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  • Animal manure  (1)
  • Muscle cell  (1)
  • Russian wheat aphid  (1)
  • Springer  (3)
  • 2000-2004  (1)
  • 1990-1994  (2)
  • 1965-1969
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Nutrient cycling in agroecosystems 40 (1994), S. 165-173 
    ISSN: 1573-0867
    Keywords: Animal manure ; eutrophication ; ground water ; nitrogen ; phosphorus ; surface runoff
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract With the rapid growth of the poultry industry in Oklahoma, U.S.A., more litter is applied to farm land. Thus, information is required on the impact of applications on regional soil and water resources. The effect of soil and poultry litter management on nitrogen (N) and phosphorus (P) loss in runoff and subsurface flow from four 16 m2 plots (Ruston fine sandy loam, 6 to 8% slope) was investigated under natural rainfall. Plots under Bermudagrass (Cynodon dactylon) received 11 Mg litter ha−1, which amounts to contributions of approximately 410 kg N and 140 kg P ha−1 yr−1. In spring, litter was broadcast on 3 of the plots; the upper half of one and total area of the other two. One of the total-area broadcast plots was tilled to 6 cm, the other remained as no till. The fourth plot served as a control. Relative to the control, litter application increased mean concentrations of total N and total P in runoff during the 16-week study for no-till (15.4 and 5.8 mg L−1) and tilled treatments (16.7 and 6.1 mg L−1). However, values for the half-area application (5.6 and 2.0 mg L−1) were similar to the control (5.7 and 1.3 mg L−1). Interflow (subsurface lateral flow at 70 cm depth) P was not affected by litter application; however, nitrate-N concentrations increased from 0.6 (control) to 2.9 mg L−1 (no till). In all cases, 〈 2 % litter N and P was lost in runoff and interflow, maintaining acceptable water quality concentrations. Although litter increased grass yield (8518 kg ha−1) compared to the control (3501 kg ha−1), yields were not affected by litter management. An 8-fold increase in the plant available P content of surface soil indicates long-term litter management and application rates will be critical to the environmentally sound use of this nutrient resource.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 62 (1992), S. 213-217 
    ISSN: 1573-5060
    Keywords: Russian wheat aphid ; Diuraphis noxia ; barley ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The inheritance of resistance to Russian wheat aphid Diuraphis noxia (Mordvilko), in two resistant barleys, Hordeum vulgare L., ASE/2CM//B76BB and ‘Gloria/Come’, was studied in the field and in the greenhouse. The resistant genotypes were crossed with susceptible genotypes ‘Esperanza’ and ‘Shyri’. Resistance reactions of F1, BC1, and F2 plants, and individual F2 plant derived F3 families indicated that resistance in each genotype was controlled by the same single dominant gene.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Methods in cell science 22 (2000), S. 217-223 
    ISSN: 1573-0603
    Keywords: Flow cytometry ; Muscle cell ; Porcine ; Transfection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A detailed methodology is described for fluorescence-activated cell sorting (FACS) of porcine muscle cells that have been transfected to express green fluorescent protein (GFP). Cells are liberated from porcine skeletal muscle and primary cultures are transfected with DNA encoding GFP. Primary cultures are subjected to immunocytochemistry using a primary muscle-specific monoclonal antibody followed by incubation with a phycoerythrin-conjugated second antibody. Transfected myoblasts aresorted from fibroblasts using forward angle light scatter and ninety degree light scatter, phycoerythrin fluorescence, and GFP fluorescence. These procedures allow for isolation of genetically-engineered porcine muscle cells more rapidly than traditional clonal selection procedures. Consequently, FACS provides porcine myoblast populations that retain the majority of their replicative capacity and are not contaminated with non-myogenic cells.
    Type of Medium: Electronic Resource
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