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  • Isozymes  (25)
  • Springer  (25)
  • American Association for the Advancement of Science
  • 2000-2004  (2)
  • 1985-1989  (23)
  • 1925-1929
Collection
Keywords
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  • Springer  (25)
  • American Association for the Advancement of Science
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Genetic diversity in Cucumis sativus L. assessed by variation at 18 allozyme coding loci (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 119-128 
    Details
    ISSN: 1432-2242
    Keywords: Biochemical loci ; Cucumber ; Isozymes ; Multivariate analysis ; Starch-gel electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The genetic diversity of the U.S. Cucumis sativus L. germplasm collection [757 plant introductions (PI) representing 45 countries] was assessed using 40 enzymes which represented 74 biochemical loci. Polymorphisms were observed at 18 loci (G2dh-1, Gpi-1, Gpi-2, Gr-1, Gr-2, Idh, Mdh-1, Mdh-2, Mdh-3, Mpi-2, Pepla-2, Peppap-2, Per-4, Pgd-1, Pgd-2, Pgm-1, Pgm-3, and Skdh). Two PIs (285606 and 215589) contained alleles [G2dh-1(1) and Per-4(2), respectively] which did not occur in any other PI. Other alleles which occurred in low frequencies (in 〈 1% of the PIs) included Gpi-1(3), Gpi-2(3), Gr-1(3), Gr-2(1), Idh(1), Mdh-1(2), Mdh-2(1), Peppap-2(1), and Pgd-1(1). Individual loci containing more than one allele in greater than 20% of the PIs included Mpi-2, Pepla-2, Pgd-2, and Pgm-1. Multivariate analyses aided in the reduction of data (principle components), depicted relationships among PIs (cluster), and identified the most discriminating enzyme loci (Pgm-1, Pepla-2, Gr-1, Pgd-2, Mpi-2, and Skdh) (classification and regression tree).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00299764
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  • 2
    Electronic Resource
    Electronic Resource
    RFLP-based genetic maps of wheat homoeologous group 7 chromosomes (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 495-504 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Genetic mapping ; RFLPs ; Isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction fragment length polymorphism (RFLP) mapping was attempted using 18 cDNA clones, 14 anonymous and 4 of known function, which had been shown to have homologous DNA sequences on the group 7 chromosomes of wheat. The loci identified by these probes have been mapped on one or more chromosomes in this homoeologous group using linkage data derived from various F2, random inbred, doubled haploid and single chromosome recombinant populations. The maps also include three isozyme loci, five disease resistance loci, two anthocyanin pigment loci and a vernalisation response locus. The mapping data have been used to determine the extent of map co-linearity over the A, B and D genomes, the degree of RFLP variability in the three genomes and the relative efficiency of various restriction enzymes in detecting RFLPs in wheat. The strategy for future mapping in wheat, particularly the use of “alien” genomes or segments, such as that from Aegilops ventricosa used here, is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00290833
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  • 3
    Electronic Resource
    Electronic Resource
    Genetic variation and migration pathways of maritime pine (Pinus pinaster Ait) in the Iberian peninsula (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 89-95 
    Details
    ISSN: 1432-2242
    Keywords: Key words Pinus pinaster ; Genetic variability ; Isozymes ; Migration pathways
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The genetic variability and migration pathways of Pinus pinaster after glaciation in the Iberian peninsula was studied by means of 18 loci from 12 natural populations of the species. The analysis showed the existence of three groups of populations with different levels of diversity and patterns of recolonization. The southern Iberian group displays a high level of diversity, with a stepping-stone model of variation. The presence of rare alleles in this group and their position in the phylogenetic tree suggest the existence of refugia during glaciations in this zone. The eastern Iberian group also has high levels of diversity but is clearly separate from the first group based on their genetic distances. The Atlantic group displays a low level of diversity that could be interpreted as a rapid recolonization of the entire area by the Eastern group that has not yet developed to a divergence in this area. The southern Iberian range is indicated to be the dispersal centre of the species after the last glaciation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050013
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  • 4
    Electronic Resource
    Electronic Resource
    The impact of domestication on distribution of allozyme variation within and among cultivars of radish,Raphanus sativus L. (1985)
    Springer
    Theoretical and applied genetics 69 (1985), S. 393-398 
    Details
    ISSN: 1432-2242
    Keywords: Breeding methods ; Isozymes ; Polymorphism ; Radish ; Raphanus sativus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Allozyme surveys of cultivated plant species generally report little within-cultivar variation, but considerable among-cultivar variation. This trend contrasts with natural plant populations in which most allozyme variation resides within, rather than among, populations. The difference may be an artifact of the extreme inbreeding techniques used to develop and propagate these crops, rather than a consequence of domestication per se. To test this hypothesis, we compared the population genetic structure of 24 lines of radish cultivars — a domesticated species developed and maintained as open-pollinated, outcrossed populations — with four wild radish populations in California. Although the wild populations displayed more overall allozyme variation than the cultivars, most of the allozyme variation in the cultivars remains partitioned within, rather than among, lines. Apparently, how a crop is developed and maintained can have a profound influence on the organization of genetic variation of that species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00570908
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  • 5
    Electronic Resource
    Electronic Resource
    Allelic variation at α-Amylase loci in hexaploid wheat (1985)
    Springer
    Theoretical and applied genetics 70 (1985), S. 400-406 
    Details
    ISSN: 1432-2242
    Keywords: Hexaploid wheat ; Endosperm ; Allelic variation ; α-Amylase ; Isozymes ; Isoelectric focusing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A study of α-amylase isozyme patterns from gibberellin-induced endosperms from more than 200 wheat genotypes has revealed allelic variation at five of the six α-Amy-1 and α-Amy-2 structural loci. These differences will find application as genetic markers and in varietal identification. The α-Amy-B1 locus on chromosome 6B was most variable and displayed eight distinct allelic forms. The nature of the allelic phenotypes, observations of segregating populations and the number of in vivo translation products of mRNAs from the α-Amy-1 and α-Amy-2 loci indicated that the individual loci are multigenic, each consisting of tightly linked subunits which produce several different isoforms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273745
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  • 6
    Electronic Resource
    Electronic Resource
    Phylogeny of Cucumis based on isozyme variability and its comparison with plastome phylogeny (1985)
    Springer
    Theoretical and applied genetics 71 (1985), S. 430-436 
    Details
    ISSN: 1432-2242
    Keywords: Isozymes ; Phylogeny ; Cucumis ; Chloroplast DNA ; Dendrogram
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An electrophoretic comparison of 29 nuclear-coded enzymes was carried out for 21 Cucumis species, and a phylogeny based on pairwise measurements of the respective genetic distances was computed. This phylogeny was compared to the one based on chlDNA cariation (Perl-Treves and Galun 1985). The two phylogenies were found to share the main dendrogram features; they also agree well with most taxonomic data available on Cucumis. Accordingly, most of the African Cucumis species form a close group (“Anguria group” — “Group A”), which is distant from the melon (C. melo), and from a few other distinct species, all of which are far apart from each other. The cucumber (C. sativus) is the most distant species within the genus. Some specific taxonomic implications as well as some general evolutionary problems related to such a parallel investigation of the nuclear genome and the plastome are evaluated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00251183
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  • 7
    Electronic Resource
    Electronic Resource
    Biochemical genetic basis of downy mildew resistance in pearl millet (1986)
    Springer
    Theoretical and applied genetics 71 (1986), S. 648-651 
    Details
    ISSN: 1432-2242
    Keywords: Sclerospora graminicola ; Peroxidase ; Polyphenoloxidase ; Isozymes ; Pennisetum typhoides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The study of phenolic content and activities of peroxidase and polyphenoloxidase in relation to the degree of downy mildew infection of 12 pearl millet cultivars revealed that these were linearly related to the degree of resistance at both the 30 and 50 day growth stages. Useful electrophoretic differences in peroxidase and polyphenoloxidase were also observed with respect to the expression of resistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264270
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  • 8
    Electronic Resource
    Electronic Resource
    Duplicated chromosome segments in maize (Zea mays L.): further evidence from hexokinase isozymes (1986)
    Springer
    Theoretical and applied genetics 72 (1986), S. 178-185 
    Details
    ISSN: 1432-2242
    Keywords: Hexokinase (HEX) ; Zea mays L. ; Isozymes ; Gene duplication ; Genetic linkage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The genetic control of hexokinase isozymes (ATP: d-hexose-6-phosphotransferase, E.C. 2.7.7.1, HEX) in maize (Zea mays L.) was studied by starch gel electrophoresis. Genetic analysis of a large number of inbred lines and crosses indicates that the major isozymes observed are encoded by two nuclear loci, designated Hex1 and Hex2. Five active allozymes and one null variant are associated with Hex1, while Hex2 has nine active alleles in addition to a null variant. Alleles at both loci govern the presence of single bands, with no intragenic or intergenic heteromers visible, suggesting that maize HEX's are active as monomers. Organelle preparations demonstrate that the products of both loci are cytosolic. All alleles, including the nulls, segregate normally in crosses. Vigorous and fertile plants were synthesized that were homozygous for null alleles at both loci, suggesting that other hexosephosphorylating enzymes exist in maize that are undetected with our assay conditions. Linkage analyses and crosses with B-A translocation stocks place Hex1 on the short arm of chromosome 3, 27 centimorgans from Pgd2 (phosphogluconate dehydrogenase) and Hex2 on the long arm of chromosome 6, approximately 45 centimorgans from Pgd1. It is suggested that the parallel linkages among these two pairs of duplicated genes reflects an evolutionary history involving chromosome segment duplication or polyploidy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266990
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  • 9
    Electronic Resource
    Electronic Resource
    Genetic control of dipeptidase in the Triticeae (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 387-390 
    Details
    ISSN: 1432-2242
    Keywords: Isozymes ; Dipeptidase ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Isoelectric focusing has been employed to elucidate the genetic control of a series of dipeptidase isozymes in wheat and its relatives. The phenotype of wheat shows four bands, three of which are shown by aneuploid analysis to be controlled by the loci Dip-A1, Dip-B1 and Dip-D1 on chromosome arms 6AL, 6BL and 6DL, respectively. Varietal polymorphism for Dip-A1 and Dip-B1 was observed. Different homoeoloci were found in barley, Haynaldia villosa and Agropyron junceum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00274722
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  • 10
    Electronic Resource
    Electronic Resource
    Genetic mapping of an ancient translocation in the genus Lens (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 883-892 
    Details
    ISSN: 1432-2242
    Keywords: Lens ; Isozymes ; Translocation break-point ; Linkage ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Segregation of 18 marker genes was monitored in selfed progeny of a Lens culinaris × L. ervoides hybrid; five linkage groups were mapped, one of which contained a reciprocal translocation break-point that differentiates between the parents. Four markers were found to be linked to the translocation break-point: Aco-1 and Pgm-2 on one side and Gs and Got-2 on the other. The gene pairs on both sides of the translocation are not linked in L. culinaris or in L. orientalis. The L. ervoides gene order was also found in L. odemensis but with significantly reduced map distances. Analysis of monogenic segregations in a number of Lens inter-specific crosses revealed some consistent patterns of deviations from the expected Mendelian ratios. The factors responsible for these unequal segregations, genotypic effects on recombination frequencies, negative interference, and the possible ancient origin of the translocation are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00289394
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