Publication Date:
2000-07-21
Description:
The guanosine triphosphatase Rab1 regulates the transport of newly synthesized proteins from the endoplasmic reticulum to the Golgi apparatus through interaction with effector molecules, but the molecular mechanisms by which this occurs are unknown. Here, the tethering factor p115 was shown to be a Rab1 effector that binds directly to activated Rab1. Rab1 recruited p115 to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacted with a select set of COPII vesicle-associated SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. We propose that Rab1-regulated assembly of functional effector-SNARE complexes defines a conserved molecular mechanism to coordinate recognition between subcellular compartments.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Allan, B B -- Moyer, B D -- Balch, W E -- CA58689/CA/NCI NIH HHS/ -- GM 33301/GM/NIGMS NIH HHS/ -- GM42336/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2000 Jul 21;289(5478):444-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Departments of Cell and Molecular Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10903204" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Biological Transport
;
Carrier Proteins/*metabolism
;
Endoplasmic Reticulum/*metabolism
;
Golgi Apparatus/*metabolism
;
Intracellular Membranes/metabolism
;
Membrane Fusion
;
*Membrane Glycoproteins
;
Membrane Proteins/*metabolism
;
Mutation
;
Organelles/metabolism
;
Phosphoproteins/*metabolism
;
Rats
;
Recombinant Fusion Proteins/metabolism
;
SNARE Proteins
;
*Saccharomyces cerevisiae Proteins
;
*Vesicular Transport Proteins
;
Viral Envelope Proteins/metabolism
;
rab1 GTP-Binding Proteins/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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