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  • Wheat  (2)
  • Antibody  (1)
  • Springer  (3)
  • Geological Society of America (GSA)
  • Seismological Society of America (SSA)
  • 2000-2004
  • 1995-1999  (3)
Collection
Keywords
Publisher
  • Springer  (3)
  • Geological Society of America (GSA)
  • Seismological Society of America (SSA)
Years
  • 2000-2004
  • 1995-1999  (3)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Low levels of DNA sequence variation among adapted genotypes of hexaploid wheat (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 192-198 
    Details
    ISSN: 1432-2242
    Keywords: Key words PCR markers ; Sequence-tagged-site ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  PCR products from regions corresponding to sequences hybridising to wheat RFLP probes were sequenced in order to establish the level of DNA sequence variation among adapted wheat genotypes. Hexaploid bread wheat shows a very low rate of nucleotide polymorphism, approximately 1 polymorphic nucleotide per 1000 basepairs. Differences in PCR product length can be exploited to design genome-specific amplicons, which may have use in gene tagging or in diagnostic applications. Interpretation of results may be complicated by the simultaneous amplification of orthologous and paralogous sequences. These findings have significant implications for the use of STS markers in wheat and other polyploid species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051224
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Application of two microsatellite sequences in wheat storage proteins as molecular markers (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 247-252 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Microsatellite markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In eukaryotes, tandem arrays of simple-sequence repeat sequences can find applications as highly variable and multi-allelic PCR-based genetic markers. In hexaploid bread wheat, a large-genome inbreeding species with low levels of RFLP, di- and trinucleotide tandem repeats were found in 22 published gene sequences, two of which were converted to PCR-based markers. These were shown to be genome-specific and displayed high levels of variation. These characteristics make them especially suitable for intervarietal breeding applications.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222209
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Cell-based panning as a means to isolate phage display Fabs specific for a bacterial surface protein (1998)
    Springer
    Methods in cell science 20 (1998), S. 241-249 
    Details
    ISSN: 1573-0603
    Keywords: Adhesin ; Antibody ; Bacteria ; Fab ; Phage display
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Surface proteins provide a multitude of functions for the bacterial cell. Antibodies to these proteins can provide tools for tagging bacteria and characterizing protein function. Phage display technology has emerged as a powerful method for producing monoclonal Fabs in Escherichia coli. In an effort to study the adhesion mechanisms of Streptococcus parasanguis FW213, Fabs specific for the surface adhesin protein Fap1 were produced using phage display. The immune repertoire of a mouse injected with purified Fap1 was cloned into the phagemid vector pCOMB3, and a combinatorial Fab library was expressed in E. coli. A cell-based panning method using whole S. parasanguis cells was developed and has been shown to be a means for enriching for Fabs specific for the Fap1 protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009814203184
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    Paper (German National Licenses)
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