ALBERT — All Library Books, journals and Electronic Records Telegrafenberg

1

Electronic Resource

The effect of temperature and pH on the growth efficiency of the thermoacidophilic bacterium Bacillus acidocaldarius in continuous culture (1983)

Farrand, Stephen G. ; Jones, Colin W. ; Linton, John D. ; [et al.]

Springer

Archives of microbiology 135 (1983), S. 276-283

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ISSN: 1432-072X

Keywords: Molar growth yields ; Thermoacidophile ; Respiratory chain energy conservation ; Membrane permeability ; Temperature and pH ; Bacillus acidocaldarius

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The molar yield coefficients (Y glucose, Y O 2) of glucose-limited continuous cultures of the thermoacidophile Bacillus acidocaldarius have been measured as a function of dilution rate as well as over a range of temperature and pH (51°C to 64°C, pH 2.8–5.5) at a fixed dilution rate of approximately 0.1 h-1. The highest growth yields were observed at 51°C and pH〉4.3 (Y glucose 54.8 g cells · mol glucose-1, Y O 2 15.0 g cells · mol O 2 -1 ), but were very much lower than those of mesophilic neutrophiles of similar respiratory chain composition to B. acidocaldarius. Even lower growth yields were observed when the temperature was raised or when the pH was lowered, lowest yields occurring at 64°C and pH 2.8 (Y glucose 23.4 g cells · mol glucose-1, Y O 2 5.9 g cells · mol O 2 -1 ). These decreases in growth yield could be correlated with increases in the permeability of the cytoplasmic membrane to protons, i.e. cells needed to catalyse enhanced rates of substrate oxidation in order to avoid a potentially lethal acidification of the cytoplasm. This strategy appears to be successful in that the specific death rates in situ were very low for all cultures except those growing under the most extreme conditions (64°C, pH 2.8).

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00413481

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2

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The use of response surface analysis to study the growth of Bacillus acidocaldarius throughout the growth range of temperature and pH (1983)

Farrand, Stephen G. ; Linton, John D. ; Stephenson, Richard J. ; [et al.]

Springer

Archives of microbiology 135 (1983), S. 272-275

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ISSN: 1432-072X

Keywords: Bacillus acidocaldarius ; Response surface analysis ; Temperature and pH

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract A minimal-salts medium has been optimised to support the growth of the acidophilic thermophilic bacterium Bacillus acidocaldarius. This medium was used during a study of the effect of temperature and pH on the growth rate and growth yield of this organism in batch cultures; a statistical method was used to design the experimental points, and the data were subjected to a response surface analysis which allowed the growth rate and growth yield to be predicted over the entire temperature and pH range from a minimum number of experimental points. The results indicate different responses for growth rate (optimum, 60°C, pH 4.1) and growth yield (optimum tending towards low temperature and neutral pH).

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00413480

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3

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Monoclonal antibodies to feline sarcoma virus gag and fes gene translational products (1983)

Veronese, Fulvia ; Kelloff, Gary J. ; Reynolds, Fred H. ; [et al.]

New York, N.Y. : Wiley-Blackwell

Journal of Cellular Biochemistry 21 (1983), S. 9-18

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ISSN: 0730-2312

Keywords: feline sarcoma virus proteins ; v-fes ; v-fms ; tyrosine ; monoclonal antibodies ; tyrosine phosphorylation ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Chemistry and Pharmacology , Medicine

Notes: A series of hybridomas have been isolated which produce monoclonal antibodies directed against polyprotein gene products of the Gardner, Snyder-Theilen, and McDonough strains of FeSV. Within these are representatives of several immunoglobulin classes including IgG1, IgG2a, IgG2b, IgG2c, and IgM. Antibody produced by one hybridoma recognizes immunologic determinants localized within an FeLV gag gene structural component (p15) common to polyproteins encoded by all three FeSV isolates whereas antibody produced by a second is specific for p30 determinants unique to P170gag-fms. Additional hybridomas secrete antibody directed against v-fes-encoded determinants common to the Gardner and Snyder-Theilen FeSV-encoded polyproteins. GA P110gag-fes and ST P85gag-fes immunoprecipitated by antibody directed against p15 exhibit tyrosine-specific protein kinase activity but lack such activity when precipitated by antibody specific for their acquired sequence (v-fes) components.

Additional Material: 5 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/jcb.240210103

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4

Electronic Resource

The human c-abl oncogene in the philadelphia translocation (1984)

Groffen, John ; Stephenson, John R. ; Heisterkamp, Nora ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Journal of Cellular Physiology 121 (1984), S. 179-191

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ISSN: 0021-9541

Keywords: Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Additional Material: 8 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/jcp.1041210421

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5

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Morphological and ultrastructural characterization of mammalian spermatozoa processed for flow cytometric DNA analyses (1984)

Garner, D. L. ; Johnson, L. A. ; Lake, S. ; [et al.]

New York, NY : Wiley-Blackwell

Gamete Research 10 (1984), S. 339-351

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ISSN: 0148-7280

Keywords: spermatozoa ; flow cytometry ; DNA staining ; nuclear morphology ; ultrastructure ; mammals ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology

Notes: The morphological and ultrastructural changes that occur during preparation of porcine, bovine, and murine spermatozoa for flow cytometric quantification of the relative DNA content of the X- and Y-chromosome-bearing sperm populations were examined. Ejaculated spermatozoa from the boar and bull were washed using a series of dimethyl sulfoxide (DMSO) solutions prior to fixation, whereas the epididymal mouse spermatozoa were washed only in phosphate-buffered saline (PBS). Spermatozoa from all three species were then fixed in ethanol and processed for fluorochrome staining by a treatment regimen consisting of sulfhydryl reduction and proteolysis. The processed sperm nuclei were stained for DNA with the fluorochrome, 4′-6-diamidino-2-phenylindole (DAPI) before quantification by flow cytometry. Scanning and transmission electron micrographs of sperm heads taken at various steps of the preparation and staining procedures show 1) that the rigorous washing procedure disrupted the plasma and outer acrosomal membranes, 2) that ethanol fixation resulted in removal of the outer membranes and disintegration of the nuclear envelope, and 3) that thiol and proteolysis treatment removed the remaining cellular organelles including the tail and rapidly induced partial decondensation of the tightly packed chromatin. Sequential micrographs showed that the nuclear matrix of all three species increased in thickness about twofold during the preparation and staining. Consequently, the harsh procedures currently used for quantitative staining of DNA for high-resolution flow cytometric analyses destroy most cellular organelles and thereby prevent simultaneous characterization of DNA content and other sperm cell constituents.

Additional Material: 6 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/mrd.1120100402

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Crustal-scale pop-up structure in cratonic lithosphere: DOBRE deep seismic reflection study of the Donbas fold belt, Ukraine (2003)

Maystrenko, Y. ; Stovba, S. ; Stephenson, R. ; [et al.]

In: Geology

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Publication Date: 2020-02-12

Keywords: 550 - Earth sciences

Type: info:eu-repo/semantics/article

Format: application/pdf

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The formation of the south-eastern part of the Dniepr–Donets Basin: 2-D forward and reverse modelling taking into account post-rift redeposition of syn-rift salt (2003)

Stovba, S. ; Maystrenko, Y. ; Stephenson, R. ; [et al.]

In: Sedimentary Geology

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Publication Date: 2020-02-12

Description: Forward and reverse modelling of structure and stratigraphy has been used to investigate the syn-rift (Late Devonian) and early post-rift (Carboniferous) evolution of the south-eastern part of the Dniepr–Donets Basin (DDB). Modelling was carried out with and without taking into consideration the withdrawal and surface extrusion of Devonian salt during the formation of salt diapirs. The great thickness of Carboniferous deposits can be explained by the superimposed actions of three processes: post-rift thermal subsidence, withdrawal of Devonian salt from the mother layer during phases of salt diapir activity, and regional subsidence of the East European Platform. The effects of other tectonic and/or non-tectonic processes are not required. Forward syn-rift modelling using the flexural cantilever model of sedimentary basin formation predicts the total syn-rift extension across the southeastern DDB to be approximately 65 km with a maximum stretching factor of 2.4. Shallowing of the Moho during the syn-rift phase is estimated to be 15 km. The present-day Moho, after thermal subsidence and basin fill, is predicted to be 4–6 km shallower than surrounding regions. In the axial zone of the south-eastern DDB the thickness of the Devonian syn-rift sequence may have reached 7.5 km by the end of the rift stage. This is 3–3.5 km more than at present. The thicknesses reduction is due to the outflow of Devonian salt during post-rift periods of halokinetic activity in the early Visean, the middle Serpukhovian, and in the Early Permian. The withdrawal of salt from the mother layer produced additional accommodation space and up to 1.5–1.7 km of the total eventual thickness of the Carboniferous sedimentary succession can be explained as a result of this.

Keywords: 550 - Earth sciences

Type: info:eu-repo/semantics/article

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On the origin of the Southern Permian Basin, Central Europe (2000)

van Wees, J. ; Stephenson, R. ; Ziegler, P. ; [et al.]

In: Marine and Petroleum Geology

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Publication Date: 2020-02-12

Description: A detailed study of the structural and stratigraphic evolution of the Southern Permian Basin during latest Carboniferous to Early Jurassic times, supported by quantitative subsidence analyses and forward basin modelling for 25 wells, leads us to modify the conventional model for the Rotliegend-Zechstein development of this basin. The Late Permian-Early Jurassic tectonic subsidence curves are typical for a Permian to Early Triassic extensional stage that is followed by thermal subsidence. However, a purely extensional model is extremely problematic because active faulting during this time is ''minor' and generally hard to document. Using inverse techniques to model the subsidence curves, we quantitatively show that a significant component of Late Permian and Triassic tectonic subsidence can be explained by thermal relaxation of Early Permian lithospheric thinning, and by delayed infilling of paleo-topographic depressions that developed during the Early Permian. In this interpretation, Stephanian-Autunian wrenching resulted in thermal destabilisation of the lithosphere, deep fracturing of the crust, disruption and erosion of its sedimentary cover and regional uplift of the area of the future Southern Permian Basin. Upon termination of wrench tectonics and associated volcanism, towards the end of the Autunian, the Southern Permian Basin began to subside in response to thermal contraction of the lithosphere. The evolving basin was isolated from the World oceans and had subsided possibly up to some 700 m below their level at the beginning of Upper Rotliegend sedimentation. After catastrophic flooding of this paleo-topographic depression at the beginning of the Zechstein, changing sea level, sedimentation and subsidence rates remained essentially in balance. Although the effects of Triassic rifting overprinted parts of the Southern Permian Basin, its overall subsidence pattern persisted well into the Jurassic. In contrast to the remainder of the Southern Permian Basin, Permian and Triassic crustal extension contributed significantly to the subsidence of the Polish Trough.

Keywords: 550 - Earth sciences

Type: info:eu-repo/semantics/article

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