec 5;302(5651):1727-36. Epub 2003 Nov 6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉CuraGen Corporation, 555 Long Wharf Drive, New Haven, CT 06511, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/14605208" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Calcium/metabolism
;
Cell Cycle
;
Cell Differentiation
;
Cloning, Molecular
;
Computational Biology
;
DNA, Complementary
;
Drosophila Proteins/*metabolism
;
Drosophila melanogaster/*genetics/*metabolism/physiology
;
Genes, Insect
;
Immunity, Innate
;
Mathematics
;
Models, Statistical
;
Photoreceptor Cells, Invertebrate/cytology
;
Protein Binding
;
*Protein Interaction Mapping
;
*Proteome
;
RNA Splicing
;
RNA, Messenger/genetics/metabolism
;
Receptor, Epidermal Growth Factor/metabolism
;
Signal Transduction
;
Transcription, Genetic
;
Two-Hybrid System Techniques
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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