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  • Springer  (18)
  • 2000-2004  (8)
  • 1965-1969  (10)
  • 1925-1929
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  • 1
    Electronic Resource
    Electronic Resource
    Effect of ethacrynic acid and furosemide on the transport systems of the rat intestine (1968)
    Springer
    Naturwissenschaften 55 (1968), S. 232-233 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00606224
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  • 2
    Electronic Resource
    Electronic Resource
    A photoisomerisation product of dieldrin (1966)
    Springer
    Bulletin of environmental contamination and toxicology 1 (1966), S. 127-132 
    Details
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01844243
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  • 3
    Electronic Resource
    Electronic Resource
    The accumulation of amino acids by various preparations of dog intestine (1965)
    Springer
    Cellular and molecular life sciences 21 (1965), S. 12-13 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Résumé On a séparé le tissu intestinal en muqueuse et muscle, lesquels ont été incubés in vitro dans une solution d'acide aminé marqué; ainsi on a pu étudier l'absorption intracellulaire de l'acide aminé. On a trouvé que la musculature intestinale ne peut accumuler un acide aminé contre un gradient de concentration. Il semble qu'il existe dans le muscle une barrière contre la diffusion dont la significance est discutée.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02136353
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  • 4
    Electronic Resource
    Electronic Resource
    Rapid purification of transfected porcine muscle cells (2000)
    Springer
    Methods in cell science 22 (2000), S. 217-223 
    Details
    ISSN: 1573-0603
    Keywords: Flow cytometry ; Muscle cell ; Porcine ; Transfection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A detailed methodology is described for fluorescence-activated cell sorting (FACS) of porcine muscle cells that have been transfected to express green fluorescent protein (GFP). Cells are liberated from porcine skeletal muscle and primary cultures are transfected with DNA encoding GFP. Primary cultures are subjected to immunocytochemistry using a primary muscle-specific monoclonal antibody followed by incubation with a phycoerythrin-conjugated second antibody. Transfected myoblasts aresorted from fibroblasts using forward angle light scatter and ninety degree light scatter, phycoerythrin fluorescence, and GFP fluorescence. These procedures allow for isolation of genetically-engineered porcine muscle cells more rapidly than traditional clonal selection procedures. Consequently, FACS provides porcine myoblast populations that retain the majority of their replicative capacity and are not contaminated with non-myogenic cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009856411749
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  • 5
    Electronic Resource
    Electronic Resource
    Application of BARE-1 retrotransposon markers to the mapping of a major resistance gene for net blotch in barley (2000)
    Springer
    Molecular genetics and genomics 264 (2000), S. 325-334 
    Details
    ISSN: 1617-4623
    Keywords: BARE-1 retrotransposon Barley Net blotch resistance Linkage mapping Quantitative trait locus (QTL)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Net blotch, which is caused by the fungus Pyrenophora teres Drechs. f. teres Smedeg., presents a serious problem for barley production worldwide, and the identification and deployment of sources of resistance to it are key objectives for many breeders. Here, we report the identification of a major resistance gene, accounting for 65% of the response variation, in a cross between the resistant line CI9819 and the susceptible cv. Rolfi. The resistance gene was mapped to chromosome 6H with the aid of two recently developed systems of retrotransposon-based molecular markers, REMAP and IRAP. A total of 239 BARE-1 and Sukkula retrotransposon markers were mapped in the cross, and the 30-cM segment containing the locus with significant resistance effect contained 26 of the markers. The type and local density of the markers should facilitate future map-based cloning of the resistance gene as well as manipulation of the resistance through backcross breeding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380000326
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  • 6
    Electronic Resource
    Electronic Resource
    The preservation unaltered, of mineral nitrogen in tropical soils and soil extracts (1967)
    Springer
    Plant and soil 27 (1967), S. 53-80 
    Details
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Experiments have been conducted on different methods of storing soils and soil extracts to preserve the mineral-N status unaltered. Neither the addition of chemicals at the rates used nor storage at sub-zero temperatures were found to preserve the mineral-N status of the soils completely unaltered over short periods of time. Furthermore, evidence was found to show that storage at sub-zero temperatures can effect subsequent mineralization of nitrogen during incubation. The mineral-N content of soil extracts prepared with an acidified K2SO4 reagent was not preserved over short periods by the addition of chemicals, by storing the extracts at sub-zero temperatures or by the addition of copper ions to the extracting solution. The mineral-N content of soil extracts prepared with a neutral KCl reagent could be preserved during storage at subzero temperatures but the addition of copper ions to the extracting reagent was not effective. Changes in the mineral-N content of both soils and soil extracts during storage were not consistent and in the soils appeared to be related to biological activity and the concentration of mineral N present. In soil extracts changes appeared to be related to the concentration of mineral-N present and to some other factor(s).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01373978
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  • 7
    Electronic Resource
    Electronic Resource
    A comparison of autoclaved and gamma-irradiated soils as media for microbial colonization experiments (1967)
    Springer
    Plant and soil 27 (1967), S. 239-248 
    Details
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effects on various properties of Lincoln Clay of a sterilizing dose of gamma radiation (3.0 megarads) were compared to the effects resulting from autoclaving the soil (121°C) for 1 hour. Effects of both treatments were much more drastic when moist, rather than air-dry soil was treated but, in general, radiation had less effect on soluble organic matter and on total water-extractable electrolyte than did autoclaving. Radiation caused a greater release of NH4-N from soil treated moist than did autoclaving but the reverse was true in dry soil. Alcohol-soluble ninhydrin-positive material was increased by both sterilization procedures with irradiation having the greater effect. The aggregate stability of Lincoln clay was decreased by irradiation and increased by autoclaving. Pure cultures of bothArthrobacter sp. andPseudomonas sp. grew better, on the basis of cell yields, in irradiated than in autoclaved soil. Respiration of mixed soil organisms in an artificial soil amended with an extract of irradiated soil was almost identical with that in an extract of air-dried soil but auto-claved soil extract was only metabolized after a prolonged lag period.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01373392
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  • 8
    Electronic Resource
    Electronic Resource
    Stable resistance in barley to Pyrenophora teres f. teres isolates from the Nordic-Baltic region after increase on standard host genotypes (2000)
    Springer
    Euphytica 113 (2000), S. 71-77 
    Details
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; net blotch ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Results from tests of a mixture of Finnish net blotch, Pyrenophora teres Drechs. f. teres Smedeg., isolates on a differential series of barley seedlings, comprising 17genotypes, indicated that patterns of infection response (IR)and percentage leaf area damaged (PLAD) were unaffected by differences in seedling size. Variation of the concentration of inoculum between 1,250 conidia ml-1 and 20,000 conidia ml-1 produced similar patterns of IR and PLAD on the differential series. IR and PLAD scored on the second seedling leaf differentiated resistance to P. teres f. teresamong the genotypes better than on the first seedling leaf. Ina second experiment, 120 single-spore P. teres f. teres isolates from Finland, Sweden, Norway, Latvia, Estonia and Ireland were used in tests conducted in the greenhouse to differentiate them in terms of virulence reaction on seedlings of six differential barley genotypes. Each isolate was tested directly following isolation from the leaf material and after having passaged each through barley cvs. Arve or Pohto, to produce 360 isolates in total. Virulence of the isolates differed significantly on the members of the differential series, but differences associated with country of origin and passaging, and interactions, were small. It is concluded that little variation between virulence of P. teres f. teres isolates is evident over a large geographic area, incorporating Nordic and Baltic countries, and Ireland. Barley genotype response to P. teres f. teres appeared to be of more significance than relative virulence of the pathogen isolates. This could simplify breeding barley for improved resistance to this phytopathogen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003912825455
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  • 9
    Electronic Resource
    Electronic Resource
    Ozone tolerance and antioxidant enzyme activity in soybean cultivars (2000)
    Springer
    Photosynthesis research 64 (2000), S. 15-26 
    Details
    ISSN: 1573-5079
    Keywords: ascorbate peroxidase ; glutathione reductase ; oxidative stress ; ozone tolerance ; superoxide dismutase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The current study confirmed earlier conclusions regarding differential ozone (O3) tolerances of two soybean cultivars, Essex and Forrest, and evaluated antioxidant enzyme activities of these two varieties based on their performance under environmentally relevant, elevated O3 conditions. The experiment was conducted in open-top chambers in the field during the 1994 and 1995 growing seasons. Exposure of plants to moderately high O3 levels (62.9 nl l−1 air, 2-year seasonal average) caused chlorophyll loss and increased membrane permeability when compared to control plants grown in charcoal filtered air (24.2 nl l−1 air). The other effects of O3 treatment were decrease in seed yield, loss of total sulfhydryl groups, reduction of soluble protein content, and increase in guaiacol peroxidase activity in leaves of both cultivars. The O3-induced increase in guaiacol peroxidase activity was much smaller in cv. Essex leaflets. Cv. Essex had less leaf oxidative damage and smaller reduction in seed yield than cv. Forrest under elevated O3 conditions. During ozonation, mature leaflets of the more O3 tolerant cv. Essex had higher levels of glutathione reductase (30%), ascorbate peroxidase (13%), and superoxide dismutase (45%) activity than did mature leaflets of cv. Forrest. Cu,Zn-superoxide dismutase, which represented 95% of total superoxide dismutase activity in the two cultivars, appeared to be increased by O3 exposure in the leaflets of O3 tolerant cv. Essex but not in those of cv. Forrest. Cytosolic ascorbate peroxidase activity was also higher in leaflets of cv. Essex than in cv. Forrest regardless of O3 level. Stromal ascorbate peroxidase and Mn-superoxide dismutase activity did not appear to be involved in the O3 tolerance of the two soybean cultivars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026500911237
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  • 10
    Electronic Resource
    Electronic Resource
    Tolerance of a field grown soybean cultivar to elevated ozone level is concurrent with higher leaflet ascorbic acid level, higher ascorbate-dehydroascorbate redox status, and long term photosynthetic productivity (2000)
    Springer
    Photosynthesis research 64 (2000), S. 77-87 
    Details
    ISSN: 1573-5079
    Keywords: ascorbic acid ; ascorbate-glutathione cycle ; bean yield ; dehydroascorbate ; ozone ; photosynthesis ; soybean ; vegetative yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We examined the characteristics of ascorbic acid (ASC) level, dehydroascorbate (DHA) level, and the ASC–DHA redox status in the leaflets of two soybean cultivars grown in a field environment and exposed to elevated ozone (O3) levels. These two cultivars, one that preliminary evidence indicated to be O3-tolerant (cv Essex), and one that was indicated to be O3-sensitive (cv Forrest), were grown in open-top chambers during the summer of 1997. The plants were exposed daily to a controlled, moderately high O3 level (≈58 nl l−1 air) in the light, beginning at the seedling stage and continuing to bean maturity. Concurrently, control plants were exposed to carbon-filtered, ambient air containing a relatively low O3 level (≈24 nl l−1 air) during the same period. Elevated O3 did not affect biomass per plant, mature leaf area accretion, or bean yield per plant of cv Essex. In contrast, elevated O3 level decreased the biomass and bean yield per plant of cv Forrest by approximately 20%. Daily leaflet photosynthesis rate and stomatal conductance per unit area did not decrease in either cultivar as a result of prolonged O3 exposure. A 10% lower mature leaflet area in O3-treated cv Forrest plants contributed to an ultimate limitation in long-term photosynthetic productivity (vegetative and bean yield). Possible factors causing cv Essex to be more O3 tolerant than cv Forrest were: 1) mature leaflets of control and O3-treated cv Essex plants consistently maintained a higher daily ASC level than leaflets of cv Forrest plants, and 2) mature leaflets of cv Essex plants maintained a higher daily ASC–DHA redox status than leaflets of cv Forrest plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026508227189
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