ISSN:
1439-0523
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
‘Yi 4060’ is an elite restorer line of a non-photoperiod-sensitive D2-type cytoplasmic male-sterile (CMS) line of wheat. Random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers were employed to map one major fertility-restoring gene (D2Rf1) in ‘Yi 4060′. The sterile and fertile DNA pools were established from individuals in BC6, based on bulked segregant analysis. One RAPD marker E09, linked to D2Rf1, was converted to a SCAR marker and designated as E09-SCAR865. The genetic distance between E09-SCAR865 and D2Rf1 is 9.5 cM. Two SSR markers, Xgwm11 and Xgwm18, were also linked to a D2Rf1 and co-segregated with E09-SCAR865. The three molecular markers are useful in marker-assisted breeding of the elite restorer lines for D2 -type CMS lines in wheat.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1439-0523.2005.01120.x
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