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  • Analytical Chemistry and Spectroscopy  (2)
  • Hordeum vulgare  (1)
  • Porcine
  • barley
  • bean yield
  • 2000-2004  (3)
  • 1985-1989  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Stable resistance in barley to Pyrenophora teres f. teres isolates from the Nordic-Baltic region after increase on standard host genotypes (2000)
    Springer
    Euphytica 113 (2000), S. 71-77 
    Details
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; net blotch ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Results from tests of a mixture of Finnish net blotch, Pyrenophora teres Drechs. f. teres Smedeg., isolates on a differential series of barley seedlings, comprising 17genotypes, indicated that patterns of infection response (IR)and percentage leaf area damaged (PLAD) were unaffected by differences in seedling size. Variation of the concentration of inoculum between 1,250 conidia ml-1 and 20,000 conidia ml-1 produced similar patterns of IR and PLAD on the differential series. IR and PLAD scored on the second seedling leaf differentiated resistance to P. teres f. teresamong the genotypes better than on the first seedling leaf. Ina second experiment, 120 single-spore P. teres f. teres isolates from Finland, Sweden, Norway, Latvia, Estonia and Ireland were used in tests conducted in the greenhouse to differentiate them in terms of virulence reaction on seedlings of six differential barley genotypes. Each isolate was tested directly following isolation from the leaf material and after having passaged each through barley cvs. Arve or Pohto, to produce 360 isolates in total. Virulence of the isolates differed significantly on the members of the differential series, but differences associated with country of origin and passaging, and interactions, were small. It is concluded that little variation between virulence of P. teres f. teres isolates is evident over a large geographic area, incorporating Nordic and Baltic countries, and Ireland. Barley genotype response to P. teres f. teres appeared to be of more significance than relative virulence of the pathogen isolates. This could simplify breeding barley for improved resistance to this phytopathogen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003912825455
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  • 2
    Electronic Resource
    Electronic Resource
    Tolerance of a field grown soybean cultivar to elevated ozone level is concurrent with higher leaflet ascorbic acid level, higher ascorbate-dehydroascorbate redox status, and long term photosynthetic productivity (2000)
    Springer
    Photosynthesis research 64 (2000), S. 77-87 
    Details
    ISSN: 1573-5079
    Keywords: ascorbic acid ; ascorbate-glutathione cycle ; bean yield ; dehydroascorbate ; ozone ; photosynthesis ; soybean ; vegetative yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We examined the characteristics of ascorbic acid (ASC) level, dehydroascorbate (DHA) level, and the ASC–DHA redox status in the leaflets of two soybean cultivars grown in a field environment and exposed to elevated ozone (O3) levels. These two cultivars, one that preliminary evidence indicated to be O3-tolerant (cv Essex), and one that was indicated to be O3-sensitive (cv Forrest), were grown in open-top chambers during the summer of 1997. The plants were exposed daily to a controlled, moderately high O3 level (≈58 nl l−1 air) in the light, beginning at the seedling stage and continuing to bean maturity. Concurrently, control plants were exposed to carbon-filtered, ambient air containing a relatively low O3 level (≈24 nl l−1 air) during the same period. Elevated O3 did not affect biomass per plant, mature leaf area accretion, or bean yield per plant of cv Essex. In contrast, elevated O3 level decreased the biomass and bean yield per plant of cv Forrest by approximately 20%. Daily leaflet photosynthesis rate and stomatal conductance per unit area did not decrease in either cultivar as a result of prolonged O3 exposure. A 10% lower mature leaflet area in O3-treated cv Forrest plants contributed to an ultimate limitation in long-term photosynthetic productivity (vegetative and bean yield). Possible factors causing cv Essex to be more O3 tolerant than cv Forrest were: 1) mature leaflets of control and O3-treated cv Essex plants consistently maintained a higher daily ASC level than leaflets of cv Forrest plants, and 2) mature leaflets of cv Essex plants maintained a higher daily ASC–DHA redox status than leaflets of cv Forrest plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026508227189
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  • 3
    Electronic Resource
    Electronic Resource
    Rapid purification of transfected porcine muscle cells (2000)
    Springer
    Methods in cell science 22 (2000), S. 217-223 
    Details
    ISSN: 1573-0603
    Keywords: Flow cytometry ; Muscle cell ; Porcine ; Transfection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A detailed methodology is described for fluorescence-activated cell sorting (FACS) of porcine muscle cells that have been transfected to express green fluorescent protein (GFP). Cells are liberated from porcine skeletal muscle and primary cultures are transfected with DNA encoding GFP. Primary cultures are subjected to immunocytochemistry using a primary muscle-specific monoclonal antibody followed by incubation with a phycoerythrin-conjugated second antibody. Transfected myoblasts aresorted from fibroblasts using forward angle light scatter and ninety degree light scatter, phycoerythrin fluorescence, and GFP fluorescence. These procedures allow for isolation of genetically-engineered porcine muscle cells more rapidly than traditional clonal selection procedures. Consequently, FACS provides porcine myoblast populations that retain the majority of their replicative capacity and are not contaminated with non-myogenic cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009856411749
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  • 4
    Electronic Resource
    Electronic Resource
    Identification of some abnormal haemoglobins by fast atom bombardment mass spectrometry and fast atom bombardment tandem mass spectrometry (1988)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 16 (1988), S. 41-44 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The characterization of two abnormal human haemoglobins by fast atom bombardment (FAB) mapping is presented. The first variant, called ‘R’, exhibits a tryptic FAB map identical to that of normal haemoglobin. However, using Staphylococcus protease V8, a peptide containing the carboxyl end of the β-chain exhibits a mass shift down to 300 mass units. This clearly indicates the deletion of the two last amino acids of the β-chain. The second variant, called ‘Grenoble’, is due to two different modifications of the β-chain. The location of the Pro → Ser exchange on peptide T5 is achieved by the collisionally activated dissociation mass analyzed ion kinetic energy spectra of the corresponding [MH]+ ion. The m/z value of that peptide indicated a supplementary acid → amide modification, which was located by amino acid sequencing using chemical methods. This work concludes with the necessity of using complementary methods for achieving rapid determinations of abnormal proteins with minute amounts.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200160108
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  • 5
    Electronic Resource
    Electronic Resource
    The reactions of the ions [XC]+, [X2C]+·, [X3C]+· and [X4C]+· (where X = H or F) with ethene and propene (1987)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 22 (1987), S. 154-156 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The reaction of C1 ions with ethene and propene has been studied in the gas phase using a triple quadrupole mass spectrometer.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210220307
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