Publication Date:
2010-02-27
Description:
The nitrogen-fixing symbiosis between Sinorhizobium meliloti and its leguminous host plant Medicago truncatula occurs in a specialized root organ called the nodule. Bacteria that are released into plant cells are surrounded by a unique plant membrane compartment termed a symbiosome. We found that in the symbiosis-defective dnf1 mutant of M. truncatula, bacteroid and symbiosome development are blocked. We identified the DNF1 gene as encoding a subunit of a signal peptidase complex that is highly expressed in nodules. By analyzing data from whole-genome expression analysis, we propose that correct symbiosome development in M. truncatula requires the orderly secretion of protein constituents through coordinated up-regulation of a nodule-specific pathway exemplified by DNF1.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wang, Dong -- Griffitts, Joel -- Starker, Colby -- Fedorova, Elena -- Limpens, Erik -- Ivanov, Sergey -- Bisseling, Ton -- Long, Sharon -- Howard Hughes Medical Institute/ -- New York, N.Y. -- Science. 2010 Feb 26;327(5969):1126-9. doi: 10.1126/science.1184096.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biology, Stanford University, Stanford, CA 94305, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20185723" target="_blank"〉PubMed〈/a〉
Keywords:
Endoplasmic Reticulum/metabolism
;
Gene Expression
;
Gene Expression Profiling
;
Genes, Plant
;
Medicago truncatula/genetics/*metabolism/*microbiology
;
Membrane Proteins/genetics/*metabolism
;
Mutation
;
*Nitrogen Fixation
;
Plant Proteins/genetics/metabolism
;
Plants, Genetically Modified
;
Protein Subunits/genetics/metabolism
;
Root Nodules, Plant/metabolism/*microbiology
;
*Secretory Pathway
;
Serine Endopeptidases/genetics/*metabolism
;
Sinorhizobium meliloti/*physiology
;
*Symbiosis
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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