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  • Springer  (102)
  • Wiley-Blackwell  (14)
  • 2005-2009  (42)
  • 1995-1999  (45)
  • 1985-1989  (29)
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  • 1
    ISSN: 1573-4986
    Keywords: influenza virus ; hemagglutinin ; glycoprotein glycans ; oligosaccharide structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Fowl plague virus, strain Dutch, was metabolically labeled withd-[2-3H]mannose, or withd-[6-3H]glucosamine, and the small subunit (HA2; 0.8 mg in total) of the viral hemagglutinin was isolated by preparative sodium dodecylsulfate-polyacrylamide gel electrophoresis. After proteolytic digestion, the radioactive oligosaccharides were sequentially liberated from the glycopeptides by treatment with different endo-β-N-acetylglucosaminidases and with peptide:N-glycosidase or, finally, by hydrazinolysis. In this manner, four groups of glycans could be obtained by consecutive gel filtrations and were subfractionated by HPLC. The structures of the individual oligosaccharides were analyzed by micromethylation, by acetolysis or by digestion with exoglycosidases. The major species amongst the high mannose glycans at Ans-406 of the viral glycopolypeptide were found to be Manα1-2Manα1-3(Manα1-2Manα1-6)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNacβ1-4GlcNAc and Manα1-3(Manα1-2Manα1-6)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc, while the complex glycans at Asn-478 are predominantly GlcNAcβ1-2Manα1-3(GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAc (lacking, in part, one of the outerN-acetylglucosamine residues) and GlcNAcβ1-2Manα1-3(Galβ1-4GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAc.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Glycoconjugate journal 14 (1997), S. 785-793 
    ISSN: 1573-4986
    Keywords: glycoprotein ; glycosylation ; gp120 ; HIV ; MALDI-TOF-MS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Neutral complex-type N-glycans of the envelope glycoprotein 120 of HIV-2, propagated in different host cells, display cell-type specific variations. In order to identify typical structural elements, glycans were analysed by gel filtration, by enzymic sequencing and, in part, by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The characteristic substituents of di- tri- and tetraantennary carbohydrate units thus observed include N-acetyllactosamine repeats, bisecting N-acetylglucosamine and fucose linked to the chitobiose core as well as to N-acetyllactosamine antennae. Each glycoprotein preparation displayed a characteristic set of glycoforms. Abbreviations: endo H, endo-β-N-acetylglucosaminidase H; E-PHA, Phaseolus vulgaris agglutinin E4; GlcNAcOH, N-acetyl-glucosaminitol; gp120/HUT78(MOLT4/Mφ/PBL/U937), external envelope glycoprotein 120 of HIV-2, strain D194, propagated in HUT78 (MOLT4, Mφ, PBL, U937) cells; gu, glucose units; HPAEC, high-pH anion-exchange chromatography; MALDI-TOF-MS, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; Mφ, human monocytes/macrophages; PBL, human peripheral blood lymphocytes; PNGase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase F
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Biogeochemistry 47 (1999), S. 219-238 
    ISSN: 1573-515X
    Keywords: dissolved humic substances ; FTIR spectroscopy ; land use history ; peat ; synchronous fluorescence spectroscopy ; UV/VIS spectroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract The elemental composition and spectroscopic properties of dissolved fulvic acids isolated from different sampling media (topsoil, ground and surface water) of a natural fen area (high portion of organic soils) were examined to reveal the effects of land use history. These effects need to be known if dissolved humic substances are to be a major factor in identifying the impact of present and future changes in land use. Dissolved fulvic acids (topsoil, groundwater) from highly degraded peatlands (due to a long-term agricultural use) exhibit lower C/N ratios, higher absorption in the UV spectra, and higher absorption at 1,620 cm−1 in the FTIR spectra compared with fulvic acids from relatively intact peatlands. These properties illustrate that long-term agricultural use with high inputs results in increased aromatic structures and a further humification of dissolved fulvic acids due to very strong peat decomposition compared with relatively intact peatlands. Synchronous fluorescence spectra also indicate the higher level of aromatic structures within fulvic acids isolated from sites with long-term agricultural use (high peat decomposition) compared with a land use history resulting in a lower peat decomposition. The different sources of fulvic acids in surface water (precipitation, runoff, interflow, groundwater) are the main reason for these effects not being detected in fulvic acids isolated from surface water. Short-term changes in land use characterized by a transition from crop farming to an unimproved grassland were found not to affect the spectroscopic properties of dissolved fulvic acids. A humification index deduced from the synchronous fluorescence spectra is proposed. We have strong evidence that dissolved humic substances indicate changes in the environmental conditions (both anthropogenic and natural) of wetlands with a high proportion of organic soils.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Biogeochemistry 47 (1999), S. 219-238 
    ISSN: 1573-515X
    Keywords: dissolved humic substances ; FTIR spectroscopy ; land use history ; peat ; synchronous fluorescence spectroscopy ; UV/VIS spectroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract The elemental composition and spectroscopic properties of dissolved fulvic acids isolated from different sampling media (topsoil, ground and surface water) of a natural fen area (high portion of organic soils) were examined to reveal the effects of land use history. These effects need to be known if dissolved humic substances are to be a major factor in identifying the impact of present and future changes in land use. Dissolved fulvic acids (topsoil, groundwater) from highly degraded peatlands (due to a long-term agricultural use) exhibit lower C/N ratios, higher absorption in the UV spectra, and higher absorption at 1,620 cm−1 in the FTIR spectra compared with fulvic acids from relatively intact peatlands. These properties illustrate that long-term agricultural use with high inputs results in increased aromatic structures and a further humification of dissolved fulvic acids due to very strong peat decomposition compared with relatively intact peatlands. Synchronous fluorescence spectra also indicate the higher level of aromatic structures within fulvic acids isolated from sites with long-term agricultural use (high peat decomposition) compared with a land use history resulting in a lower peat decomposition. The different sources of fulvic acids in surface water (precipitation, runoff, interflow, groundwater) are the main reason for these effects not being detected in fulvic acids isolated from surface water. Short-term changes in land use characterized by a transition from crop farming to an unimproved grassland were found not to affect the spectroscopic properties of dissolved fulvic acids. A humification index deduced from the synchronous fluorescence spectra is proposed. We have strong evidence that dissolved humic substances indicate changes in the environmental conditions (both anthropogenic and natural) of wetlands with a high proportion of organic soils.
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  • 5
    Publication Date: 1999-11-01
    Print ISSN: 0168-2563
    Electronic ISSN: 1573-515X
    Topics: Chemistry and Pharmacology , Geosciences
    Published by Springer
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  • 6
  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Astrophysics and space science 116 (1985), S. 355-365 
    ISSN: 1572-946X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract U, B, V-observations, supplemented by spectrophotometric measurements of the symbiotic variable CH Cyg in the years 1982 to 1984 show that the star has reached its ‘blue maximum phase’ of its recent outburst in the first half of 1984. From end of July, 1984 on the object underwent a considerable drop in brightness and an increase of its colour indices, and which indicates that it was on the way to its cool phase. Also the spectroscopic observations show that the ‘blue’ continuum still observed at the beginning of July, 1984 has disappeared in October–November, 1984 and the strong absorption features, typical for a M-type giant were re-appearing. A remarkable intensity increase of the emission features, especially those of the Balmer lines, took place within this time interval. The rapid flickering variations of CH Cyg had also reached the culmination amplitude in July, 1984. There seem to be three periodicities present in these flickering variations with cycle lengths of 7.7 min, 21 min, and 60 min. Though these observational findings would be consistent with the M-type blue subdwarf component binary model with mass flow from the M6-III giant to the companion, the genuine binary nature of CH Cyg could not be established beyond doubt.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Astrophysics and space science 131 (1987), S. 595-599 
    ISSN: 1572-946X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The discovery of magnetic field strengths in the range of 500 million Gauss in the objects Grw+70°8247 and PG1031+224 has given enormous impetus to investigations of magnetic white dwarf stars. We have determined intensities of Balmer transitions for B〉108 Gauss as a function of field and find strong oscillations of the transitions strengths, which are of importance to the quantitative analysis of the observed spectra and the interpretation of polarimetric measurements in these objects.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Astrophysics and space science 110 (1985), S. 183-192 
    ISSN: 1572-946X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Beside some historical notes about the large Fraunhofer's heliometer used by Bessel and Argelander, some modern applications of the heliometer principle for the geometric and photometric autocalibration of detectors and the determination of absolute radial velocities with slitless field spectrographs are presented.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Geometriae dedicata 29 (1989), S. 335-375 
    ISSN: 1572-9168
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Type of Medium: Electronic Resource
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