ALBERT

Notes: An experiment was carried out with Cichlasoma urophthalmus (Günther) juveniles to determine the phosphorus requirement and its interaction with dietary calcium. Twelve isoenergetic and isoproteic diets were prepared using a basal artificial diet containing vitamin-free casein, dextrin, starch, corn oil, fish oil, vitamin mixture and a mineral mixture free of calcium and phosphorus. Calcium and phosphorus levels were determined in the casein. To the basal diets were added different concentrations of phosphorus as potassium monophosphate (0.5, 1.0, 1.5 and 2.5 g kg–1) and calcium as calcium carbonate (0.5, 0.75, 1.0, 1.5, 2.0, 2.5, 3.0 and 4.0 g kg–1). These concentrations resulted in varying Ca–P ratios (1:1, 1.33:1, 1.5:1, 1.6:1 and 2.0:1). Calcium and phosphorus concentrations in the water were 84 mg kg–1 and 0.003 mg kg–1, respectively. The diet with 0.5 g kg–1 phosphorus resulted in deficiency signs such as reduced growth, high conversion ratio, high fat content and low bone mineralization. Increased levels of dietary calcium and phosphorus both gave improved growth and mineralization. Mineralization continued to increase with dietary phosphorus levels above that required for maximum growth. The optimum level of phosphorus in the diet was 1.5 g kg–1, the optimum calcium level was 1.8 g kg–1 and the optimum Ca–P ratio was 1.3. Carcass lipid levels were inversely related to dietary phosphorus.

Notes: The effect of the proportion of Calluna vulgaris cover on diet composition, intake and performance of sheep grazing hill vegetation communities in northern Spain is examined. A total of 591 non-lactating Gallega ewes grazed for five consecutive grazing seasons (June to September) on replicated plots of hill pastures (1700 m.a.s.l.) composed principally of Festuca, Agrostis, Nardus and Calluna spp. but with different proportions of Calluna vulgaris cover, either 0·3 (C0·3) or 0·7 (C0·7) of the total area. In 1 year, twenty-eight ewes suckling single lambs also grazed the plots. The mean stocking density over the 5 years was 8·7 ewes ha–1. On treatment C0·3, daily liveweight gains (33 g d–1) of non-lactating ewes were significantly (P 〈 0·001) greater than on treatment C0·7 (12 g d–1). Likewise in lactating ewes the difference in mean daily liveweight change was 40 g d–1 (–5 vs. –45 g d–1 for C0·3 and C0·7 treatments respectively; P 〈 0·001). Liveweight gains of lambs were only 80–100 g d–1 from June to August and lambs only maintained live weight during August and September. The effect of lactational status on liveweight changes was not significant. Liveweight gains of non-lactating ewes increased significantly (P 〈 0·001) from the first to the last year of the experiment on both treatments.The composition of the diet was significantly affected by treatment (P 〈 0·001), with a higher proportion of grass species on the C0·3 treatment and a higher digestibility of the diet in the first half of the grazing season (P 〈 0·001). The proportion of C. vulgaris in the diet was significantly (P 〈 0·001) higher on the C0·7 treatment and increased significantly (P 〈 0·001) from July to September on both treatments. There were no significant differences in the composition of the diet selected by lactating and non-lactating ewes.The results demonstrate that on hill vegetation communities, in which the grass components (Festuca rubra, Agrostis capillaris, and Nardus stricta) cover at least 0·3 of the area and on which the preferred grass component (Festuca and Agrostis spp.) is maintained at a sward height of at least 2·5 cm, non-lactating ewes can increase their live weight and body condition, but this increase is influenced by the proportion and quantity of species of grass in the diet, which is affected in turn by the species of grass available and their nutritive quality. However, ewes suckling lambs were not able to maintain their live weight and body condition except when Calluna cover was 0·3 and grass height was more than 3·5 cm.It is concluded that these indigenous vegetation communities can be used in sheep production systems to complement the use of improved pastures at other times of year. In particular, they can be utilized during the non-lactating period (summer) to increase body condition before the beginning of the mating period in autumn.

Notes: Nitrogen metabolism in Aspergillus nidulans is regulated by AREA, a member of the GATA family of transcription factors. One mechanism that modulates AREA activity involves the rapid degradation of the areA transcript when sufficient NH4+ or Gln are available. This signalling mechanism has been shown to require a region of 218 nucleotides within the 3′ untranslated region of areA mRNA. We demonstrate that this region functions independently in a heterologous transcript and acts to accelerate degradation of the poly(A) tail, which in turn leads to rapid transcript degradation in response to the addition of NH4+ or Gln to the growth medium. areA transcript degradation is inhibited by cycloheximide, but this is not a general consequence of translational inhibition. We believe that this is the first reported example in which specific physiological signals, acting through a defined sequence within a transcript, have been shown to promote accelerated poly(A) degradation, which in turn triggers transcript degradation.

Notes: Saccharomyces cerevisiae PAU genes constitute the largest multigene family in yeast, with 23 members located mainly in subtelomeric regions. The role and regulation of these genes were previously unknown. We detected PAU gene expression during alcoholic fermentation. An analysis of PAU gene regulation using PAU–lacZ fusions and Northern analyses revealed that they were regulated by anaerobiosis. PAU genes display, however, different abilities to be induced by anaerobiosis and this appears to be related to their chromosomal localization; two subtelomeric copies are more weakly inducible than an interstitial one. We show that PAU genes are negatively regulated by oxygen and repressed by haem. Examination of PAU gene expression in rox1Δ and tup1Δ strains indicates that PAU repression by oxygen is mediated by an unknown, haem-dependent pathway, which does not involve the Rox1p anaerobic repressor but requires Tup1p. Given the size of the gene family, PAU genes could be expected to be important during yeast life and some of them probably help the yeast to cope with anaerobiosis.

Notes: 1. A year-round study was conducted in a mesotrophic reservoir to determine the dynamics of zooplankton populations as a function of food availability (edible phytoplankton), nutrient concentration, temperature and hydraulic regime.2. Rotifer biomass was correlated with soluble reactive phosphorus (SRP) concentration. The abundance of the rotifers Keratella cochlearis and Anuraeopsis fissa were not correlated with food availability (measured by chlorophyll and cell counts) but showed a strong dependence on P availability. Another rotifer, Synchaeta oblonga, and crustacean species were not related to nutrient availability but seemed to be dependent on food concentrations, especially of some phytoplankton taxa.3. In this field study, rotifers seemed more susceptible than Daphnia or copepods to P-limitation. Among rotifer species, Keratella seemed to be more susceptible than Anuraeopsis to P limitation. Different susceptibilities of zooplankton species to nutrient limitation may be important in explaining the dynamics of these organisms in natural situations. Further analyses are warranted to clarify the interactions between nutrient limitation and energy limitation among zooplankton.

Notes: 1. Reproduction between Atlantic salmon males and interspecific hybrid Salmo salar × Salmo trutta females was monitored in a controlled flow channel diverted from a south European river located at the edge of Atlantic salmon natural geographic distribution in Europe.2. Post-F1 hybrids were viable and survived in the wild, at least until dispersal from redds. After transfer to hatchery conditions, 67% survived into the second year.3. The hybrids possessed 98 chromosomes: two sets of Atlantic salmon(2n = 58) and one set of brown trout (n = 40) chromosomes.4. The existence of a low proportion of allotriploid individuals can be expected in rivers where Atlantic salmon and brown trout populations coexist.

Notes: The construction cost of fine roots was studied in 23 woody species and two grassland communities, growing under natural conditions in southern Spain. Calculation of the energy (glucose) required for their synthesis was based on the quantification of chemical components present in tissues. Despite considerable differences in the chemical composition of the three life forms studied (trees, shrubs and herbaceous), detected differences in construction cost were non-significant (mean value: 1·64 ± 0·13 g glucose g−1). However, shrubs and herbaceous plants growing in more fertile habitats expended significantly less energy on root synthesis (1·58 ± 0·06 and 1·41 ± 0·05 g glucose g−1, respectively) than those growing in less fertile areas (1·80 ± 0·06 and 1·57 ± 0·1 g glucose g−1, respectively), because they contained smaller amounts of either waxes (shrubs) or lignins (herbaceous), both expensive to synthesize, and, proportionately, more cellulose; which is inexpensive to synthesize. Deciduous and evergreen tree species also differed mainly with regard to wax and cellulose contents, giving rise to a significantly higher construction cost in evergreens (1·57 ± 0·07 g glucose g−1 versus 1·78 ± 0·02 g glucose g−1). The differences observed in construction cost appeared to be due more to habitat-induced differences in chemical composition than to any intrinsic difference between the species studied.

Notes: Root construction and maintenance costs were estimated in four evergreen and three deciduous Quercus species that are typical in the landscape of southern Spain. The cost quantification was based on analysis of the growth–­respiration ratio. Values observed for both construction cost (ranging from 1·17 to 1·29 g glucose g−1 dry weight) and maintenance   cost   (ranging   from   6·22   to   11·71 mg glucose g−1 dry weight d−1) were generally lower than those reported in other studies. The results showed non-significant differences between deciduous and evergreen species. The lack of significant differences between species appeared to be due to the homogeneity of growth conditions. Hydroponic culture, with unrestricted nutrient and water supply, would lead to low tissue carbon content and low respiration rates, leading to the low costs observed. Furthermore, the fact that root organs are clearly importers of organic molecules inevitably entails some underestimation of the respiration associated with growth and, to a lesser extent, with maintenance respiration. This leads in turn to underestimation of the corresponding construction and maintenance costs. All this raises doubts as to the suitability of this method for studying root systems.

Notes: 1. Genetic variation at five microsatellite loci was analysed in a collection of scales (1970–97) sampled from Atlantic salmon adults returning to the Nivelle River (South France).2. Native and foreign fish (from an allochthonous Scottish stock introduced into the river to increase population size) were clearly identified as all foreign individuals released in the river were physically marked.3. Introgression of foreign genes into the native gene pool has occurred, although the reproductive success of foreign Atlantic salmon in the wild was lower than that of native individuals.4. The utility of old scale samples for investigating the impact of foreign stocking on wild fish populations is demonstrated in this work.

Notes: Field resistance of Echinochloa spp. to propanil has been previously reported in Costa Rica, Colombia and Arkansas (USA). In this study, the mechanism of resistance was investigated in three resistant (R) and three susceptible (S) biotypes. The shoot fresh weight reduction in pot-grown plants from a post-emergence spray of propanil at 2.44 kg a.i. ha−1 on biotypes R/S from Costa Rica, Colombia and Arkansas was 35/98%, 25/79% and 20/82% respectively. In vitro chlorophyll fluorescence data from leaf tissue incubated in propanil showed that photosynthesis was inhibited in all biotypes, indicating that the propanil-binding site and enzyme were not altered. After transfer to herbicide-free solution, photosynthesis recovered only in resistant biotypes, indicating that the mechanism of resistance was caused by enhanced metabolism of the herbicide. Simultaneous treatment with fenitrothion, an aryl acylamidase inhibitor, prevented the recovery of photosynthesis in leaf tissue in two resistant biotypes. In contrast, the cytochrome P450 mono-oxygenase inhibitor, 1-aminobenzotriazole, did not prevent recovery from propanil in leaf tissue. Application of 14C-propanil to the second leaf of intact Echinochloa plants showed that c. 90% of the radioactivity remained in the treated leaf for up to 72 h after application. No major differences in translocation between R and S biotype plants were found. TLC analysis of tissue extracts from the treated leaves showed substantially less radioactivity associated with propanil, present after 72 h in rice or in the three R biotypes, compared with S biotypes.