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  • 1
    Publication Date: 2022-05-25
    Description: © The Author(s), 2013. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Frontiers in Neural Circuits 7 (2013): 160, doi:10.3389/fncir.2013.00160.
    Description: Here we characterize several new lines of transgenic mice useful for optogenetic analysis of brain circuit function. These mice express optogenetic probes, such as enhanced halorhodopsin or several different versions of channelrhodopsins, behind various neuron-specific promoters. These mice permit photoinhibition or photostimulation both in vitro and in vivo. Our results also reveal the important influence of fluorescent tags on optogenetic probe expression and function in transgenic mice.
    Description: This work was supported by a CRP grant from the National Research Foundation of Singapore and by the World Class Institute (WCI )Program of the National Research Foundation of Korea (NRF )funded by the Ministry of Education, Science and Technology of Korea (MEST) (NRF Grant Number: WCI2009-003).
    Keywords: Optogenetics ; Channelrhodopsin ; Photostimulation ; Photoinhibition ; Cerebellum ; Cortex ; Hippocampus ; Pons
    Repository Name: Woods Hole Open Access Server
    Type: Article
    Format: application/pdf
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  • 2
    Publication Date: 2022-05-26
    Description: Author Posting. © American Geophysical Union, 2010. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 115 (2010): C08003, doi:10.1029/2009JC005702.
    Description: The estuarine boundary layer affected by a horizontal density gradient exhibits temporal evolution over a tidal cycle, in a manner similar to the diurnal cycle of the ocean surface mixed layer. A large eddy simulation (LES) model is developed to investigate the physics controlling the growth of the boundary layer during the flood tide and restratification during the ebb tide. Turbulent kinetic energy, momentum and salt fluxes, bottom stress, and energy dissipation rates calculated from the LES model all show a strong flood-ebb asymmetry. Analysis of the turbulent kinetic energy (TKE) budget shows a primary balance between shear production and dissipation in the well-mixed boundary layer over the tidal cycle. However, TKE transport term is found to be important across the edge of the boundary layer during the flood tide so turbulent energy generated in the bottom boundary layer can be transferred to the stratified pycnocline region. Tidal straining leads to a small and weakly convective region inside the boundary layer during the flood tide but the strain-induced buoyancy flux does not make a significant contribution to the turbulence generation. Additional LES runs are conducted by switching off the baroclinic pressure gradient term in the momentum equation and the tidal straining term in the salinity equation to show that the baroclinic pressure gradient is the main mechanism responsible for generating the flood-ebb mixing asymmetry.
    Description: This work is supported by grants OCE-0451699 (M.L.), OCE-0452380 (U.P. and S.R.), and OCE-0451740 (W.R.G.) from the National Science Foundation.
    Keywords: Estuarine mixing ; Large Eddy Simulations ; Tidal straining
    Repository Name: Woods Hole Open Access Server
    Type: Article
    Format: application/pdf
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